FastQCFastQC Report
Fri 17 Jun 2016
SRR1526599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231738
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17660.7620675072711424No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13060.5635674770646161No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.3827598408547584No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8360.3607522288101218No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5600.241652210686206No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC5470.23604242722384763No Hit
ATACAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.18296524523384167No Hit
ATACAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3980.17174567830912496No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3770.16268372040839224No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3450.14887502265489477No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3350.1445598046069268No Hit
ATACAGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3340.14412828280213No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2960.12773045421985174No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2890.12470980158627415No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.12212067075749339No Hit
ATACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.12168914895269657No Hit
ATACAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2770.11953153992871259No Hit
ATACAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCATTGACTGCTCTTACA2550.11003806022318308No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2510.10831197300399588No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2500.10788045119919909No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2450.10572284217521512No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2390.10313371134643434No Hit
ATACAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10227066773684074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTT150.00800416750.6484236
CACTAAT204.0794344E-450.23969339
CGCATTC150.00827900350.21779345
AAAACAT150.00827900350.21779347
ATGCGAG150.00827900350.21779346
ACATATA150.00827900350.21779348
AATTTCT204.0882253E-450.2177945
CATTATG355.0873496E-850.2177946
TACTATA150.00830043150.1849750
ACTCAAC252.0372245E-550.18496749
TAATCCG204.1014398E-450.18496742
TAGGTAA150.00842986749.98893430
TCGGGAT150.00843710249.9780929
ACGTATG150.00843710249.9780929
AATTGGG355.3465556E-849.90229426
ACCCATA204.2672284E-449.7836623
GCGTTTA150.00857543949.77290721
GACCTCG204.2854657E-449.7406620
ACCGCGT204.2991844E-449.7084618
GAACGTA204.2991844E-449.708469
Produced by FastQC (version 0.11.5)