FastQCFastQC Report
Fri 17 Jun 2016
SRR1526595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295637
Sequences flagged as poor quality0
Sequence length64
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36041.2190625665934913No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT34911.1808400166420308No Hit
AGTTTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG17320.5858535974861062No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.45021428305658634No Hit
AGTTTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13260.4485230197843978No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8460.286161745654299No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG7940.26857260762353835No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT7060.2388063740330202No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.22527626785551197No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.22459976254663658No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.2185112147667579No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA6200.20971664575137752No Hit
AGTTTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.19483352895611847No Hit
AGTTTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.19178925506617914No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5570.18840672852180207No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA5560.1880684758673644No Hit
AGTTTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.18570070728630042No Hit
AGTTTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4260.14409563079046261No Hit
AGTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.14003659893721015No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG3770.12752125072301504No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG3330.11263813392775598No Hit
AGTTTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA3060.10350531225793794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACG150.00464438658.12385655
CGTTCAG150.004656896558.0843846
CAATTGC150.00466002858.07452438
CAATTCA150.00466002858.07452440
GCCGACA150.00466002858.07452436
ACAATTC150.00466002858.07452439
TATCGTT150.00466002858.07452439
CGTTCGG202.000232E-458.0449634
ACCGGGC150.0046725758.0351230
CGCGATT150.0046725758.0351230
TTAGCGA258.622692E-658.03511430
GACGTAT351.6321792E-858.0154328
CGACGTA303.7495556E-758.00559627
ACGACGT303.7495556E-758.00559626
TACCCCG150.00468513757.9957625
AACGACG303.7539394E-757.9957625
TTCGATC150.00469457957.96628620
CGAAATT150.00472615457.86823713
GAATGCT150.00472615457.86823710
TAGGCGA150.00472615457.8682375
Produced by FastQC (version 0.11.5)