FastQCFastQC Report
Fri 17 Jun 2016
SRR1526601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260701
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC12180.4672018902881078No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.45492729218530037No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.3528946954557136No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7050.2704247394524762No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC6760.259300884921807No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5260.20176370631489715No Hit
ATACAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT4920.18872194583066426No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4610.17683092891856955No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4220.161871262480773No Hit
ATACAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.16110410009934753No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3730.1430757841358491No Hit
ATACAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3560.13655490389373268No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3410.1308011860330417No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3370.12926686127019074No Hit
ATACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.12773253650733984No Hit
ATACAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCATTGACTGCTCTTACAGAGGTCCT3270.12543104936306343No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3050.11699226316738331No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC3000.11507435721381967No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.113156451260256No Hit
ATACAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2820.10816989578099048No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2760.1058684086367141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAG150.004627110458.1766354
TAATGTC150.004627110458.1766355
CCAGGTA201.9812846E-458.154250
ATAGAAC150.00463418158.154250
TATAGAG201.9812846E-458.154251
AGCTCGA150.00463418158.154252
GAACATA201.986956E-458.12059446
AATCCGG201.9888494E-458.10939843
AGCGCAT150.00464834658.10939843
TAATCCG201.9888494E-458.10939842
TAGCTTA150.004651892558.0982141
TACGACC150.00468746657.98654629
GTAATTC202.0116822E-457.97540328
CAATTCT150.00469817857.9531327
CATTGCA150.00471248857.90863424
CGTAAGA150.00471248857.90863422
GCGTAAG150.00471248857.90863421
CATTAGT150.00472683157.86421210
TTAGTAG150.00472683157.86421212
TACAGGT258.771885E-657.8642122
Produced by FastQC (version 0.11.5)