FastQCFastQC Report
Fri 17 Jun 2016
SRR1526604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193104
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT17470.904693843731875No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.880354627558207No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.4148023862788963No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.30242770734940755No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4720.24442787306321984No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.16105311127682492No Hit
GTGGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.1589816886237468No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.1517317093379733No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2920.15121385367470377No Hit
GTGGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.14862457535835613No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.13982102908277405No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2580.13360676112353964No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2490.12894606015411383No Hit
GTGGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.12894606015411383No Hit
GTGGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.12221393653160992No Hit
GTGGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.11392824591929737No Hit
GTGGTAGGGGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTCTTTTTTTCTT2090.10823183362333251No Hit
GTGGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.1035711326539067No Hit
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1950.10098185433755905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATA150.00463529158.144949
TGCGAAA407.003109E-1058.12978444
CTAAACG150.00464006958.1297846
TTAGCGT150.00468323157.99403434
AGTCGTG150.00468323157.99403434
AAGTCGT150.00468323157.99403433
GTCTATG150.00468804557.9789932
AACGTGG258.6876225E-657.94892529
CGACGTA150.004697684757.94892527
CTTTTAC150.004697684757.94892529
TTAGCCG202.0149145E-457.9489230
GACGTAT202.0149145E-457.9489228
GTACTAT202.0149145E-457.9489227
ACCGCAA150.0047025157.93390725
AACGACG150.0047025157.93390725
TTCGGTA150.0047025157.93390725
ACGTACG150.0047025157.93390726
AAGTCTA150.004707338857.9188921
CTCTTTA150.004712171457.9038920
GGATATC150.00472184857.87398
Produced by FastQC (version 0.11.5)