##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158578
Sequences flagged as poor quality	0
Sequence length	56
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.438591734036244	39.0	38.0	40.0	35.0	40.0
2	37.94863095763599	39.0	38.0	40.0	35.0	40.0
3	38.069574594206	39.0	38.0	40.0	35.0	40.0
4	38.010215792859036	39.0	38.0	40.0	35.0	40.0
5	37.888900099635514	39.0	38.0	40.0	35.0	40.0
6	38.07524372863827	39.0	38.0	40.0	35.0	40.0
7	38.194894625988475	39.0	38.0	40.0	36.0	40.0
8	38.37551867219917	39.0	38.0	40.0	36.0	40.0
9	37.627899204177126	39.0	37.0	40.0	35.0	40.0
10	36.56584772162595	39.0	35.0	40.0	31.0	40.0
11	36.79031769854583	39.0	35.0	40.0	32.0	40.0
12	37.01654075596867	39.0	35.0	40.0	32.0	40.0
13	37.087918879037446	39.0	36.0	40.0	32.0	40.0
14	37.09135567354866	39.0	36.0	40.0	32.0	40.0
15	37.175320662386966	39.0	36.0	40.0	33.0	40.0
16	37.030659990667054	39.0	36.0	40.0	33.0	40.0
17	36.802507283481944	39.0	36.0	40.0	32.0	40.0
18	36.780776652499085	39.0	36.0	40.0	32.0	40.0
19	36.554900427549846	38.0	36.0	40.0	31.0	40.0
20	36.420947420197	38.0	36.0	40.0	31.0	40.0
21	36.299751541827995	38.0	35.0	40.0	30.0	40.0
22	36.21213535294934	38.0	35.0	40.0	30.0	40.0
23	36.030956374780864	38.0	35.0	40.0	30.0	40.0
24	35.81347980173795	38.0	35.0	40.0	29.0	40.0
25	35.666883174210795	38.0	35.0	40.0	29.0	40.0
26	35.06955567607108	38.0	34.0	39.0	28.0	40.0
27	34.88310484430375	38.0	34.0	39.0	27.0	40.0
28	34.637843837102245	38.0	33.0	39.0	27.0	40.0
29	34.482456582880346	38.0	33.0	39.0	26.0	40.0
30	34.06442255546167	38.0	33.0	39.0	24.0	40.0
31	33.30444954533416	37.0	32.0	39.0	21.0	40.0
32	33.16157348434209	36.0	32.0	39.0	21.0	40.0
33	32.91353781735172	36.0	32.0	39.0	20.0	40.0
34	32.72126020002775	36.0	31.0	39.0	18.0	40.0
35	32.50652675654883	36.0	31.0	39.0	17.0	40.0
36	32.13423047333173	36.0	31.0	39.0	7.0	40.0
37	31.65821236237057	36.0	31.0	39.0	4.0	40.0
38	31.656043082899266	36.0	30.0	39.0	4.0	40.0
39	31.474372233222766	36.0	30.0	39.0	4.0	40.0
40	31.042515355219514	36.0	30.0	39.0	4.0	40.0
41	30.395855667242618	35.0	29.0	39.0	4.0	40.0
42	30.2579298515557	35.0	29.0	39.0	4.0	40.0
43	30.086235165029198	35.0	28.0	38.0	4.0	40.0
44	29.699176430526304	35.0	28.0	38.0	4.0	40.0
45	29.37532949084993	35.0	27.0	38.0	4.0	40.0
46	28.69276318278702	34.0	26.0	38.0	4.0	40.0
47	28.37945364426339	34.0	25.0	38.0	4.0	40.0
48	28.043751340034557	34.0	23.0	38.0	4.0	40.0
49	27.84114442104201	34.0	23.0	38.0	4.0	40.0
50	27.413140536518306	33.0	21.0	38.0	4.0	39.0
51	26.688594887058734	33.0	4.0	38.0	4.0	39.0
52	26.373191741603502	33.0	4.0	37.0	4.0	39.0
53	26.02186305792733	33.0	4.0	37.0	4.0	39.0
54	25.654012536417408	33.0	4.0	37.0	4.0	39.0
55	25.123976844202854	32.0	4.0	37.0	4.0	39.0
56	15.000876540251484	18.0	4.0	22.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.5628072696580162
1	2	-0.5715530186911053
1	3	-1.0174301238031802
1	4	-0.9792447516023728
1	5	-0.8278427700956357
1	6	-1.0938950426787457
1	7	-1.326156602533196
1	8	-1.6042027936475023
1	9	-1.3574323148845409
1	10	-0.6993781670626973
1	11	-0.5533231824022735
1	12	-0.7974444265632386
1	13	-1.2771908073954776
1	14	-1.1615261664403107
1	15	-1.7609632430855768
1	16	-1.6465009652327893
1	17	-1.3788220935489832
1	18	-1.301278328463475
1	19	-1.4006676298490177
1	20	-1.2484678115033034
1	21	-1.3110387592016508
1	22	-1.3433015702209445
1	23	-0.8278425786612615
1	24	-1.0323587931781617
1	25	-0.8893403291866733
1	26	-0.6152227209310084
1	27	0.13164375545528628
1	28	-0.00789427236978213
1	29	-0.7535850314716654
1	30	-0.143463040279471
1	31	0.7454979371701071
1	32	0.056801980087023196
1	33	0.6200365063289297
1	34	0.7552981505211065
1	35	0.7922022962071651
1	36	1.026481751848415
1	37	0.8692129982090258
1	38	1.3738945406745202
1	39	0.6880661214158899
1	40	0.5094168500113092
1	41	0.6709493777974096
1	42	0.5528378057321284
1	43	0.7333852322742658
1	44	0.5022611109746684
1	45	0.6598561918373527
1	46	0.9644854673138532
1	47	1.154736597176658
1	48	1.383425086297116
1	49	1.708593836064118
1	50	0.9819931075778854
1	51	1.588881960611502
1	52	1.7050781998847562
1	53	1.692073543272194
1	54	1.6366488378284743
1	55	2.2559071903115786
1	56	2.7187950262147798
2	1	-0.36740020615279434
2	2	-0.007052935786120429
2	3	-0.7510563881042884
2	4	-0.32877219315435013
2	5	0.0063057956480250255
2	6	-0.3440194001650667
2	7	0.1686203830413362
2	8	-0.0810723011918526
2	9	-0.28451737540517996
2	10	-0.5547504104717476
2	11	-1.025259842989243
2	12	-1.0338480080588468
2	13	-1.2020788856577909
2	14	-0.8797735549330952
2	15	-1.4191459656854803
2	16	-1.1887410580863786
2	17	-1.4805879698547315
2	18	-1.5086651631509298
2	19	-1.2401635675044602
2	20	-1.6800297415314915
2	21	-1.0901798634961324
2	22	-0.8590949709836693
2	23	-1.168540638684476
2	24	-1.454179386777895
2	25	-1.032683058418975
2	26	-1.951982793887403
2	27	-1.9867860240174409
2	28	-2.761998069418368
2	29	-2.1907015958888465
2	30	-1.7248757413240767
2	31	-1.7575695474924693
2	32	-2.3606246489960476
2	33	-1.1898208970867543
2	34	-1.6612165236622793
2	35	-1.9841614908928165
2	36	-2.498721365379243
2	37	-1.9620753453492554
2	38	-2.484213567433585
2	39	-2.476500285481805
2	40	-1.358681309497893
2	41	-1.9684139118726272
2	42	-2.3770245719830108
2	43	-2.428362404933491
2	44	-2.108375599355295
2	45	-2.174499636383505
2	46	-1.9663717702918504
2	47	-2.566122298528864
2	48	-1.9298728742401074
2	49	-1.8705389777312718
2	50	-1.1404990164479791
2	51	-1.6450344711576896
2	52	-1.7808279516655645
2	53	-1.3142686885301593
2	54	-0.9666922333040056
2	55	-0.6512392199023154
2	56	-1.2275239921901289
3	1	-0.18684521488801664
3	2	0.032438389936061185
3	3	-0.35992162192381727
3	4	0.043307602075813634
3	5	-0.24510605987548217
3	6	-0.4088225529704985
3	7	-0.24847627319724097
3	8	-0.49126203832263826
3	9	-0.8820877650366796
3	10	-0.5065644924609458
3	11	-0.9664697727011813
3	12	-0.5822573852172539
3	13	-0.5709083660136969
3	14	-0.7417570595851117
3	15	-0.7361735527329714
3	16	-0.7741633958753553
3	17	-0.5332430708176403
3	18	-0.7751909828397103
3	19	-0.6891945673825717
3	20	-0.5707785328206469
3	21	-0.6951447194665548
3	22	-1.0824728607167344
3	23	-1.0629866631430929
3	24	-1.2466061531208865
3	25	-0.8461149846368272
3	26	-1.6442186042315257
3	27	-1.941606647265317
3	28	-1.9203696616663635
3	29	-1.5963002632597423
3	30	-1.6636885638162582
3	31	-1.9101312019027397
3	32	-2.304841119966671
3	33	-1.9005022443422703
3	34	-2.0485321698655063
3	35	-1.7952775677627635
3	36	-1.921343556904045
3	37	-1.3195287236452806
3	38	-0.6563989978971598
3	39	-1.022959473715666
3	40	-0.9574705672072348
3	41	-0.8538403125551959
3	42	-0.8346689062778943
3	43	-1.1902768403944286
3	44	-0.7461280066197489
3	45	-0.7176272518712565
3	46	0.01782357363209286
3	47	-0.3121508200418859
3	48	0.21777267892285224
3	49	0.2550856921586231
3	50	-0.044452211913792894
3	51	-0.7715529776380059
3	52	-0.5865810089929937
3	53	-0.9039976935275185
3	54	-1.520098969581582
3	55	-1.3730528956021466
3	56	-1.4418010002752872
4	1	0.2509964669645157
4	2	0.6839340119637072
4	3	-0.08390935471194183
4	4	0.5317293097072593
4	5	0.8522315995760508
4	6	0.34334420999674364
4	7	0.6553416742670919
4	8	0.3914438597172776
4	9	0.5011292668314056
4	10	0.3635186223441593
4	11	0.13709038552807584
4	12	-0.20126267434121559
4	13	0.28801869741134567
4	14	0.33597972871659465
4	15	-0.3073989405279889
4	16	-0.17955919124403863
4	17	-0.30123275924823645
4	18	-0.49268953833106366
4	19	0.0544384829752218
4	20	-0.19627299924137986
4	21	-0.24392466524192002
4	22	0.30856674104334303
4	23	-0.17796955127145253
4	24	0.07647487270248376
4	25	-0.1700404924371739
4	26	0.012566141757183402
4	27	0.07110502880917124
4	28	-0.09913316226639068
4	29	0.04675053899813264
4	30	0.5420983600470279
4	31	1.0475113599888957
4	32	1.1359784720151964
4	33	0.8177963467062241
4	34	1.0273823152227237
4	35	0.7235010446200079
4	36	1.2543975871467978
4	37	1.5101525955244632
4	38	1.6768602580697731
4	39	1.5127532246264828
4	40	1.855054433903927
4	41	1.6234253436961943
4	42	1.5807717799748282
4	43	0.989597820701622
4	44	1.783006621585951
4	45	1.693413238817218
4	46	1.9467093018866812
4	47	1.3325889461699454
4	48	2.105989933023036
4	49	2.242196230404769
4	50	1.9260798117317037
4	51	2.1382652365048465
4	52	2.1613633440893665
4	53	2.305033818984146
4	54	2.245890629956172
4	55	0.7705090404839012
4	56	1.3513453617852509
5	1	0.04294277568934746
5	2	0.45574609250061826
5	3	0.016761786227661446
5	4	0.26327293386833617
5	5	0.4227013982337695
5	6	-0.24725981684888865
5	7	0.5815161709113852
5	8	0.38473245032130166
5	9	0.11857893126093444
5	10	0.44405553509583484
5	11	-0.05082907755917887
5	12	0.08061652028965227
5	13	0.05311936855228083
5	14	0.22859717838102256
5	15	0.424144683633088
5	16	0.29023946647408394
5	17	-0.032776383409313326
5	18	0.20529703885014072
5	19	-0.039521248568405554
5	20	0.14600887995324996
5	21	-0.6130521820204393
5	22	0.10051304976817477
5	23	0.30525192523860767
5	24	0.009360778742752984
5	25	-0.055946532705625884
5	26	0.4152507055155752
5	27	0.2791587200843395
5	28	1.0216722068611261
5	29	0.31520691483706287
5	30	0.9112258768255472
5	31	0.9535516284452754
5	32	0.6259113579212325
5	33	0.9184674876458203
5	34	1.007248087034803
5	35	0.7100782258280631
5	36	0.858424432784382
5	37	-1.5838071360191606
5	38	0.0929676406201061
5	39	1.0765116138882256
5	40	0.7074034271925171
5	41	1.220740779937806
5	42	0.5002348672231491
5	43	0.935906545533836
5	44	1.225959641720177
5	45	1.4585139099581568
5	46	1.242011315309501
5	47	1.6748708253645788
5	48	1.5757885907411548
5	49	1.1079680424853038
5	50	1.134133503006872
5	51	0.9839028203974642
5	52	1.0070009279819807
5	53	0.9963089867693817
5	54	0.5613268715669122
5	55	1.085945282094638
5	56	0.7070500597718272
6	1	-0.08024406128835437
6	2	0.21214358113452647
6	3	-0.22606133566019793
6	4	-0.32798058420917187
6	5	0.006464117437054995
6	6	-0.4807302649978453
6	7	0.381602769742301
6	8	0.08263242867159448
6	9	-0.25111147791854194
6	10	-0.25678880350559297
6	11	-0.5397660769096859
6	12	0.09170117064687133
6	13	-0.3319423331147462
6	14	-0.013792949352257722
6	15	-0.25730151684320646
6	16	-0.4270153505858829
6	17	-0.6309145085446204
6	18	-1.111871179378909
6	19	-1.0801793996833666
6	20	-4.730425407970529E-4
6	21	-0.601361339846811
6	22	-0.3504070628103051
6	23	-0.7257937556446947
6	24	-0.5967877382522673
6	25	-0.1072130406077676
6	26	0.46080169057727716
6	27	0.021050904539848148
6	28	0.313640165293684
6	29	-0.08423019276198573
6	30	-0.19481439163082825
6	31	-0.525469804765379
6	32	-0.5588039484221312
6	33	-0.9008440516483986
6	34	-0.659000018615771
6	35	0.026683551656162763
6	36	-0.3688974983695452
6	37	-0.17917409856516642
6	38	0.03836707772770609
6	39	-0.7367813066573454
6	40	-0.009937988698368372
6	41	0.6027715225227794
6	42	0.48919351628138585
6	43	0.5864802627886512
6	44	0.37205186947510427
6	45	-0.2562728404207135
6	46	0.2126110122135927
6	47	0.11280111330568943
6	48	0.039135635556050374
6	49	0.0927699476595869
6	50	-0.7168299089870658
6	51	-0.7852679957965165
6	52	0.22704855733898555
6	53	-0.7510389240338213
6	54	-0.7605176835315959
6	55	0.3315828659872544
6	56	-1.5989469092690918
7	1	0.1187860884059333
7	2	0.4045112809026037
7	3	0.23402721588945496
7	4	0.018635481571976698
7	5	0.2548722979135505
7	6	-0.12171698226539718
7	7	0.03473369953357519
7	8	0.06087404789992945
7	9	-0.01345598561409389
7	10	0.12322384671578845
7	11	-0.39411986143424826
7	12	-0.10361824744294523
7	13	-0.6426739375844903
7	14	-0.34687327825800907
7	15	-0.7158298744227949
7	16	-0.647340039601275
7	17	-0.8114753350257473
7	18	-0.4293917359886521
7	19	-0.9950855772081368
7	20	-0.5237283661756251
7	21	-0.46136555658727474
7	22	-0.8162303813850471
7	23	-1.1209445041161317
7	24	-0.5743125116024643
7	25	-0.7136646535109961
7	26	-0.6040939450963521
7	27	-0.5879729200437467
7	28	-0.6743842918009832
7	29	-0.7323432014063087
7	30	-0.890239228232133
7	31	-1.2610380105423111
7	32	-1.3550510494130634
7	33	-1.0640740748404731
7	34	-0.4819390867161317
7	35	-0.4965717719786653
7	36	-0.027130684116961845
7	37	-0.19287850505894966
7	38	-0.27506324063620013
7	39	-0.453163343342986
7	40	0.13359985654725648
7	41	0.5049009764548913
7	42	0.8587908175674919
7	43	1.0332439144858903
7	44	0.6862833685263361
7	45	1.221947695105328
7	46	0.891000217358016
7	47	1.0207496690720816
7	48	0.9606199281978398
7	49	1.2807100699450586
7	50	1.82084666701968
7	51	0.9045064085888903
7	52	0.7503882157830084
7	53	0.5983180230460867
7	54	0.5640026622411227
7	55	0.7551122777519623
7	56	0.014546660201707695
8	1	-0.03271717956792486
8	2	0.529347882209791
8	3	-0.042835529208581136
8	4	-0.2374429498005668
8	5	0.28323831098544616
8	6	-0.10524639403009672
8	7	0.39041997404338247
8	8	-0.0061847756294781675
8	9	0.06824336079113635
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113	8	0.09814193518729297
113	9	0.4225479965527228
113	10	0.6589984214463414
113	11	0.3116759226630563
113	12	0.1727095021999645
113	13	0.29001878630418076
113	14	0.23837539074601466
113	15	0.1578661823663623
113	16	-0.07217664090846654
113	17	0.0429158695797085
113	18	0.2645441164980369
113	19	0.2313352339419268
113	20	-0.05422224142991894
113	21	-0.01964803072483079
113	22	-0.09571789382932394
113	23	-0.1490793783748856
113	24	-0.2333611200083041
113	25	-0.40902889192159364
113	26	-0.008457014827556009
113	27	0.223956266641963
113	28	-0.34421072126901464
113	29	-0.6227903294849071
113	30	-0.2001124048758598
113	31	0.24880919544825275
113	32	0.5097284164571718
113	33	0.1988355040020977
113	34	0.428289022187478
113	35	0.20472243223724007
113	36	-0.33856209995040487
113	37	-2.213568458744618
113	38	-0.2193129051819298
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113	41	-0.6882773986479087
113	42	-0.5101655950196182
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113	45	-0.4262365233944365
113	46	0.05093615641354887
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113	49	0.4670062219599487
113	50	1.326408715238525
113	51	0.14897682368649967
113	52	0.9101077327288607
113	53	0.8244791276645316
113	54	1.3938083151866287
113	55	-0.2273575313637366
113	56	-0.5212711989507834
114	1	0.10304039618964822
114	2	0.7232872761491862
114	3	0.2784631720901203
114	4	0.03900726664964793
114	5	0.6071344148815498
114	6	0.2950133462296378
114	7	0.501502225125634
114	8	0.31857712913242153
114	9	0.5422693348171137
114	10	0.548866831182302
114	11	-0.2791041581832516
114	12	-0.16766204678086183
114	13	-0.33370186390653345
114	14	-0.24743350234764705
114	15	0.0993386172162829
114	16	-0.08516365389547786
114	17	0.0066640419371566395
114	18	-0.08778236496751646
114	19	0.39715616797478503
114	20	-0.21819403122952252
114	21	-0.22662317478606298
114	22	0.20066122374184658
114	23	0.029212528393841808
114	24	0.20333794251857285
114	25	-0.32405426390060654
114	26	-0.8668899725982584
114	27	-0.3626524888409648
114	28	-0.5310602955358092
114	29	-0.951636979523613
114	30	-1.9753338721503084
114	31	-0.8583981667259977
114	32	-0.9510955278104802
114	33	-1.511149874857452
114	34	-0.9639015686786117
114	35	-0.7040244500195776
114	36	-7.4491237237570616
114	37	-27.630787001790974
114	38	-7.704992788212806
114	39	-7.3036853703356
114	40	-6.802271461677002
114	41	-7.403638196790165
114	42	-6.713571460677137
114	43	-6.694835195946162
114	44	-6.692631833918277
114	45	-6.768715236734074
114	46	-0.5088385060101217
114	47	-0.2608478184077576
114	48	-0.09726989439786493
114	49	-0.7002338227635398
114	50	-0.26467238908761104
114	51	0.2327043544338956
114	52	0.20455169935825523
114	53	0.24393384513249572
114	54	-0.4759345247548872
114	55	-0.05543012102573286
114	56	-1.4078283504085967
115	1	0.14361391662894363
115	2	0.8450078374670653
115	3	0.2516611150867192
115	4	0.384078302995853
115	5	0.9663255593075917
115	6	0.04133078717795513
115	7	0.6888987212469573
115	8	0.3713096315293569
115	9	0.7158943675025427
115	10	0.2896931189884526
115	11	0.36527830499115765
115	12	-0.08045730521369876
115	13	-0.034128953595370604
115	14	-0.08012765383374187
115	15	0.0013258916867755488
115	16	-0.3204987885594761
115	17	-0.18713879951669554
115	18	-0.7141660483981767
115	19	-0.25428634923954263
115	20	-0.8472797106507741
115	21	-0.525803859872795
115	22	-0.9129097690237771
115	23	-0.7417279405332025
115	24	-0.5275519729351004
115	25	-0.65997337834321
115	26	-0.06797077099449211
115	27	-0.3517137631371341
115	28	-0.6024888669643786
115	29	-0.5975447630152928
115	30	-0.4780358681408927
115	31	-0.522958438668816
115	32	-0.4613369642264189
115	33	-1.0009955996025042
115	34	0.26899305347775737
115	35	-0.35703586813166766
115	36	-0.5375487215780304
115	37	0.7853203807593623
115	38	0.515786432566415
115	39	0.8147666474452784
115	40	0.2778732258502359
115	41	0.7240964846357905
115	42	0.32573822292784627
115	43	1.137248827413032
115	44	1.3266307826597767
115	45	1.619587735461515
115	46	2.067514671014198
115	47	1.3728574025457867
115	48	1.6160570471169962
115	49	1.463672740471882
115	50	0.6240663889129117
115	51	0.2523591962363909
115	52	0.07411502194171504
115	53	-0.2520131608816243
115	54	0.31300472391590617
115	55	0.0725224633026933
115	56	-0.9573794704295153
116	1	-0.1005710608464625
116	2	0.07135701462988209
116	3	-0.05982183057844992
116	4	-0.3441096164672359
116	5	-0.017309634220033843
116	6	-1.0205888597835227
116	7	-2.891132537372144
116	8	-0.4994267847619014
116	9	-0.9317566392088636
116	10	-1.5880305180438157
116	11	-1.3734823826984623
116	12	-0.7197880030164043
116	13	-1.232207467891591
116	14	-1.0194491579249458
116	15	-1.015498600360658
116	16	-0.024231435429015846
116	17	-0.1536277210943311
116	18	0.027766469759399115
116	19	-0.30903490122998534
116	20	-0.08787914064065916
116	21	0.30539959696272945
116	22	-0.5231423699247557
116	23	-0.09950122360757518
116	24	0.1770081079695629
116	25	0.06715726429722224
116	26	-0.252764372171292
116	27	0.4332912934041886
116	28	-0.033995716279449084
116	29	-0.750160373937419
116	30	-0.5975541176582198
116	31	-1.2134061724189351
116	32	-0.577407571930749
116	33	-1.1329253596466344
116	34	-0.48498322674657146
116	35	-0.5675718615121959
116	36	-1.0277574188291112
116	37	-0.3910609743937137
116	38	-0.42941904688564136
116	39	-0.460952760479703
116	40	-0.456336574646226
116	41	0.24740254329167044
116	42	-0.06582194991392853
116	43	0.3953622778129571
116	44	0.29909561671328433
116	45	0.3584863288236555
116	46	0.5761107912679435
116	47	0.7780242684095349
116	48	0.5250393032537986
116	49	0.3591862092592315
116	50	0.09611750594886104
116	51	0.04972979474700878
116	52	0.5340304397959024
116	53	0.9135655832573839
116	54	0.31093613882810445
116	55	0.6850075235215023
116	56	0.2975621495024505
117	1	0.48479916030135684
117	2	0.8554916730594684
117	3	0.21441937275220369
117	4	0.33889691948047584
117	5	0.7516043240573396
117	6	-0.03674966200395602
117	7	-1.8214754507932227
117	8	-0.2397795468713113
117	9	-0.00692003790167206
117	10	0.2800865918138271
117	11	-0.06732247581333439
117	12	-0.0055790317566675185
117	13	0.12856789248087352
117	14	-0.07236347433644141
117	15	-0.3106011162528617
117	16	0.2742285878497057
117	17	0.22120440353634052
117	18	0.5444644731616748
117	19	0.21406474958924804
117	20	0.39784026127535554
117	21	0.2641246394911576
117	22	-0.2672107735419118
117	23	0.29082020176622336
117	24	0.3145763772864214
117	25	-0.19078883651753387
117	26	0.25865115294286767
117	27	-0.33960690697185214
117	28	-0.36719474931732066
117	29	-0.3532582340860415
117	30	-0.4457947837876901
117	31	0.05922342736618447
117	32	0.23972019241700337
117	33	0.4784095852902439
117	34	-0.24664496906907374
117	35	0.3923171169102204
117	36	0.051300688432057484
117	37	0.2646378348103333
117	38	0.038662249559880735
117	39	0.01742489195112995
117	40	-0.6311060258056855
117	41	-0.42973954642092593
117	42	-0.4091830386416575
117	43	-0.40466458224613433
117	44	0.12419186525836423
117	45	0.03894115915761276
117	46	-0.6318755996354497
117	47	-0.8289235335422944
117	48	-0.5099683070962797
117	49	-0.5558912372444844
117	50	-0.8654278427842428
117	51	-1.291572022783658
117	52	-0.9947098234326788
117	53	-1.4866492972153544
117	54	-0.7170430893928632
117	55	-1.1664563496990183
117	56	-0.6390481110401218
118	1	0.3628236592841745
118	2	0.45627944953871236
118	3	0.14338963635432833
118	4	0.07642018485726965
118	5	0.569463476548357
118	6	0.3858794337844671
118	7	0.619073395676935
118	8	0.356420080882657
118	9	0.4953956786719331
118	10	0.13581961349932214
118	11	0.39776863789484196
118	12	0.07337175385558936
118	13	-0.29938518720575047
118	14	0.14566015895793782
118	15	0.27044896382331984
118	16	0.5172273326014647
118	17	0.347551631169118
118	18	0.5029547125245273
118	19	0.5492160286439187
118	20	0.3033936526098131
118	21	0.7088287904672228
118	22	0.4274609141177024
118	23	0.3873444626847231
118	24	0.24346007204467668
118	25	0.5446134921843679
118	26	0.5809469586823823
118	27	0.4954794454499165
118	28	0.5034713979362806
118	29	0.7811831804488634
118	30	0.13101342293871454
118	31	0.20907409611050554
118	32	0.6355562310267047
118	33	0.23857060333985203
118	34	0.4680241215252252
118	35	-0.007198097564085515
118	36	-0.4975024973013973
118	37	-0.20032342563203542
118	38	-7.698588243130189E-4
118	39	-0.8507740432520876
118	40	-0.3283314943595528
118	41	-0.6750323655353263
118	42	-0.3512251976686258
118	43	-0.27636955562621424
118	44	0.13466669536700593
118	45	0.7062138077313911
118	46	0.7529229113804341
118	47	0.6348690475077845
118	48	0.9727395663400706
118	49	0.9173373477877647
118	50	-0.3490879735031953
118	51	-0.1357913882340327
118	52	0.0491805804109795
118	53	-0.03644802465334607
118	54	0.06268248737205795
118	55	-0.3333177962643994
118	56	-0.22325795391767045
119	1	0.23342317130926915
119	2	0.2920671804554047
119	3	0.2613749971072039
119	4	0.1427324887959216
119	5	0.7071856794064999
119	6	0.2850167638646397
119	7	0.5552445354468887
119	8	0.43793803138806453
119	9	0.5287696765806089
119	10	0.3169802966641839
119	11	-0.023806823938549826
119	12	-0.2492580823242605
119	13	-0.4162377490739999
119	14	-0.1910425249040344
119	15	-0.1469874251623935
119	16	0.3291391485652184
119	17	0.05698871726183086
119	18	0.1455566644241486
119	19	-0.1095936611434638
119	20	-0.8464407844762789
119	21	-0.5478808644648083
119	22	-0.7280810865794081
119	23	0.06902797610403155
119	24	-0.3293004716987866
119	25	-0.4307699166688934
119	26	-0.627313759337838
119	27	-0.4149423042886724
119	28	-0.07617307749069369
119	29	-0.8193745051558778
119	30	-0.5606038653150378
119	31	-1.4255546944088415
119	32	-1.231427038405151
119	33	-1.3019051721916952
119	34	-1.9081086773594045
119	35	-1.6877052429108979
119	36	-0.9219535255342315
119	37	-0.8610501596857105
119	38	-1.0302661990125337
119	39	-1.361187079153101
119	40	-2.049793676304482
119	41	-1.5680264401257773
119	42	-1.0152631900004465
119	43	-1.2356759341553207
119	44	-1.2850788605758297
119	45	-1.2480385450047535
119	46	-1.7236368655453234
119	47	-1.818947613349657
119	48	-1.7767811725933527
119	49	-1.0938599334949153
119	50	-1.592509026134774
119	51	-0.7635362470698617
119	52	-1.1768563662603349
119	53	-0.49658221775198896
119	54	-0.34948202988134014
119	55	-0.31183818664432295
119	56	-1.2219944474694309
120	1	-0.19257533559629536
120	2	-0.15646772062708436
120	3	-0.14269368523694936
120	4	-0.45403869448141876
120	5	0.14862838190742877
120	6	-0.35659391176059785
120	7	0.0802072080859304
120	8	-0.2386670451330275
120	9	-0.39629564630106273
120	10	-0.3064799496963957
120	11	0.31120624063778735
120	12	-0.2358668915814519
120	13	-0.23827955571826465
120	14	-0.20336055401937614
120	15	-0.09477577902577394
120	16	-0.1714674210503091
120	17	-0.4137559635892316
120	18	-0.034314556180554234
120	19	-0.5118426346410416
120	20	-0.9395258908592581
120	21	-0.49543592232221556
120	22	-0.4135047521262507
120	23	-0.41520121389223874
120	24	-0.5253624351179695
120	25	0.03810839613439754
120	26	0.13099157847477727
120	27	0.40924582561054734
120	28	0.12091538835476712
120	29	0.6240745429964178
120	30	0.9980934097876144
120	31	1.433441199581452
120	32	1.8044538873134002
120	33	1.3557562686444413
120	34	1.9625053577283111
120	35	2.286212263621387
120	36	1.6029624879036177
120	37	1.3975817925352665
120	38	1.70727579426854
120	39	1.5806288969573892
120	40	0.8959416727063463
120	41	1.7742654598368937
120	42	1.6157091877524472
120	43	2.065089276744267
120	44	1.8439804899280894
120	45	1.7236859790713943
120	46	1.6917233434879009
120	47	2.030623121999355
120	48	1.9675730640621154
120	49	2.350102365870523
120	50	2.771479402383328
120	51	2.6881248204926607
120	52	2.1809263884989036
120	53	2.4274099435022123
120	54	2.6619028152101123
120	55	2.300377192228389
120	56	1.5645397070658422
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	141.0
5	175.0
6	34.0
7	75.0
8	102.0
9	121.0
10	108.0
11	248.0
12	380.0
13	444.0
14	689.0
15	860.0
16	1003.0
17	1131.0
18	1346.0
19	1658.0
20	1822.0
21	2124.0
22	2323.0
23	2519.0
24	2858.0
25	2733.0
26	2739.0
27	3270.0
28	3602.0
29	4028.0
30	4746.0
31	5595.0
32	6694.0
33	8243.0
34	10814.0
35	13640.0
36	17550.0
37	21601.0
38	21424.0
39	11738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.63084572091967	0.9735507154935266	0.29780682936678193	0.09779673422002373
8	98.01044281047812	1.5002080994841656	0.30773499476598265	0.18161409527172748
9	93.49216158609643	3.8429038075899555	1.6351574619430185	1.0297771443705936
10	51.28012712986669	29.145909268624905	7.457528787095309	12.116434814413097
11	34.212816405806606	24.663572500599074	19.626934379295992	21.496676714298328
12	33.56770800489349	15.957446808510639	26.771052731148075	23.703792455447793
13	25.687043599994958	16.16176266569133	29.005284465688806	29.145909268624905
14	23.186066163023874	19.256769539280356	34.904589539532594	22.652574758163173
15	29.034292272572486	20.10997742435899	28.508368121681443	22.34736218138708
16	31.038353365536203	21.56478200002522	24.329352116939297	23.067512517499274
17	29.665527374541234	21.249479751289584	23.140662639205942	25.944330234963235
18	30.820164209411143	21.804411709064308	22.474113685378803	24.901310396145746
19	29.859753559762385	24.463670874900682	22.154397205160866	23.522178360176067
20	28.988888748754555	23.3084034355333	23.556231003039514	24.146476812672628
21	27.10716492830027	21.727477960372813	26.649976667633595	24.515380443693324
22	26.856814942804174	19.90061673119853	28.739799972253405	24.502768353743896
23	29.690938786835417	22.116069795745915	24.668772898970218	23.52421851844845
24	29.347702707815714	22.973552447376054	23.592806063892848	24.085938780915388
25	28.141778285310505	22.590393751458983	22.018157614147547	27.249670349082972
26	26.244052652170076	20.139581256231306	25.55939775609879	28.056968335499832
27	24.72396007600962	18.43107051091849	26.124835070485663	30.72013434258622
28	25.33761814267405	24.623553403329776	24.204963728541752	25.83386472545442
29	29.773904256662874	20.675761020588606	25.488821523304285	24.061513199444235
30	26.256841998811737	20.997509702049125	29.492333168998954	23.25331513014019
31	28.997048469565108	20.49512397059846	27.66727972545079	22.840547834385646
32	31.487138619559925	18.943893137013074	28.75800871550639	20.810959527920613
33	30.023027481147828	19.07295409686725	29.161394807429524	21.74262361455539
34	24.495425614030648	23.15600997127564	33.45375631113417	18.894808103559544
35	24.012935153367632	26.453148039159846	31.4645707161797	18.06934609129282
36	25.862199939228198	25.837511394712852	28.767851716803406	19.532436949255548
37	27.838207474819775	23.318549005572883	29.327930875811646	19.515312643795696
38	27.342468183229123	23.364184039985556	29.1555014981914	20.13784627859392
39	27.86350449115072	20.68336437231576	29.321703216652097	22.131427919881418
40	25.170262327754187	20.928461260163374	29.735133385379974	24.166143026702464
41	23.00017748028701	22.111508328896328	31.271392713166502	23.61692147765016
42	22.44709045028595	19.967411014316326	32.23773474848151	25.34776378691622
43	22.506090443079735	19.799015378368775	33.02035223062478	24.674541947926713
44	24.2417318786771	18.870547019287525	30.82893826596346	26.05878283607192
45	24.619055778738204	19.89414161414999	31.218069544136217	24.268733062975585
46	23.737110783765935	21.448163762889216	29.884568496977703	24.930156956367146
47	22.4670386522189	21.232376708372215	32.1710371795116	24.129547459897292
48	23.921045103461427	22.232605448015004	31.82289183433457	22.023457614188995
49	25.05242289069351	22.073531841346092	29.808608791795344	23.065436476165058
50	21.575392513803905	22.011271865449256	30.92837218759332	25.484963433153517
51	21.746007546594505	23.586284000965556	30.989315343471684	23.67839310896825
52	22.63376316341381	23.547019682362137	30.717703957444915	23.101513196779134
53	23.432573985558832	21.786331740058984	32.504703549272854	22.276390725109326
54	23.175477192670115	22.40929842186248	30.898940702213974	23.51628368325343
55	23.372924031638647	21.97347338436888	29.071186957959256	25.582415626033217
56	23.310570222290046	22.201663360292994	28.059031486850806	26.428734930566154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	13.0
5	26.0
6	105.0
7	184.0
8	262.5
9	341.0
10	334.0
11	327.0
12	217.5
13	109.5
14	111.0
15	201.5
16	292.0
17	527.5
18	763.0
19	965.5
20	1168.0
21	1153.5
22	1267.0
23	1395.0
24	1692.5
25	1990.0
26	2521.5
27	3053.0
28	3603.0
29	4307.5
30	4462.0
31	4288.0
32	4114.0
33	3624.5
34	3135.0
35	3157.0
36	3179.0
37	2790.0
38	2306.0
39	2211.0
40	2566.0
41	2921.0
42	2826.0
43	2731.0
44	2802.5
45	2874.0
46	3699.0
47	4350.0
48	4176.0
49	4424.5
50	4673.0
51	5265.5
52	5858.0
53	6199.0
54	6813.0
55	7086.0
56	7779.5
57	8473.0
58	9849.5
59	11226.0
60	12026.5
61	12827.0
62	12540.0
63	11075.0
64	9897.0
65	8976.5
66	8056.0
67	7010.0
68	5964.0
69	5033.0
70	4102.0
71	3593.0
72	2737.0
73	2390.0
74	2038.5
75	1687.0
76	1403.5
77	1120.0
78	885.5
79	535.0
80	419.0
81	333.5
82	248.0
83	190.5
84	133.0
85	94.5
86	56.0
87	46.0
88	24.0
89	12.0
90	7.5
91	3.0
92	2.5
93	2.0
94	3.0
95	4.0
96	2.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05423198678252973
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	6.30604497471276E-4
24	0.0
25	0.048556546305288245
26	0.0655828677370127
27	0.11161699605241585
28	0.11918425002207117
29	0.15008387039816368
30	0.22954003707954446
31	0.22386459660230298
32	0.29575350931402844
33	0.31908587572046565
34	0.3304367566749486
35	0.3525079140864433
36	0.3846687434574783
37	1.3280530716745071
38	0.45466584267679
39	0.44899040219954844
40	0.6476308189030004
41	0.5133120609416186
42	0.5397974498354122
43	0.6015966905875972
44	0.640063564933345
45	0.6362799379485174
46	0.6823140662639205
47	0.6589816998574834
48	0.8027595252809343
49	0.7592478149554163
50	0.7535723744781748
51	0.728978799076795
52	0.7750129273921982
53	0.788255621839095
54	0.8223082647025438
55	0.8210470557076013
56	0.8235694736974865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	158578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.843408291187934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50093490205103	31.78688090403461
2	10.88369818485171	9.54356846473029
3	5.164974254235825	6.793502251258056
4	3.036274199580013	5.324824376647454
5	1.970485861404367	4.31964080767824
6	1.3836550355262778	3.639849159404205
7	0.9895578632454046	3.036991259821665
8	0.7134021804792451	2.502238645966023
9	0.5091620401001065	2.009105928943485
>10	2.6378620947559184	20.471944405907504
>50	0.1366395305353393	3.9551514081398427
>100	0.06903892069153986	4.902319363341699
>500	0.0028766216954808272	1.0550013242694447
>1k	0.0014383108477404136	0.6589816998574834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1045	0.6589816998574834	No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	961	0.6060109220698962	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	712	0.44899040219954844	No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	344	0.21692794713011893	No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.21629734263264766	No Hit
TAAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.18981195373885407	No Hit
TAAGGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	301	0.18981195373885407	No Hit
TAAGGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	272	0.17152442331218706	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	268	0.16900200532230195	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	246	0.15512870637793388	No Hit
TAAGGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	235	0.14819205690574985	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	234	0.14756145240827856	No Hit
TAAGGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	228	0.1437778254234509	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCC	221	0.139363593941152	No Hit
TAAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	197	0.12422908600184138	No Hit
TAAGGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	186	0.11729243652965733	No Hit
TAAGGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	171	0.10783336906758818	No Hit
TAAGGGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	170	0.1072027645701169	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC	165	0.10404974208276055	No Hit
TAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163	0.10278853308781798	No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.10026611509793289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.30604497471276E-4	0.0	0.0	0.0	0.0
8	0.004414231482298932	0.0	0.0	0.0	0.0
9	0.005044835979770208	0.0	0.0	0.0	0.0
10	0.007567253969655311	0.0	0.0	0.0	0.0
11	0.011981485451954243	0.0	0.0	0.0	0.0
12	0.012612089949425519	0.0	0.0	0.0	0.0
13	0.014503903441839347	0.0	0.0	0.0	0.0
14	0.019548739421609555	0.0	0.0	0.0	0.0
15	0.021440552914023383	0.0	0.0	0.0	0.0
16	0.02207115741149466	0.0	0.0	0.0	0.0
17	0.024593575401379762	0.0	0.0	0.0	0.0
18	0.025854784396322314	0.0	0.0	0.0	0.0
19	0.027115993391264866	0.0	0.0	0.0	0.0
20	0.033422038365977624	0.0	0.0	0.0	0.0
21	0.03657506085333401	0.0	0.0	0.0	0.0
22	0.040989292335632935	0.0	0.0	0.0	0.0
23	0.0472953373103457	0.0	0.0	0.0	0.0
24	0.05297077778758718	0.0	0.0	0.0	0.0
25	0.05801561376735739	0.0	0.0	0.0	0.0
26	0.05927682276229994	0.0	0.0	0.0	0.0
27	0.06116863625471377	0.0	0.0	0.0	0.0
28	0.06873589022436909	0.0	0.0	0.0	0.0
29	0.07062770371678291	0.0	0.0	0.0	0.0
30	0.07315012170666801	0.0	0.0	0.0	0.0
31	0.07567253969655312	0.0	0.0	0.0	0.0
32	0.07693374869149566	0.0	0.0	0.0	0.0
33	0.0788255621839095	0.0	0.0	0.0	0.0
34	0.0813479801737946	0.0	0.0	0.0	0.0
35	0.08765402514850736	0.0	0.0	0.0	0.0
36	0.0920682566308063	0.0	0.0	0.0	0.0
37	0.09396007012322012	0.0	0.0	0.0	0.0
38	0.09648248811310522	0.0	0.0	0.0	0.0
39	0.09837430160551905	0.0	0.0	0.0	0.0
40	0.10404974208276054	0.0	0.0	0.0	0.0
41	0.10909457806253074	0.0	0.0	0.0	0.0
42	0.11413941404230095	0.0	0.0	0.0	0.0
43	0.11666183203218605	0.0	0.0	0.0	0.0
44	0.11918425002207116	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGAAC	15	0.00797271	50.69132	37
CAGGACC	25	1.9168812E-5	50.69132	36
CAGTACG	20	3.899312E-4	50.691315	37
TACCAAG	15	0.007982853	50.675026	35
GTTCGAG	15	0.007982853	50.675026	35
TAAAGGC	20	3.9179192E-4	50.642467	34
CTTTGAC	15	0.008074571	50.528847	33
CGAATAT	15	0.008074571	50.528847	33
GCGAATA	15	0.00808481	50.512657	32
ACATTAC	15	0.00808481	50.512657	32
CGCGATC	15	0.008270772	50.223	39
GTACGTA	20	4.082157E-4	50.223	39
TACGTAG	20	4.082157E-4	50.223	40
TCCTCGA	15	0.008281196	50.207005	38
GTCTACC	15	0.008312525	50.15908	50
TTGCGTA	15	0.008312525	50.15908	48
AGTCTAC	15	0.008312525	50.15908	49
GAACCGC	25	2.0404796E-5	50.15908	48
CGACTGA	15	0.008312525	50.15908	50
CTAGCTT	15	0.008312525	50.15908	50
>>END_MODULE