FastQCFastQC Report
Fri 17 Jun 2016
SRR1526602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174518
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18261.0463104092414535No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG17731.0159410490608418No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.5391993949048236No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.37531945128869226No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG4400.2521229901786635No Hit
GTGGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.16387994361613129No Hit
GTGGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.1610149096368283No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2700.1547118348823617No Hit
GTGGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.14554372614859212No Hit
GTGGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG2320.13293757663965894No Hit
GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.13007254266035595No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2170.12434247470174996No Hit
GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.12434247470174996No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1950.11173632519281679No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1870.107152270825932No Hit
GTGGTAGGGGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTCTT1830.10486024364248961No Hit
GTGGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.10084919607146542No Hit
GTGGTAGGGGTCAGATGAATACATCCACTGGGATTCATTAGAGATGCTCAGTGAAT1750.10027618927560482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACATA203.9080603E-450.6712437
CGAAGAA251.9222036E-550.6712437
TTCATTA309.548476E-750.6416734
GCGAAGA251.928858E-550.64166636
AGGATTT251.928858E-550.64166635
AATTGTG150.00807918550.52372733
ATGCGTT309.703399E-750.52372733
CTATTTA251.9556668E-550.52372433
TATGCGT309.742471E-750.49432832
TGCGATC150.00809780350.49432832
ACCGTCT150.00809780350.49432832
GTATGCG402.4447218E-950.4796431
CCTAATC150.00827626350.2167340
CAAGCTA150.00827626350.2167340
CATAACT150.00827626350.2167340
GAAATAG150.00828573650.202238
TTAGAGA301.0140539E-650.202238
TCCCTAA150.00828573650.202238
ACATAAC150.00828573650.202239
CGCTAGT252.0309875E-550.20219839
Produced by FastQC (version 0.11.5)