##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526602_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174518 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.982718115036846 38.0 37.0 38.0 33.0 38.0 2 35.29987737654569 38.0 34.0 38.0 30.0 38.0 3 36.69816867028043 38.0 37.0 38.0 33.0 38.0 4 36.574244490539655 38.0 37.0 38.0 33.0 38.0 5 32.368397529194695 34.0 31.0 36.0 24.0 38.0 6 37.02874775094833 38.0 38.0 38.0 34.0 39.0 7 37.14048407614114 38.0 38.0 38.0 35.0 39.0 8 37.398577797132674 38.0 38.0 38.0 35.0 39.0 9 37.28660653915355 38.0 38.0 38.0 35.0 39.0 10 35.67383880172819 38.0 35.0 38.0 30.0 39.0 11 36.35239917945427 38.0 35.0 39.0 32.0 39.0 12 36.156556916765034 38.0 35.0 39.0 31.0 39.0 13 36.11085962479515 38.0 35.0 39.0 31.0 39.0 14 36.15758832899758 38.0 35.0 39.0 31.0 39.0 15 36.22657261715124 38.0 35.0 39.0 31.0 39.0 16 35.883582209285 38.0 35.0 39.0 31.0 39.0 17 35.83045301917281 38.0 35.0 39.0 31.0 39.0 18 35.68367732841312 38.0 35.0 39.0 30.0 39.0 19 35.47754959373818 38.0 35.0 39.0 30.0 39.0 20 35.51532220172131 38.0 35.0 39.0 30.0 39.0 21 35.00477887667748 38.0 34.0 39.0 29.0 39.0 22 34.93860805189149 37.0 34.0 39.0 29.0 39.0 23 34.62755131275857 37.0 33.0 38.0 28.0 39.0 24 34.456480133854384 37.0 33.0 38.0 27.0 39.0 25 34.300811377622935 36.0 33.0 38.0 27.0 39.0 26 33.602539566119255 36.0 33.0 38.0 25.0 39.0 27 33.57533320345179 36.0 33.0 38.0 25.0 39.0 28 33.26211049863051 36.0 32.0 38.0 23.0 39.0 29 33.155158780183136 36.0 32.0 38.0 23.0 39.0 30 32.87492980666751 36.0 32.0 38.0 23.0 39.0 31 32.04774865629906 36.0 30.0 38.0 17.0 39.0 32 31.789901328229753 35.0 30.0 38.0 16.0 39.0 33 31.799969057633025 35.0 30.0 38.0 17.0 39.0 34 31.891988218980277 35.0 30.0 38.0 17.0 39.0 35 31.544608579057748 35.0 30.0 38.0 15.0 39.0 36 31.654488362231977 36.0 30.0 39.0 4.0 39.0 37 31.201469189424586 35.0 30.0 39.0 4.0 39.0 38 31.26434522513437 35.0 30.0 39.0 4.0 39.0 39 31.130255904835032 35.0 30.0 39.0 4.0 39.0 40 30.6933554131952 35.0 29.0 39.0 4.0 39.0 41 30.16162229684044 35.0 29.0 39.0 4.0 39.0 42 30.070840830172244 35.0 28.0 39.0 4.0 39.0 43 29.795597015780608 35.0 28.0 39.0 4.0 39.0 44 29.351482368580893 35.0 27.0 39.0 4.0 39.0 45 29.139487044316347 35.0 26.0 38.0 4.0 39.0 46 28.472489943730732 35.0 25.0 38.0 4.0 39.0 47 28.183746089228617 34.0 24.0 38.0 4.0 39.0 48 27.846502939524864 34.0 23.0 38.0 4.0 39.0 49 27.662332825267306 34.0 22.0 38.0 4.0 39.0 50 27.23815881456354 34.0 19.0 38.0 4.0 39.0 51 26.5168463997983 33.0 4.0 38.0 4.0 39.0 52 26.28633149589154 33.0 4.0 38.0 4.0 39.0 53 25.885501782051136 33.0 4.0 38.0 4.0 39.0 54 25.516084300759807 33.0 4.0 38.0 4.0 39.0 55 24.866317514525722 33.0 4.0 37.0 4.0 39.0 56 14.552097777879645 17.0 4.0 22.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.38482944548595555 1 2 -0.7571227481995777 1 3 -0.7469986547858909 1 4 -0.8549255574435719 1 5 1.0785021765454914 1 6 -0.4287956740788985 1 7 -0.34084015406308765 1 8 -0.8798081489147052 1 9 -0.7321289174415426 1 10 0.22516947037500046 1 11 -0.5791956788600245 1 12 -0.3877663703927041 1 13 -0.7073652012628671 1 14 -0.7009971268946273 1 15 -0.38815447694940275 1 16 -0.3932741416949881 1 17 -0.6227714626779317 1 18 -0.5215504633371353 1 19 -0.3540031255184246 1 20 -1.2622000778762867 1 21 -0.6695258593561491 1 22 -1.1043990216143769 1 23 -0.5401247780642819 1 24 -0.13632276227229312 1 25 -0.8288136539590951 1 26 0.049054476956968074 1 27 -0.29598532496649455 1 28 0.0982636758816895 1 29 0.2454581671480227 1 30 -0.19139348322877936 1 31 0.6016991749304559 1 32 0.438636180354365 1 33 0.6054366372263082 1 34 0.7375174625572356 1 35 0.7854062551199696 1 36 0.37866463320819577 1 37 0.8851182619508258 1 38 0.7064906502745743 1 39 0.7835464854440488 1 40 0.9103937259407111 1 41 1.0488691916396853 1 42 0.6012508891007826 1 43 0.8576382101019284 1 44 1.5515130900765328 1 45 1.1150868548601416 1 46 0.7708275354145435 1 47 1.2511698077622277 1 48 1.202113484314637 1 49 1.6280658414296347 1 50 1.961039986763634 1 51 1.643589491472266 1 52 1.7505556742775177 1 53 2.016990869532222 1 54 2.098800992847149 1 55 1.989540372353332 1 56 1.1686040701407805 2 1 -0.1036248010737637 2 2 0.3944253891976217 2 3 -0.2704626122121141 2 4 0.03501154947466745 2 5 0.601361392800932 2 6 0.14171714643391908 2 7 0.47701180529830367 2 8 0.3267714350233675 2 9 0.3102029645129818 2 10 0.5015361854209601 2 11 0.2814719553925187 2 12 0.12711026240845769 2 13 0.3042458292160859 2 14 -0.09867492079883533 2 15 -0.032203823828936606 2 16 0.0010171016528559562 2 17 0.27345495241640805 2 18 -0.15906860557225855 2 19 -0.08549804569258868 2 20 -0.3351318630722204 2 21 0.09123853348371824 2 22 0.12588641621822916 2 23 -0.26460470065740793 2 24 -0.1984901546283666 2 25 0.2885061331720138 2 26 -0.22199051578613904 2 27 -0.11347443962542059 2 28 0.6249932356301215 2 29 0.39470828809626113 2 30 0.8088484132395308 2 31 1.1951921599328763 2 32 0.895457660760755 2 33 1.4235304930560169 2 34 1.1052000943908098 2 35 1.391598804708746 2 36 1.4116835011327247 2 37 1.6462454994931583 2 38 1.4972502051850753 2 39 1.6247414539975082 2 40 2.442807852694461 2 41 1.7278725781902402 2 42 1.9195866413988014 2 43 1.3898104403873681 2 44 1.7767187020746 2 45 1.8994361533603836 2 46 1.865650950992677 2 47 1.9582331846369243 2 48 1.9615474465787877 2 49 1.4142293634422138 2 50 1.6059359712822605 2 51 1.5522735746846479 2 52 1.3407830569577293 2 53 1.6986551172342033 2 54 1.4651531941050102 2 55 1.3183986210229008 2 56 0.6485266632709212 3 1 -0.11841019222460147 3 2 0.31586420636632795 3 3 -0.04614395393119253 3 4 0.06612707413537322 3 5 -0.2508950344306662 3 6 0.3639393686561405 3 7 0.5199335751316951 3 8 0.23265248986172082 3 9 0.08456024135288231 3 10 0.6094474730740558 3 11 -0.648021589791199 3 12 -0.6431644810539723 3 13 -0.35513848061509634 3 14 -0.017686285689045178 3 15 -0.2771558264770704 3 16 -0.7940838582941794 3 17 -0.2046428077071667 3 18 -1.076093873143698 3 19 -0.38054383626965915 3 20 -1.3322630558340052 3 21 -0.5419954949836807 3 22 -0.852487190755177 3 23 -0.6217712018159673 3 24 -1.0803173641616297 3 25 -0.5640813102793842 3 26 -0.585335986380862 3 27 -0.6638440744041958 3 28 -1.0727889556972556 3 29 -0.38814507171836965 3 30 -0.8510875902913071 3 31 -0.6215351930407564 3 32 -1.1626908551831932 3 33 -1.4157059628636581 3 34 -1.6232562666375436 3 35 -1.6360962190140818 3 36 -0.8596394603590554 3 37 -0.8394656336857018 3 38 -0.6230190213403581 3 39 -0.8728862630939638 3 40 -1.3336458602041397 3 41 -0.3733080463270291 3 42 -0.14098932682364307 3 43 -0.38167802921431004 3 44 -0.33715087753480333 3 45 -0.5424705000656367 3 46 -0.5953443044415074 3 47 -0.3212548436097933 3 48 -0.9630911940479372 3 49 -0.8163786030148081 3 50 -1.1635664010006508 3 51 -1.049955267861968 3 52 -1.1486795933788052 3 53 -1.0653303180521263 3 54 -1.5313159662171785 3 55 -0.809042623598085 3 56 -1.3019312424997942 4 1 -0.24805156473217238 4 2 0.21468756999628624 4 3 -0.12914261524310433 4 4 0.09085757508654524 4 5 -0.5995175948477716 4 6 0.021235218723077764 4 7 0.21013296919919355 4 8 0.12111863514460453 4 9 0.03154114743334446 4 10 0.3766214320679211 4 11 -0.6284696980135607 4 12 -0.508943386148033 4 13 -0.357040826842109 4 14 -0.9546270311269396 4 15 -0.2496774920868745 4 16 -0.787002902893633 4 17 -1.060522466693996 4 18 -0.6794899134452592 4 19 -0.6252419268777061 4 20 -0.8223638097267667 4 21 -0.1602580187630096 4 22 -0.29083708154641386 4 23 -0.8572393903596165 4 24 -0.5336041956983024 4 25 -0.4642785906288509 4 26 -0.7219526283874842 4 27 -0.41808912478114024 4 28 -0.7882825088572645 4 29 -1.093281406531304 4 30 -0.35126373346047757 4 31 -0.6600400898208569 4 32 0.44334733265588255 4 33 0.3399130042327947 4 34 -0.28132714664881675 4 35 -0.37755327530943816 4 36 0.4198292918427313 4 37 0.7168297231803571 4 38 0.49224906856311534 4 39 1.4913778107452558 4 40 0.8050492711986657 4 41 1.186651088457726 4 42 1.0975437528635261 4 43 0.9503166160333478 4 44 1.0436354255723614 4 45 0.6574872255737638 4 46 1.621769765874845 4 47 1.2877777781051378 4 48 1.7051255325074095 4 49 1.8328851185380692 4 50 0.5320934313110541 4 51 0.5628820463069886 4 52 1.2856190045215712 4 53 1.5086498129983923 4 54 1.5536202699555837 4 55 1.2262564675031626 4 56 0.9117304217008666 5 1 -0.30499985478680003 5 2 0.03269250900698495 5 3 0.0249395519349207 5 4 0.0736458711278587 5 5 -0.230663295663458 5 6 -0.0632517976654583 5 7 0.614009344264673 5 8 0.16414789504133864 5 9 0.04355192345668968 5 10 -0.2433366611746095 5 11 0.33639597586206804 5 12 0.39758957800672334 5 13 0.11298835822112352 5 14 -0.14230943777215543 5 15 0.3074578817064335 5 16 0.3695861863827261 5 17 0.0925494482951521 5 18 0.4746670872282479 5 19 0.12728370359449315 5 20 0.34032420943061226 5 21 0.07127681622913684 5 22 0.7963813574268741 5 23 0.3026049559698052 5 24 0.5191160018621233 5 25 0.5858224345900354 5 26 0.12052286364274778 5 27 0.15348911363837203 5 28 -0.06995578581602757 5 29 0.06461726891582487 5 30 -0.4353017488761779 5 31 0.7524795539717068 5 32 0.4037978302986289 5 33 0.6517815966147502 5 34 0.6843242789292745 5 35 0.4001538263892819 5 36 0.8595134944918392 5 37 -1.4749311089681107 5 38 0.24200585970282873 5 39 0.3130495910341118 5 40 0.5362099387134442 5 41 0.2720735235396674 5 42 0.3881596325312664 5 43 0.7563481957684388 5 44 0.8517997599284222 5 45 0.8884982260975995 5 46 1.454853654223271 5 47 1.5495453452682 5 48 1.4334799439419648 5 49 1.1435937917401944 5 50 0.7119557376793857 5 51 2.0460819265733967 5 52 1.746892670614514 5 53 1.2549268465986323 5 54 1.6127761481266525 5 55 2.195425067368461 5 56 0.7411474687711355 6 1 0.11650098508975759 6 2 0.645997716241645 6 3 0.11318518653002485 6 4 0.06645809035516947 6 5 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46 2.8719402591687775 6 47 2.8953401028759167 6 48 3.30117008808822 6 49 3.565172759668627 6 50 2.6373825121627625 6 51 3.3447264048733274 6 52 3.4791478368319417 6 53 3.0697242996241414 6 54 3.043769546306269 6 55 2.475631325425418 6 56 -0.15021547509385869 7 1 0.31531300465648826 7 2 0.947768040024549 7 3 0.3427449349577003 7 4 0.35495098576630113 7 5 0.3888060701827172 7 6 0.413322084705527 7 7 0.6781531982636011 7 8 0.3837246146180533 7 9 0.54849786318524 7 10 0.5824819110207713 7 11 0.31396671430237433 7 12 0.4253865260935257 7 13 0.3648095375785374 7 14 0.5554131295156282 7 15 0.48957581744755885 7 16 0.825149410061897 7 17 0.8587100188035492 7 18 1.0233213315346603 7 19 0.45084017932487797 7 20 0.05142765241811276 7 21 0.1324684426381495 7 22 -0.23260414981950817 7 23 0.08713068252451706 7 24 -0.06699702627989268 7 25 -0.2713587631708734 7 26 -0.12497211373629113 7 27 0.2906718450240078 7 28 0.6630784906965062 7 29 0.7976898860464132 7 30 0.012785054283092734 7 31 0.4827533059845841 7 32 1.0699277283125852 7 33 1.31009000854635 7 34 1.2493883077614143 7 35 0.5617595314732462 7 36 2.1471831517267113 7 37 2.0952322220647837 7 38 2.159956937074192 7 39 2.6323119175428147 7 40 2.4555029377165916 7 41 2.6533326294364556 7 42 3.331070452253684 7 43 3.1244948102918606 7 44 3.0182747235048346 7 45 3.2164638729038266 7 46 3.7717772030308794 7 47 3.4230596463187304 7 48 3.50149620036402 7 49 3.566337446367907 7 50 3.1528728452631576 7 51 2.840882938765713 7 52 2.088162511884356 7 53 2.41062809650278 7 54 2.7747269187730765 7 55 3.0733485394948303 7 56 1.2217854566554998 8 1 0.05524859241816671 8 2 0.10101549037344881 8 3 -0.24835237353091344 8 4 -0.14995661334419452 8 5 -0.15612913417277596 8 6 -0.1502083194045909 8 7 0.34692859892305705 8 8 0.039924292556861474 8 9 0.124297265071597 8 10 0.023744084167006463 8 11 -0.2785108564360712 8 12 -0.5899880617448261 8 13 0.13783307871801753 8 14 -0.4342349036106654 8 15 -0.2018588884799044 8 16 0.07766072054422324 8 17 -0.15589775667378802 8 18 -0.03464968295809001 8 19 0.15212842409138716 8 20 -0.10688075951348708 8 21 -0.7796548607894991 8 22 -0.7564136736290266 8 23 -0.504848460179268 8 24 -0.13305791118135346 8 25 0.4615988321055653 8 26 -0.18382496244421276 8 27 0.04168787140235253 8 28 0.3710380030038465 8 29 -0.09687341431398977 8 30 -0.36076758738548875 8 31 -0.16677510441338939 8 32 0.3230524886837216 8 33 -0.44138610524860766 8 34 -0.5517005657912257 8 35 0.6423898512340038 8 36 0.6980228112620246 8 37 1.3635782078020746 8 38 0.5836207665351267 8 39 0.7788881003508799 8 40 1.1697503113842522 8 41 1.5950548899992967 8 42 1.2887807505436903 8 43 1.4457891895572565 8 44 1.4667065922265579 8 45 1.3481255552901459 8 46 1.7157232194406618 8 47 1.5681788856408687 8 48 2.0049085153705377 8 49 2.2678173942246644 8 50 1.7119557376793857 8 51 1.7231005601137674 8 52 1.7531038507387358 8 53 1.391572809331553 8 54 1.5382419866359704 8 55 0.1395244462504479 8 56 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-0.4131408716428311 120 31 -0.054283731052450435 120 32 0.4649894567076416 120 33 0.2792258110154826 120 34 0.5950673201070984 120 35 0.4568212598683097 120 36 0.7706399418501668 120 37 0.7186890121882428 120 38 1.1476112580618505 120 39 0.7372501891477512 120 40 1.140688122901775 120 41 1.6039499133870727 120 42 1.1335395880561485 120 43 1.686223205353592 120 44 1.1479043531344644 120 45 1.2041181938914818 120 46 1.7841228820432242 120 47 2.083553473479224 120 48 2.2113727435738966 120 49 1.5786831253802518 120 50 1.6960827218063699 120 51 1.2853273832101557 120 52 1.0881625118843559 120 53 1.5032206890953717 120 54 1.2623812397607281 120 55 1.752360885173843 120 56 0.8143780492480932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 370.0 5 23.0 6 40.0 7 110.0 8 274.0 9 270.0 10 258.0 11 410.0 12 445.0 13 583.0 14 805.0 15 1003.0 16 1180.0 17 1516.0 18 1868.0 19 1837.0 20 2234.0 21 2321.0 22 2057.0 23 2571.0 24 3207.0 25 3132.0 26 3597.0 27 4017.0 28 4664.0 29 5577.0 30 6715.0 31 7988.0 32 9688.0 33 12107.0 34 15116.0 35 18057.0 36 22119.0 37 24910.0 38 11319.0 39 2130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.3332721831753 0.37779332010961164 0.21842072073105012 0.07051377598403981 8 99.29348262070388 0.5202901706414238 0.09168108733769582 0.09454612131699881 9 97.83116927766763 1.4147537789798186 0.3294789076198444 0.42459803573270377 10 46.50351253165862 34.75973825049565 7.649067717943135 11.087681499902589 11 29.60955316930059 32.55423509322821 20.60074032478025 17.235471412690956 12 39.64003713084037 16.96157416426959 21.80577361647509 21.59261508841495 13 24.624394045313377 16.902554464295946 29.741917739144384 28.73113375124629 14 26.708419761858377 23.7660298651142 27.874488591434694 21.65106178159273 15 32.12562600992448 20.192186479331646 25.347528621689452 22.334658889054424 16 28.40451987760575 22.473326533652692 26.51073241728647 22.611421171455092 17 32.16115243126784 20.92277014405391 22.350703079338523 24.565374345339734 18 28.24006692719376 22.048728497919985 25.656379284658314 24.054825290227942 19 28.28361544367916 22.568445661765548 25.070766339288785 24.077172555266504 20 26.42248937072394 25.954342818505825 21.8332779426764 25.789889868093834 21 26.76113638707755 20.931365245991817 29.49781684410777 22.80968152282286 22 30.310340480638096 20.251206179305285 23.905270516508324 25.53318282354829 23 29.511334204313645 24.295193563913912 24.842421325265303 21.35105090650714 24 28.31414704584654 25.596360239976622 24.917916306147823 21.171576408029015 25 29.319704882509072 21.944633940804522 25.091292658178503 23.644368518507903 26 27.503741206015746 21.686954228279504 26.43326892534216 24.376035640362595 27 25.272897271314093 21.54497427337337 26.267545444637562 26.914583010674974 28 26.784372436658575 24.41190136118807 27.007508618563325 21.79621758359003 29 28.661272737705858 21.654329488724393 27.842428415676824 21.841969357892925 30 24.584647014569658 24.130386093068932 30.857582596810413 20.427384295551 31 24.787802216734622 24.452420605294893 30.98374777465112 19.77602940331936 32 24.058067630629182 22.571320199535645 35.08425093676008 18.2863612330751 33 24.30081688732014 22.290503756761883 33.44006714457353 19.968612211344443 34 21.726382117560483 25.56112593137706 35.75855487075706 16.9539370803054 35 19.79113352617444 27.845029357471056 36.6419193632793 15.721917753075202 36 21.060110924447102 26.778933560398592 34.32386716682392 17.83708834833038 37 20.254943523331107 25.4972560178867 36.33613054966753 17.911669909114668 38 22.571191690752276 25.16092258443398 35.751874071600476 16.51601165321327 39 21.316845420056072 24.308158178039527 35.10589914969805 19.26909725220635 40 21.22360678435445 24.471558786200532 36.530518056997806 17.774316372447213 41 19.18118346467579 24.618069957140882 38.10198626664823 18.098760311535095 42 20.53143854917622 23.59432717297973 36.53049346218788 19.343740815656176 43 20.75999285092448 23.456462706616932 38.08842945188499 17.6951149905736 44 20.319412161655546 23.338197380335792 36.98040731106638 19.361983146942283 45 19.659366835272202 24.730221532676214 37.01573972073386 18.594671911317718 46 19.453804786853464 24.605326932397812 36.07854224848247 19.862326032266253 47 19.09662532448803 26.449956734929337 36.82319007787713 17.630227862705507 48 20.612023246946816 26.157436827692983 35.63068319680181 17.59985672855839 49 19.922272013304998 27.07486371616003 35.48692599094521 17.51593827958976 50 19.81625629417471 25.969533884602946 35.58518501408971 18.629024807132627 51 18.010034120628834 28.18098366713046 36.5292796563689 17.279702555871808 52 19.69990123770525 26.156991619643875 36.17241239899967 17.9706947436512 53 19.289560033274793 26.109737498844627 36.45900730196876 18.141695165911823 54 20.335937319435136 26.76289088681905 34.35932696974599 18.541844823999813 55 19.496099393238946 27.748627564287776 34.10054897428489 18.654724068188386 56 21.03167494033137 26.802628309225092 32.61403498633256 19.551661764110982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 6.0 7 4.0 8 17.5 9 31.0 10 168.0 11 305.0 12 373.0 13 496.0 14 551.0 15 568.0 16 585.0 17 984.0 18 1383.0 19 1720.5 20 2058.0 21 2232.0 22 2797.0 23 3188.0 24 3838.5 25 4489.0 26 5118.0 27 5747.0 28 6178.5 29 6824.5 30 7039.0 31 7424.5 32 7810.0 33 6829.5 34 5849.0 35 5678.5 36 5508.0 37 4393.5 38 3029.0 39 2779.0 40 2940.5 41 3102.0 42 3172.0 43 3242.0 44 3571.5 45 3901.0 46 4941.0 47 5277.0 48 4573.0 49 4772.5 50 4972.0 51 5646.0 52 6320.0 53 6936.5 54 7751.0 55 7949.0 56 8240.5 57 8532.0 58 8588.5 59 8645.0 60 8976.5 61 9308.0 62 9044.5 63 8500.0 64 8219.0 65 7216.0 66 6213.0 67 5526.5 68 4840.0 69 4208.5 70 3577.0 71 3122.5 72 2305.0 73 1942.0 74 1818.0 75 1694.0 76 1359.0 77 1024.0 78 785.0 79 467.0 80 388.0 81 317.0 82 246.0 83 186.5 84 127.0 85 97.0 86 67.0 87 45.0 88 15.5 89 8.0 90 6.0 91 4.0 92 2.5 93 1.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.04813257085229031 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0011460135917211978 24 5.730067958605989E-4 25 0.04526753687298731 26 0.06245774074880528 27 0.1060062572342108 28 0.1060062572342108 29 0.14210568537342852 30 0.222899643589773 31 0.22232663679391237 32 0.2945254930723478 33 0.32374883966123835 34 0.33348995519086855 35 0.3592752610045955 36 0.40454279787758285 37 1.3310947867841711 38 0.4767416541560183 39 0.4670005386263881 40 0.6750020055237855 41 0.5306042929669146 42 0.5667037211061323 43 0.6125442647749803 44 0.651508726893501 45 0.6503627133017797 46 0.6933382229913246 47 0.6692719375651796 48 0.8136696501220505 49 0.7735591744118084 50 0.7689751200449237 51 0.7500658957815239 52 0.7878843443083235 53 0.8102316093468868 54 0.8308598539978683 55 0.8411739763233592 56 0.8474770510778258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 174518.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.186433491101205 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1515446999786 35.73098476947937 2 11.294503769591172 10.884837094167937 3 4.923062287440246 7.116744404588639 4 2.692225366851381 5.189149543313584 5 1.6933431636026353 4.079808386527464 6 1.174875734297334 3.39678428586163 7 0.8442932908412016 2.8478437754271764 8 0.6183556496301758 2.3837082707800916 9 0.4423621185815873 1.918426752541285 >10 2.0156015886983614 16.538122142128607 >50 0.09988822032487454 3.205973022840051 >100 0.045187528242205156 3.7308472478483594 >500 0.0023782909601160604 0.9145188461935159 >1k 0.0023782909601160604 2.0622514583022955 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1826 1.0463104092414535 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1773 1.0159410490608418 No Hit GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 941 0.5391993949048236 No Hit GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.37531945128869226 No Hit GTGGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 440 0.2521229901786635 No Hit GTGGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.16387994361613129 No Hit GTGGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.1610149096368283 No Hit GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 270 0.1547118348823617 No Hit GTGGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.14554372614859212 No Hit GTGGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 232 0.13293757663965894 No Hit GTGGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 227 0.13007254266035595 No Hit GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 217 0.12434247470174996 No Hit GTGGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 217 0.12434247470174996 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 195 0.11173632519281679 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 187 0.107152270825932 No Hit GTGGTAGGGGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTCTT 183 0.10486024364248961 No Hit GTGGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 176 0.10084919607146542 No Hit GTGGTAGGGGTCAGATGAATACATCCACTGGGATTCATTAGAGATGCTCAGTGAAT 175 0.10027618927560482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.012033142713072577 0.0 0.0 0.0 0.0 7 0.012606149508933176 0.0 0.0 0.0 0.0 8 0.012606149508933176 0.0 0.0 0.0 0.0 9 0.013752163100654373 0.0 0.0 0.0 0.0 10 0.025785305813726952 0.0 0.0 0.0 0.0 11 0.03953746891438133 0.0 0.0 0.0 0.0 12 0.04125648930196312 0.0 0.0 0.0 0.0 13 0.042975509689544916 0.0 0.0 0.0 0.0 14 0.0504245980357327 0.0 0.0 0.0 0.0 15 0.0509976048315933 0.0 0.0 0.0 0.0 16 0.0509976048315933 0.0 0.0 0.0 0.0 17 0.0532896320150357 0.0 0.0 0.0 0.0 18 0.060165713565362884 0.0 0.0 0.0 0.0 19 0.06245774074880528 0.0 0.0 0.0 0.0 20 0.07907493782876265 0.0 0.0 0.0 0.0 21 0.08251297860392624 0.0 0.0 0.0 0.0 22 0.09053507374597462 0.0 0.0 0.0 0.0 23 0.09913017568388362 0.0 0.0 0.0 0.0 24 0.1025682164590472 0.0 0.0 0.0 0.0 25 0.1048602436424896 0.0 0.0 0.0 0.0 26 0.1082982844176532 0.0 0.0 0.0 0.0 27 0.11059031160109559 0.0 0.0 0.0 0.0 28 0.13007254266035595 0.0 0.0 0.0 0.0 29 0.13522960382310134 0.0 0.0 0.0 0.0 30 0.1461167329444527 0.0 0.0 0.0 0.0 31 0.1512737941071981 0.0 0.0 0.0 0.0 32 0.1564308552699435 0.0 0.0 0.0 0.0 33 0.1598688960451071 0.0 0.0 0.0 0.0 34 0.1644529504119919 0.0 0.0 0.0 0.0 35 0.19482231059260363 0.0 0.0 0.0 0.0 36 0.19997937175534902 0.0 0.0 0.0 0.0 37 0.2039904193263732 0.0 0.0 0.0 0.0 38 0.2091474804891186 0.0 0.0 0.0 0.0 39 0.2177425824270276 0.0 0.0 0.0 0.0 40 0.22289964358977296 0.0 0.0 0.0 0.0 41 0.22977572514010017 0.0 0.0 0.0 0.0 42 0.23550579309870615 0.0 0.0 0.0 0.0 43 0.24410089503661514 0.0 0.0 0.0 0.0 44 0.25154998338280293 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACATA 20 3.9080603E-4 50.67124 37 CGAAGAA 25 1.9222036E-5 50.67124 37 TTCATTA 30 9.548476E-7 50.64167 34 GCGAAGA 25 1.928858E-5 50.641666 36 AGGATTT 25 1.928858E-5 50.641666 35 AATTGTG 15 0.008079185 50.523727 33 ATGCGTT 30 9.703399E-7 50.523727 33 CTATTTA 25 1.9556668E-5 50.523724 33 TATGCGT 30 9.742471E-7 50.494328 32 TGCGATC 15 0.008097803 50.494328 32 ACCGTCT 15 0.008097803 50.494328 32 GTATGCG 40 2.4447218E-9 50.47964 31 CCTAATC 15 0.008276263 50.21673 40 CAAGCTA 15 0.008276263 50.21673 40 CATAACT 15 0.008276263 50.21673 40 GAAATAG 15 0.008285736 50.2022 38 TTAGAGA 30 1.0140539E-6 50.2022 38 TCCCTAA 15 0.008285736 50.2022 38 ACATAAC 15 0.008285736 50.2022 39 CGCTAGT 25 2.0309875E-5 50.202198 39 >>END_MODULE