FastQCFastQC Report
Fri 17 Jun 2016
SRR1526598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178494
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9470.5305500465001625No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.4117785471780564No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4560.2554707721267942No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.2151332817909846No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.21065133842033903No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.20672963797102425No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3690.20672963797102425No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3460.1938440507804184No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.1932838078590877No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2970.16639214763521465No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2590.14510291662464844No Hit
TAAGGGGGGATTCAATCACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGACGCTA2170.12157271392875951No Hit
TAAGGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2110.11821125640077539No Hit
TAAGGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2080.1165305276367833No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2040.11428955595146055No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1990.1114883413448071No Hit
TAAGGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.1064461550528309No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.10476542628883884No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1870.10476542628883884No Hit
TAAGGGGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGCGGACCGCGCCGG1830.10252445460351609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA201.9716265E-458.20152358
ACGATCG150.0046168858.20152358
TGTAATT150.0046168858.20152358
ACCCACG258.463754E-658.20152357
AGTAATA150.0046168858.20152358
TGCGATG150.00462719658.1687350
ACGAAGG150.00464270258.11961741
CTGTCGT201.9854061E-458.11961439
TCTAGAT201.9854061E-458.11961440
TACTCGG201.9854061E-458.11961444
TGTCGTA201.9854061E-458.11961440
TACTCTG150.004647879458.10326438
CGGTATT201.9881711E-458.10326436
TTCGCTT150.004647879458.10326437
ATGCGTT150.004668631658.0379433
CTATCGC150.004673830258.02163732
TCTATCG150.004673830258.02163731
GCGTGTA150.004694668657.95649329
ACAGTAG150.00470511457.92398527
CTTAACC202.024397E-457.89150221
Produced by FastQC (version 0.11.5)