FastQCFastQC Report
Fri 17 Jun 2016
SRR1526594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287526
Sequences flagged as poor quality0
Sequence length64
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29051.0103434124218331No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT27800.9668690831437853No Hit
AGTTTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG13820.4806521844980976No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.37109687471741687No Hit
AGTTTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10260.35683729471421716No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG6700.23302240493033674No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6680.23232681566188795No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6630.23058784249076605No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.18676571857849378No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA5260.18293997760202557No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.18154879906512802No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.18085320979667924No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA4970.17285393320951845No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4690.16311568345123573No Hit
AGTTTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.1589421478405431No Hit
AGTTTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.1551164068640749No Hit
AGTTTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4410.15337743369295298No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG3430.11929355953896342No Hit
AGTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.11685899709939275No Hit
AGTTTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3340.11616340783094398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATCG303.579953E-758.39433737
CGAAACG150.00460108458.26106335
AGCCTAC150.00460108458.26106335
TCGATAT150.004633154358.1589656
AGTAAGT150.004633154358.1589655
TGGGTAC150.004636370658.14876644
ATCGTTG303.6947313E-758.12839540
CTATACA201.9997737E-458.0470532
TATACCT303.7623795E-757.97606728
GACGTAT700.057.97606328
CGGTAGT150.004720575657.88505624
CGATCAT202.0346031E-457.84469622
TAGGTAC258.828085E-657.8043917
CGAAAGC407.385097E-1057.80438621
TCGATCA202.0416264E-457.80438621
GGATATC258.873765E-657.7540938
GACTATC150.004763102657.7540939
TAGGCCC351.6912963E-857.754095
CCGAAAG453.274181E-1157.7540920
CGTAGAC150.00476638657.7440419
Produced by FastQC (version 0.11.5)