FastQCFastQC Report
Fri 17 Jun 2016
SRR1526596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158578
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10450.6589816998574834No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9610.6060109220698962No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7120.44899040219954844No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3440.21692794713011893No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.21629734263264766No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.18981195373885407No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3010.18981195373885407No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2720.17152442331218706No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.16900200532230195No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.15512870637793388No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2350.14819205690574985No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2340.14756145240827856No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2280.1437778254234509No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCC2210.139363593941152No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1970.12422908600184138No Hit
TAAGGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1860.11729243652965733No Hit
TAAGGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1710.10783336906758818No Hit
TAAGGGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1700.1072027645701169No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1650.10404974208276055No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.10278853308781798No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.10026611509793289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAAC150.0079727150.6913237
CAGGACC251.9168812E-550.6913236
CAGTACG203.899312E-450.69131537
TACCAAG150.00798285350.67502635
GTTCGAG150.00798285350.67502635
TAAAGGC203.9179192E-450.64246734
CTTTGAC150.00807457150.52884733
CGAATAT150.00807457150.52884733
GCGAATA150.0080848150.51265732
ACATTAC150.0080848150.51265732
CGCGATC150.00827077250.22339
GTACGTA204.082157E-450.22339
TACGTAG204.082157E-450.22340
TCCTCGA150.00828119650.20700538
GTCTACC150.00831252550.1590850
TTGCGTA150.00831252550.1590848
AGTCTAC150.00831252550.1590849
GAACCGC252.0404796E-550.1590848
CGACTGA150.00831252550.1590850
CTAGCTT150.00831252550.1590850
Produced by FastQC (version 0.11.5)