FastQCFastQC Report
Fri 17 Jun 2016
SRR1526600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251796
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.41223847876852693No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10210.4054869815247264No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.311363167008213No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5600.22240226214872358No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5560.22081367456194698No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4190.16640454971484853No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3920.1556815835041065No Hit
ATACAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3610.14337002970658788No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3530.14019285453303468No Hit
ATACAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.12788130073551604No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2980.11834977521485647No Hit
ATACAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2700.10722966210742028No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2590.10286104624378466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCTA303.625173E-758.28413836
ACGGCTA150.00460781458.23742739
TCACGCG150.004633459358.1558657
ACTTACG150.004637131558.14422654
ACACTCG150.004637131558.14422655
CTTACGG150.004637131558.14422655
CGCCAGT150.004644482458.12097551
TCCCGTA150.00465184258.0977446
ACCGGAC201.9965913E-458.06292341
ACGTATG150.00466289858.06292329
ACGTACT201.9965913E-458.06292341
TACGTAC201.9985643E-458.05132740
AGCAATG150.004725917757.86641311
CGTAGAC202.0303423E-457.86641313
TACGAAT150.004725917757.86641311
ACGGAAA150.004725917757.86641311
CGAATCA150.004725917757.86641313
TACTACT303.8521648E-757.774420
GGTCTAA150.004755792757.77448
GGATTAC202.0463811E-457.77448
Produced by FastQC (version 0.11.5)