FastQCFastQC Report
Fri 17 Jun 2016
SRR1526597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172745
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8140.47121479637616137No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.3351761266606848No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4060.23502851023184465No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3150.18234970621436222No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2900.16787750730846046No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.16382529161480794No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.16208862774609975No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1609308518336276No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.1528264204463226No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2290.13256534197806016No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2280.13198645402182407No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2030.11751425511592233No Hit
TAAGGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1990.11519870329097803No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1830.10593649599120089No Hit
TAAGGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1810.10477872007872875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGA201.9641122E-458.2453358
GACGCGT201.9641122E-458.2453358
TATTTGC150.00460287458.24532756
TCTTCGT150.00460287458.24532758
TTAAATC150.00460287458.24532758
TAAGTAT201.966951E-458.22832537
ATAGCCG258.439449E-658.2283254
CCTATCT150.00460820158.2283254
AGGTAGC150.00461353258.21132739
ATACTGT150.00461353258.21132736
AGTATAC201.9697928E-458.21132739
CGCAGCA201.9697928E-458.21132738
TATGTAC258.454073E-658.21132738
GACTTCG201.9726381E-458.19434433
CTGATCC201.9726381E-458.19434451
AGTTCGA150.004618867858.1943434
GCGTTGC303.6793972E-758.1434435
TTCAAGC150.004645615458.1095646
TTCGTCA201.9926461E-458.07571844
GATAGGG150.004661719358.05880731
Produced by FastQC (version 0.11.5)