FastQCFastQC Report
Fri 17 Jun 2016
SRR1526603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188997
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13900.7354614094403615No Hit
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13580.7185299237554036No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.35767763509473693No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.25344317634671454No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3870.20476515500246037No Hit
GTGGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.12381148907125511No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.11799129086705079No Hit
GTGGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.11640396408408599No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.1148166373011212No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2110.11164198373519157No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.11164198373519157No Hit
GTGGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.11111287480753662No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1930.10211802303740272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAC201.950199E-458.33199734
TTCGCTA201.9527784E-458.3164133
CATGGGA150.004586218458.30082737
ACCATAC150.004586218458.30082737
ATAGCCG150.004586218458.30082737
CTACCGT150.004586218458.30082737
ACGGCGT201.9605333E-458.26969538
CGGGATA150.004595931658.2696935
CATACAC150.004595931658.2696939
TACCGTA150.004595931658.2696938
CTAGTAG150.004620281558.19199858
GCTGCAT201.9761165E-458.1764956
GATTGGA201.9787233E-458.1609855
CACTCTA258.514122E-658.1454953
CGACACT258.5545635E-658.09904550
AGTTTTC351.6096237E-858.0990448
ACGCACC201.9891777E-458.0990450
CACTCGC150.00464962858.0990450
TCCACGG150.00464962858.0990431
ATGAAAT150.00464962858.0990431
Produced by FastQC (version 0.11.5)