FastQCFastQC Report
Fri 17 Jun 2016
SRR1526593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256423
Sequences flagged as poor quality0
Sequence length56
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT36871.43785853843064No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG36001.403930224667834No Hit
AGTTTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA14170.552602535653978No Hit
AGTTTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA13420.5233539893067314No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.5030749971726405No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG7900.3080846881909969No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC7370.28741571543894273No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.25231745982224685No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.23632825448575207No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.23398837077797233No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5620.21916910729536743No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG5220.20356988257683595No Hit
AGTTTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.1981101539253499No Hit
AGTTTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.19304040589182717No Hit
AGTTTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.18446083229663485No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4570.17822114240922227No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC4050.15794215027513134No Hit
AGTTTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG3760.146632712354196No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3750.1462427317362327No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA3600.14039302246678342No Hit
AGTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.12089399156861903No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2680.10451480561416096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC150.00801369850.63475837
CCCAGTA150.00803259450.60460335
CTCCGAT150.008038950.59456334
GACGTTT150.008038950.59456334
CGAACAG203.9835877E-450.4843632
ACGTTTC150.00810850850.48435632
TACGTTT150.00811485850.4743631
GCACGGT150.00828126450.21587447
CACTATC150.00828126450.21587447
CAATTAT800.050.2059944
TTATACT204.0977844E-450.19610243
CGTGCAA204.1097446E-450.1664742
TCGATGA301.0209587E-650.1664742
ATTATAC204.1097446E-450.1664742
TTAGCCG204.1901908E-449.9698230
TACGAGG204.1901908E-449.9698230
TGCTACG150.00846329749.9404528
CTTTAGA150.00846329749.9404528
CACGTAT150.00846329749.9404528
GCCTACG204.214567E-449.9111227
Produced by FastQC (version 0.11.5)