FastQCFastQC Report
Fri 17 Jun 2016
SRR1526607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151776
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4940.3254796542272823No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.32350305713683325No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3380.22269660552393No Hit
AGATGGGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGT3330.2194022770398482No Hit
AGATGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.1739405439595193No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2620.17262281256588657No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.15285684166139574No Hit
AGATGGGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT2310.1521979759645794No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.151539110267763No Hit
AGATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG2120.13967952772506853No Hit
AGATGGGGGAGGCCTTTAGAGCGATGGAATGGCCATTGTCATAGCACAGAGATTTCAGAAGCAC2050.135067467847354No Hit
AGATGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1700.11200716845878135No Hit
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.10937170567151591No Hit
AGATGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1600.10541851149061775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA258.521534E-658.12773551
ACCGTAT258.521534E-658.12773542
CCGCAAG201.9830829E-458.1277347
GAACTAT150.00463879858.12772852
TCCCGAT150.00463879858.12772847
GTAAGCA150.00463879858.12772855
CCTACCG258.538274E-658.108539
GTAAGGA150.004644880558.108540
TACCGTA258.538274E-658.108541
GTAGACA201.9863306E-458.10849840
GTTCTAA150.00465706458.0700837
CGACAGG150.00465706458.0700835
TACGACC150.004669271458.0317134
TGACGAC150.004675383758.01254732
CGGGTAG202.0059064E-457.99339730
AACGACA150.004681502557.99339330
GATAACC150.00470603657.9169124
CGAGATA150.00471218457.89782318
TTCGGCA258.72421E-657.89782319
TTTAGTA150.004718338657.87874612
Produced by FastQC (version 0.11.5)