FastQCFastQC Report
Fri 17 Jun 2016
SRR1526605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130646
Sequences flagged as poor quality0
Sequence length56
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6640.5082436507815011No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4730.3620470584633284No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3180.243405844801984No Hit
AGATGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.2227393108093627No Hit
AGATGGGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAG2610.19977649526200575No Hit
AGATGGGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCA2020.15461629135220367No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.15385086416729177No Hit
AGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.14466573794834897No Hit
AGATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1890.14466573794834897No Hit
AGATGGGGGAGGCCTTTAGAGCGATGGAATGGCCATTGTCATAGCACAGAGATTTC1860.14236945639361326No Hit
AGATGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1710.13088804861993478No Hit
AGATGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1710.13088804861993478No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.1186412136613444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT203.8895285E-450.7102737
ACGTGTA150.00795774750.7102737
CATAGAA150.00798235550.670736
AAGCGGT150.0079946850.65093635
TGATAAC150.00801937350.61145834
ATTACGT150.00801937350.61145834
TATCGAG203.9271728E-450.61145834
TATTACG150.0080937950.4933933
ACTATAA203.9956617E-450.43456631
CGGTAGG251.9734207E-550.43456631
GCGAGTT354.8950824E-850.43456331
GGTTCTA150.00824416550.258940
TCGGCTG204.0806556E-450.2200338
AAATCTG150.0082947550.1812250
CTACGTG150.0082947550.1812250
GCTACGT150.0082947550.1812249
GTATTCA150.00830743250.1618443
AAGGTAT150.00830743250.1618446
CGCTGTT150.00830743250.1618443
TCGTGTA150.00830743250.1618443