FastQCFastQC Report
Fri 17 Jun 2016
SRR1526606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146251
Sequences flagged as poor quality0
Sequence length64
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.2748699154193817No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3920.2680323553343225No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2570.17572529418602267No Hit
AGATGGGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGT2520.17230651414349304No Hit
AGATGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.15316134590532712No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2230.15247758989682123No Hit
AGATGGGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT2050.14016998174371456No Hit
AGATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG2040.13948622573520864No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.12922988560761978No Hit
AGATGGGGGAGGCCTTTAGAGCGATGGAATGGCCATTGTCATAGCACAGAGATTTCAGAAGCAC1750.11965730148853684No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.11692227745451313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA150.004589948358.2821734
CGGGTGC150.004627691658.16179338
CCTACCG150.004627691658.16179339
AGGAGCG258.490355E-658.1617939
AGCGAGT303.684927E-758.12177730
TGGTAAG150.004640323658.12177731
CGGGTAG258.525087E-658.12177330
CTAGCGG258.525087E-658.12177330
CACGCAC150.00464664958.10178853
CTAAGCA201.9872119E-458.10178858
TACGTGC150.00464664958.10178853
CGTGCCG150.0046593258.0618628
CTAGTTG150.0046593258.0618648
CTCATAA201.993981E-458.06185548
GTAGATT150.004665665358.0419145
CTACCGT150.004672016558.0219840
CCCTTAT150.004672016558.0219842
TACCGTA150.004672016558.0219841
AACGGGA150.004678374658.0020627
TAACGGG150.00468473957.9821626
Produced by FastQC (version 0.11.5)