##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256423
Sequences flagged as poor quality	0
Sequence length	56
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.93924101972132	39.0	38.0	40.0	35.0	40.0
2	37.185310990043796	39.0	37.0	40.0	33.0	40.0
3	37.595020727469844	39.0	38.0	40.0	33.0	40.0
4	37.18058832476026	39.0	36.0	40.0	33.0	40.0
5	37.54558678433682	39.0	38.0	40.0	33.0	40.0
6	38.050291900492546	39.0	38.0	40.0	35.0	40.0
7	37.74154034544483	39.0	38.0	40.0	35.0	40.0
8	37.85854623025236	39.0	38.0	40.0	35.0	40.0
9	37.92336880857022	39.0	38.0	40.0	35.0	40.0
10	37.5755177967655	39.0	38.0	40.0	33.0	40.0
11	37.971796601708895	39.0	38.0	40.0	35.0	40.0
12	37.84790365918814	39.0	38.0	40.0	34.0	40.0
13	37.81973925895883	39.0	38.0	40.0	34.0	40.0
14	37.79277599903285	39.0	38.0	40.0	34.0	40.0
15	37.77849490880303	39.0	38.0	40.0	33.0	40.0
16	37.73583882881021	39.0	38.0	40.0	34.0	40.0
17	37.65533902965023	39.0	38.0	40.0	33.0	40.0
18	37.59250535248398	39.0	38.0	40.0	33.0	40.0
19	37.46877620182277	39.0	38.0	40.0	33.0	40.0
20	37.44708937965783	39.0	38.0	40.0	33.0	40.0
21	37.42042640480768	39.0	38.0	40.0	33.0	40.0
22	37.273832690515285	39.0	37.0	40.0	33.0	40.0
23	37.10938176372634	39.0	37.0	40.0	33.0	40.0
24	37.008770664097995	39.0	37.0	40.0	33.0	40.0
25	37.01563042316797	39.0	37.0	40.0	33.0	40.0
26	36.72087527249896	39.0	36.0	40.0	32.0	40.0
27	36.63334022299092	39.0	36.0	40.0	32.0	40.0
28	36.418897680785264	39.0	36.0	40.0	31.0	40.0
29	36.41228750931079	39.0	36.0	40.0	31.0	40.0
30	36.16218123959239	38.0	36.0	40.0	30.0	40.0
31	35.86369007460329	38.0	35.0	40.0	30.0	40.0
32	35.581028222897324	38.0	35.0	40.0	29.0	40.0
33	35.5466046337497	38.0	35.0	40.0	29.0	40.0
34	35.82835393080964	39.0	36.0	40.0	29.0	40.0
35	35.643939896187156	38.0	35.0	40.0	29.0	40.0
36	35.266657047144754	39.0	35.0	40.0	28.0	40.0
37	34.68680266590751	39.0	35.0	40.0	23.0	40.0
38	34.84382446192424	38.0	35.0	40.0	26.0	40.0
39	34.77957515511479	38.0	35.0	40.0	25.0	40.0
40	34.388908171263886	38.0	35.0	40.0	23.0	40.0
41	34.07570693736521	38.0	34.0	40.0	21.0	40.0
42	33.978574464849096	38.0	34.0	40.0	20.0	40.0
43	33.81782835393081	38.0	34.0	40.0	19.0	40.0
44	33.37329724712682	38.0	33.0	40.0	13.0	40.0
45	33.36677677119447	38.0	33.0	40.0	14.0	40.0
46	33.01366492085344	38.0	33.0	40.0	4.0	40.0
47	32.78122867293496	38.0	33.0	40.0	4.0	40.0
48	32.39473448169626	38.0	32.0	40.0	4.0	40.0
49	32.34592450755198	38.0	32.0	39.0	4.0	40.0
50	32.01456967588711	38.0	32.0	39.0	4.0	40.0
51	31.468160032446388	38.0	31.0	39.0	4.0	40.0
52	31.289342219691683	38.0	31.0	39.0	4.0	40.0
53	30.92956170078347	37.0	30.0	39.0	4.0	40.0
54	30.4601537303596	37.0	30.0	39.0	4.0	40.0
55	29.950983336128193	36.0	29.0	39.0	4.0	40.0
56	18.471888247154116	21.0	15.0	25.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.7705032497322364
1	2	-1.7601240996662142
1	3	-0.628568758431328
1	4	-4.162268965331428
1	5	-3.0964426340499784
1	6	-1.4580843801814751
1	7	-2.0986992239723747
1	8	-3.2226798315165084
1	9	-3.8191714070268503
1	10	-0.4383391464427646
1	11	-0.8894683799940211
1	12	-1.1178547869338757
1	13	-1.4802488369687694
1	14	-1.189210998901494
1	15	-1.0089285557264702
1	16	-1.2067613070583008
1	17	-1.1047283675426556
1	18	-1.6719875112940983
1	19	-1.538193836601181
1	20	-1.4624044221967765
1	21	-1.274648230891529
1	22	-1.4114069432646303
1	23	-1.9692275657844363
1	24	-2.263942270971114
1	25	-2.17209113965378
1	26	-1.6365141373913232
1	27	-2.0241117206339894
1	28	-1.6018149352588011
1	29	-2.0458766761390805
1	30	-2.1758205376353033
1	31	-2.1795292544333833
1	32	-2.1316935751671338
1	33	-1.8507831824489287
1	34	-2.0603158343173718
1	35	-1.3128843268159187
1	36	-1.1648326567169818
1	37	-0.7019473607055033
1	38	-0.9511298510217472
1	39	-1.0797705961607207
1	40	-0.7421366147091462
1	41	-0.8913265899370799
1	42	-0.9925404187810543
1	43	-0.8885815680230351
1	44	-0.10470142261412008
1	45	-0.5392015087523632
1	46	-0.2754981014443345
1	47	-0.586086853272576
1	48	0.12307282334685254
1	49	-0.12872808682273984
1	50	-0.41864100688279393
1	51	-0.2885366167906085
1	52	-0.011787632660361425
1	53	0.03689567843183639
1	54	0.2495360732708498
1	55	0.06915204826861299
1	56	0.5693503937708257
2	1	0.17353642652501122
2	2	0.6012755546204716
2	3	0.15017471905692048
2	4	0.5228249749926377
2	5	0.8733316830046718
2	6	0.15247118434898255
2	7	0.7167964869208276
2	8	0.27975147007796863
2	9	0.2773859991539851
2	10	0.32134163415852157
2	11	-0.2614332917078883
2	12	-0.3378350780438737
2	13	-0.08009375062120938
2	14	0.05674625542396683
2	15	-0.10151187425121577
2	16	-0.09721541926973032
2	17	0.031844547272939394
2	18	0.02977821139212722
2	19	-0.40593426082696027
2	20	-0.2828596652461641
2	21	-0.10030532851515517
2	22	-0.32609329728152403
2	23	-0.20671969030584592
2	24	-0.3291593110835507
2	25	-0.3023475167720804
2	26	-0.2993467249101016
2	27	-0.0822206364834912
2	28	-0.31603603023303606
2	29	-0.1878776131190989
2	30	0.336739841033868
2	31	-0.16138738273502184
2	32	0.05704327884168947
2	33	0.07823250388893399
2	34	0.34260172759600493
2	35	0.3556347601132117
2	36	0.46386477951183736
2	37	0.5387595745620999
2	38	0.718213132128767
2	39	0.5680541562665411
2	40	1.2251983233370893
2	41	0.6147557027560921
2	42	0.2526575586344961
2	43	0.4908145645111759
2	44	0.9113241666332286
2	45	0.7478859373308993
2	46	0.6023390902003882
2	47	0.721791856279772
2	48	1.0836873529990356
2	49	0.8474112324128171
2	50	1.174499653203469
2	51	1.6219979464636225
2	52	1.524504188150285
2	53	1.8570762894074235
2	54	1.9809943695826995
2	55	1.6441508366565216
2	56	0.6481859537779329
3	1	-0.2959799560348486
3	2	0.26051525733843306
3	3	-0.13178538899013859
3	4	0.22827463696403782
3	5	0.46015848722278463
3	6	-0.0806388313697326
3	7	0.10228643762816603
3	8	-0.04959368535413944
3	9	0.032235145307481616
3	10	-0.08960347243876043
3	11	-0.3832914570443151
3	12	-0.47667860819175445
3	13	-0.40693612094184317
3	14	-0.26789854748170683
3	15	-0.4100564436653471
3	16	-0.6511855384572414
3	17	-0.5017217689020725
3	18	-0.43392377404898497
3	19	-0.4833374686649492
3	20	-0.47287294221595744
3	21	-0.7884491470937505
3	22	-0.8139074807478863
3	23	-0.6876359538308066
3	24	-0.9387400945773763
3	25	-0.43974126286148163
3	26	-0.510709979751617
3	27	-0.8202980702130986
3	28	-0.6381322653727466
3	29	-0.6417058060777237
3	30	-0.877294068652219
3	31	-0.44281335981317227
3	32	-0.7905157426303759
3	33	-1.0075794514882404
3	34	-0.5119009964719368
3	35	-0.5357993052338941
3	36	-0.7542285255379895
3	37	-0.01167001934575751
3	38	-0.22602359471665778
3	39	-0.21751515104189423
3	40	0.019435813226756693
3	41	-0.4025806624063648
3	42	-0.13461791559750225
3	43	-0.02184433945208042
3	44	-0.28135976518113637
3	45	0.005260759923110925
3	46	0.030635822844942595
3	47	0.25746417456078774
3	48	0.6142167530936504
3	49	0.6055567296887503
3	50	0.26806413807230456
3	51	0.9414235267587685
3	52	1.3365511306320137
3	53	0.6602108751507068
3	54	1.2464269393630971
3	55	0.6159792432675353
3	56	0.005290658708727136
4	1	-0.2363659127890898
4	2	0.07784456060124256
4	3	-0.3173701367232269
4	4	-0.14912596754653862
4	5	0.44056614435677943
4	6	-0.12982119206104414
4	7	0.12471533013378888
4	8	0.15341336726928034
4	9	0.15183523830910417
4	10	0.12403676626541937
4	11	-0.8779826141395617
4	12	-0.6087564195534512
4	13	-0.5002012530816558
4	14	-0.18151553577900614
4	15	-0.48337272670030274
4	16	-0.33029892294147345
4	17	-0.7459130422493558
4	18	-0.6037292456447432
4	19	-0.4754323303250274
4	20	-0.42945668243141455
4	21	-0.39729980198021053
4	22	-0.1899285611167869
4	23	-0.5574956763759502
4	24	-0.21732646983894455
4	25	-0.4245974978455891
4	26	-0.12411771438697627
4	27	0.08710330931103982
4	28	-0.47156635046923867
4	29	-0.5298868492459832
4	30	-0.5538499083794122
4	31	-0.43772927084161495
4	32	-0.2199042569293752
4	33	-0.1876771031971458
4	34	-0.27096478008814984
4	35	-1.206542543240559
4	36	0.28170110323051034
4	37	0.3054035294413495
4	38	0.49235197685584353
4	39	0.44583445757625384
4	40	0.7740665192641103
4	41	0.671262629851249
4	42	0.35336162404444593
4	43	0.5961669757459376
4	44	0.7302809385311804
4	45	0.8030907219474486
4	46	0.1170033342763972
4	47	0.0185894942538809
4	48	0.3993054921465884
4	49	0.21423688159140397
4	50	-0.4551165175891718
4	51	0.0019055851947875624
4	52	0.332211054680684
4	53	0.1582345905657263
4	54	0.18270532423483488
4	55	0.5889157696567473
4	56	0.3588983468432687
5	1	0.007191510180092564
5	2	0.05730301064792798
5	3	0.19028625916845954
5	4	-0.19585799369033197
5	5	0.485944295617287
5	6	0.14861018048797803
5	7	0.3191130892374261
5	8	0.15126584159234113
5	9	0.2812470030149896
5	10	0.050320613137500914
5	11	-0.03974732002191672
5	12	0.09609885589005529
5	13	0.0350928645654065
5	14	-0.24612804745034111
5	15	-0.043596816336155086
5	16	-0.4127452347995515
5	17	-0.25912499369660225
5	18	-0.1317404501265358
5	19	-0.404003758896458
5	20	-0.6357125180990124
5	21	-0.13516161337143728
5	22	-0.030404751592982393
5	23	-0.3050680386541984
5	24	-0.17675690868114913
5	25	0.11013639571183376
5	26	-0.10908503464840891
5	27	-0.02344757771043504
5	28	0.11092664672963792
5	29	-0.01574119098267346
5	30	-0.1778461735521475
5	31	-0.274750746098384
5	32	0.17673439853280826
5	33	-0.1366967110402797
5	34	-0.5782476932534166
5	35	0.02777118224963715
5	36	-0.7485976829506242
5	37	-2.0887608029582765
5	38	-0.6886937747781445
5	39	-0.6047257665133827
5	40	-0.6064190082802412
5	41	-0.2375608995605134
5	42	-0.0719885160115794
5	43	-0.0666714929748835
5	44	0.07981408512314658
5	45	-0.10872066927571211
5	46	0.04646178432307835
5	47	0.3445932290811413
5	48	0.39953891885060244
5	49	-0.18851755351597177
5	50	0.03475276345658074
5	51	-0.01774894328326937
5	52	-0.06601490236880281
5	53	0.08283776516890029
5	54	0.1655718041601375
5	55	0.4431641356698215
5	56	0.2490010545930339
6	1	-0.0455831429882565
6	2	-0.21897495460641636
6	3	-0.3292737958246619
6	4	-0.09896385545761177
6	5	0.2259142993668135
6	6	0.0029408891493929445
6	7	0.14307259429929786
6	8	-0.13863542074986412
6	9	-0.20300496548894387
6	10	0.11015688360369325
6	11	-0.32649272684606245
6	12	-0.03707199775323744
6	13	-0.12107511443721819
6	14	-0.24619053963881754
6	15	-0.2303234755037593
6	16	-0.44286646964519605
6	17	-0.25815636477521764
6	18	-0.38333400093268466
6	19	-0.48724335394707197
6	20	-0.3743076937020646
6	21	-0.23580528291274305
6	22	-0.4258865663661311
6	23	-0.023884436604454606
6	24	-0.5648021530256031
6	25	-0.24513169577673466
6	26	-0.30681031898787126
6	27	-0.47701588167243614
6	28	-0.41278913879716583
6	29	-0.5934502273531308
6	30	-0.06729749213732816
6	31	-0.05443453562469358
6	32	-0.025740292130862485
6	33	0.10477310523268102
6	34	-0.07206096659425043
6	35	0.0074299749005533045
6	36	2.748890289439032E-5
6	37	-1.0532964859978975
6	38	0.17394839495064218
6	39	-0.29004635144026736
6	40	0.23878784086361549
6	41	0.18566744689618275
6	42	0.06702535725425918
6	43	0.09349598609023246
6	44	0.5059795644382348
6	45	0.2167698944038321
6	46	-0.17797891059005622
6	47	-0.15968748582949388
6	48	0.2130309823426657
6	49	0.07448082168713199
6	50	-0.2248710335687889
6	51	-1.0597124478452002
6	52	-0.8703518602490696
6	53	-1.0071197401429366
6	54	-0.9095750524827828
6	55	-0.9920939570685867
6	56	-1.2719338285465724
7	1	-0.13873782929763223
7	2	-0.23967599600850065
7	3	-0.02367943463593747
7	4	-0.09191534136058266
7	5	0.25648961051744124
7	6	0.17280372887507411
7	7	0.46600249015909156
7	8	0.007192620906216973
7	9	0.10785990624079744
7	10	0.1253334139567528
7	11	-7.688253982607307E-4
7	12	-0.01014794390513174
7	13	-0.14904692038083311
7	14	-0.181483910225559
7	15	-0.19336640158961416
7	16	-0.23814206019637396
7	17	-0.200625249712985
7	18	-0.23331495089458087
7	19	-0.01978716903470712
7	20	0.03850663192658743
7	21	-0.02981087092391732
7	22	-0.14772426004152095
7	23	-0.1028150944657682
7	24	0.061658349895402864
7	25	-0.23874993301320302
7	26	-0.2766477586627474
7	27	-0.00565443894955564
7	28	-0.09452650227189707
7	29	-0.42107913261094154
7	30	-0.20671202096638552
7	31	0.0642789518022795
7	32	-0.26335776736581096
7	33	0.37674414917476895
7	34	-0.030436633345580333
7	35	-0.4507234414062822
7	36	0.11308946502684591
7	37	0.37488487036481644
7	38	-0.10894467083549131
7	39	-0.1323755360986354
7	40	0.1259798652476647
7	41	0.5890520036652944
7	42	0.5505153242341976
7	43	0.44395318700463804
7	44	0.6463783452357958
7	45	0.5874518857678126
7	46	0.47797740132256905
7	47	0.5590069515593825
7	48	0.44690195008460165
7	49	0.7888890005034881
7	50	0.4294660251053273
7	51	1.1569182354161711
7	52	1.3154495113536733
7	53	1.0735571712108865
7	54	1.3759532685738591
7	55	1.5161928095049468
7	56	0.6386579926370679
8	1	0.32521872106445215
8	2	0.923062647836133
8	3	0.367383764837399
8	4	0.7219424598244117
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114	3	0.15104116480525676
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115	16	0.10375517300520443
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115	38	1.0743716622003348
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115	44	1.0822765984552234
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115	51	0.43154210383386626
115	52	0.6132309725292444
115	53	0.707641705190234
115	54	0.34919373778206975
115	55	0.4064930772596327
115	56	0.2661602239261178
116	1	0.2575664633109511
116	2	1.151103785551065
116	3	0.5053718734666788
116	4	0.3341070106841215
116	5	0.8518985513353172
116	6	-0.27658666990572556
116	7	-2.305746303338502
116	8	-0.14257242862387898
116	9	-0.10064276076453638
116	10	-0.5788194943490623
116	11	-0.4839730418066921
116	12	-0.8244735725563856
116	13	-0.5659570042010031
116	14	-0.2737495947569286
116	15	-0.26969355424391495
116	16	0.2972910106697668
116	17	0.15129245412241943
116	18	0.14028804631141156
116	19	0.0836227877852096
116	20	0.342227739368802
116	21	0.33112385094552366
116	22	-0.08730388920078269
116	23	0.03910768937979725
116	24	0.0021269808326636053
116	25	-0.020722246957831203
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116	27	0.49936207108346764
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116	30	0.428765499988657
116	31	0.3432032596508918
116	32	0.49000773936520403
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116	34	0.3058917893112607
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116	37	0.7695381596713915
116	38	0.49678304061993117
116	39	0.05641034147311785
116	40	0.37658311645417086
116	41	0.4848800453213755
116	42	0.29143480607316263
116	43	0.18798417506661025
116	44	0.5453496431783904
116	45	0.13802973692351372
116	46	-8.13556259345205E-4
116	47	-0.3683489031523237
116	48	0.37838531305132506
116	49	-0.2404798082341273
116	50	-0.5673907186081593
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116	53	0.15429758269171145
116	54	-0.3702049737426236
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116	56	-0.004217293113502052
117	1	-0.0998587029012441
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117	3	-0.1148523981102727
117	4	-0.04205341089742376
117	5	0.4835355500178764
117	6	-0.41948320472602774
117	7	-2.4563177056486154
117	8	-0.7051049817045509
117	9	-0.5164183666348166
117	10	-0.05086214271451439
117	11	-0.8824840255990836
117	12	-0.8010353544737256
117	13	-0.6464894960052803
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117	15	-0.9018514022172042
117	16	-0.27510704074111914
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117	30	-0.8268795871770038
117	31	-0.7829188641886802
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117	35	-0.7734609837685227
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118	1	-0.3211153681267831
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118	4	-0.0051172529495886465
118	5	0.1954681051410958
118	6	0.022684254562051365
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118	8	0.23010182042832383
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118	15	-0.009734382473723713
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118	20	-0.13359611598261267
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118	30	-0.0677932634992402
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118	33	0.17071069636712366
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118	35	0.3333267378051872
118	36	0.7601324757795282
118	37	1.0966889324914604
118	38	0.7964914104070431
118	39	0.5743747625871478
118	40	1.334850832989602
118	41	1.0124391004394866
118	42	1.0938844998614385
118	43	1.0338576075542179
118	44	1.3880151433242034
118	45	0.5100623995073548
118	46	0.790641678502972
118	47	1.2564979909859062
118	48	1.3315495008611862
118	49	1.1900538750554546
118	50	1.0403083190121336
118	51	1.1084415329072073
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118	53	0.8323086646398004
118	54	0.9478469364352691
118	55	0.3320530245587108
118	56	-0.06528465969267927
119	1	0.20330679840064647
119	2	1.0263924007899448
119	3	0.24375326000388498
119	4	0.38413365209078876
119	5	0.49596935827392485
119	6	0.05838461657820204
119	7	0.28151910427502
119	8	0.16463259180120104
119	9	0.17974324361649252
119	10	0.025466811967909564
119	11	-0.3549102272900839
119	12	-0.41058451921436756
119	13	-0.4185412206476258
119	14	-0.6187262095221868
119	15	-0.18269456069705825
119	16	-0.5915255188429924
119	17	-0.48949257932734724
119	18	-0.4750988461165093
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119	20	-1.1591043309645102
119	21	-0.7853286977490512
119	22	-1.0573471074827054
119	23	-0.5158032099156813
119	24	-0.4434653464422169
119	25	-0.8660540804786407
119	26	-0.6149329878647833
119	27	-0.498802632012854
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119	29	-0.29080384762428224
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119	32	-0.7217618420686946
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119	37	-0.36298803771182975
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119	40	-1.0019912722735569
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119	44	-0.24829786140985277
119	45	-0.36540404438013496
119	46	-0.6003636125023206
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119	48	-0.12155548382274617
119	49	-1.0584005944516441
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119	51	-0.23433206776113025
119	52	0.02003355210070268
119	53	0.007858650716102034
119	54	0.35876311577250064
119	55	-0.2895008601530691
119	56	0.07523330187791188
120	1	-0.32018944220085643
120	2	-0.35257922181494905
120	3	-0.007550402377873411
120	4	-0.2894959865218709
120	5	-0.38060716367611036
120	6	-0.24251885177008603
120	7	0.07890571596553997
120	8	-0.29925899199700723
120	9	-0.7268175131140424
120	10	-0.04805368281743938
120	11	-0.21447850281761305
120	12	-0.49805116971158014
120	13	-0.6369501461872815
120	14	-0.3589032650642636
120	15	-0.5844954338476782
120	16	-0.33088399568024585
120	17	-0.34175428197104907
120	18	-0.390573015410709
120	19	-0.4552710400024438
120	20	-0.5179449809766368
120	21	-0.46932699995618066
120	22	-0.47836942133184124
120	23	-0.6673312234980244
120	24	-0.26495455333040496
120	25	-0.42826606204546636
120	26	-0.3653574360821068
120	27	-0.25565443894955564
120	28	-0.4574297280783455
120	29	-0.3122081648690056
120	30	-0.6784862145147699
120	31	-0.5002371772299767
120	32	-0.4488416383335547
120	33	-0.7361590766316795
120	34	-0.7481785688294522
120	35	-0.8700782801159548
120	36	-0.43529763174735336
120	37	-0.39930867802227965
120	38	-0.661364025674203
120	39	-0.42269811674379554
120	40	-0.6482136831394314
120	41	-0.48352864149599384
120	42	-0.6067427402819305
120	43	-0.48749842589859327
120	44	-0.555234557990012
120	45	-0.2512577916515397
120	46	-0.35266775996775834
120	47	0.1396521128497028
120	48	-0.21035611443152646
120	49	0.0711470650196162
120	50	-0.001985587797900479
120	51	0.18917629993229568
120	52	0.5977075758698049
120	53	0.29936362282379037
120	54	0.4928887524448271
120	55	0.7540960353113988
120	56	-0.05489039445970434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	218.0
5	36.0
6	59.0
7	69.0
8	160.0
9	196.0
10	196.0
11	348.0
12	346.0
13	436.0
14	565.0
15	652.0
16	788.0
17	877.0
18	1059.0
19	1193.0
20	1301.0
21	1564.0
22	1766.0
23	1813.0
24	2392.0
25	2959.0
26	2951.0
27	3330.0
28	3902.0
29	4106.0
30	5005.0
31	6408.0
32	8232.0
33	9861.0
34	13208.0
35	17137.0
36	23358.0
37	37994.0
38	49735.0
39	52203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.49313251131575	0.25909161854221946	0.1740284064304667	0.07374746371156547
8	99.43413812333527	0.4445779044781474	0.06317686011005252	0.0581071120765298
9	97.61994828857006	1.3505028800068637	0.38257098622198477	0.6469778452010936
10	36.27716702479887	40.66444897688585	9.848960506662818	13.209423491652464
11	25.14946007183443	38.346794164330035	19.575467099285166	16.928278664550373
12	41.607032130503114	19.34342863159701	19.584826634116283	19.464712603783592
13	23.59733721234055	19.021694621777296	27.332961551810875	30.048006614071284
14	24.655744609492906	27.552910620342168	25.99922783837643	21.79211693178849
15	33.42601872686927	21.780807493867556	25.144780304418866	19.6483934748443
16	26.967549712779277	24.10080219013115	26.267924484153138	22.663723612936437
17	30.44890668933754	23.365298744652392	22.072902976722055	24.11289158928801
18	27.97564961021437	24.927171119595357	25.458324721261356	21.63885454892892
19	23.867983761207068	25.37213900469147	26.694953260822935	24.06492397327853
20	21.513280789944737	31.359901412899777	22.607956384567686	24.518861412587796
21	22.560768729794127	22.92267074326406	30.60450895590489	23.91205157103692
22	24.260304262878137	23.224515741567643	25.087063172960306	27.428116822593918
23	26.117001985001348	27.076744285808996	25.770699196249947	21.035554532939713
24	20.951010443721678	30.686134575036462	28.09977303039521	20.26308195084665
25	25.226592585945212	23.24568762022185	26.983538629012433	24.5441811648205
26	24.545287812035387	22.234362622482546	29.48556354654044	23.734786018941627
27	20.21208063124441	22.23176602415189	30.557648684842402	26.99850465976129
28	20.347870222152814	28.02912583453715	31.88498028344981	19.738023659860225
29	24.854705661792277	22.29408823897013	33.37551556055493	19.475690538682663
30	19.026306531681193	25.709260055505613	37.95371926670054	17.31071414611265
31	20.546510036942202	24.806004573796447	37.650163210257816	16.99732217900354
32	19.255640009545083	22.157110499982398	42.01401249467001	16.57323699580251
33	20.212874192917237	22.599491293288985	38.96732537663862	18.220309137155155
34	17.107251050905305	25.638175456175095	41.7724095282081	15.482163964711502
35	15.54500291654759	27.749090780969233	43.56695727747134	13.138949025011842
36	17.24298626231614	26.354971099171355	41.1660583655759	15.235984272936607
37	15.608504311861674	24.088696635522147	45.11579355257071	15.187005500045473
38	19.028416238522404	23.334156310866046	44.10571734248798	13.531710108123571
39	16.73511615512431	22.83051697651817	42.678386682133116	17.75598018622441
40	16.238353076986506	23.97312716692647	45.253081305633316	14.535438450453706
41	14.828524597877829	25.873061931911757	44.617719255593634	14.680694214616777
42	17.11697929660226	22.890630516133587	43.677187036644774	16.315203150619375
43	16.417558953747605	23.259255188871794	45.89639526486011	14.426790592520488
44	16.296755803864137	22.875842654720632	44.91266229814567	15.91473924326956
45	14.801930242693858	25.316177365587812	44.1509641397345	15.730928251983839
46	15.56564498138344	24.85075330306506	42.125272964353606	17.4583287511979
47	16.00557617168326	26.566138501109343	42.96832970096798	14.45995562623942
48	16.828809840268658	27.49762093888369	41.01933921085953	14.654230009988126
49	16.25792554216015	28.09680855663741	40.95220500080582	14.69306090039662
50	15.821744974884247	27.690608516559102	41.87727476397482	14.610371744581837
51	14.162206345837951	30.269269764919475	41.42360865498731	14.144915234255265
52	16.789400224105055	27.318799268709824	41.5195895339008	14.37221097328432
53	16.84920032561121	26.517257787338128	41.25745700791612	15.376084879134536
54	15.742190265660769	29.484648001447482	39.525004523391836	15.24815720949991
55	16.78624813153961	29.50475965699001	38.57446306348832	15.134529147982063
56	17.634031304457288	27.870722583030766	38.231325699315136	16.263920413196807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	6.5
2	13.0
3	12.5
4	21.5
5	31.0
6	225.0
7	419.0
8	387.0
9	355.0
10	443.0
11	531.0
12	710.5
13	1967.5
14	3045.0
15	3837.5
16	4630.0
17	5160.5
18	5691.0
19	6826.0
20	7961.0
21	9249.5
22	11472.5
23	12407.0
24	13246.0
25	14085.0
26	14290.5
27	14496.0
28	14113.5
29	11428.5
30	9126.0
31	9673.5
32	10221.0
33	8024.0
34	5827.0
35	5585.0
36	5343.0
37	5160.0
38	5186.5
39	5396.0
40	5935.0
41	6474.0
42	8513.5
43	10553.0
44	8789.0
45	7025.0
46	7162.5
47	7819.5
48	8339.0
49	9632.5
50	10926.0
51	10787.0
52	10648.0
53	10186.5
54	9656.0
55	9587.0
56	9812.5
57	10038.0
58	9316.0
59	8594.0
60	7657.5
61	6721.0
62	5955.5
63	4647.5
64	4105.0
65	4365.5
66	4626.0
67	3503.5
68	2381.0
69	2015.5
70	1650.0
71	1425.5
72	986.0
73	771.0
74	580.5
75	390.0
76	308.5
77	227.0
78	180.5
79	94.5
80	55.0
81	39.0
82	23.0
83	15.5
84	8.0
85	6.0
86	4.0
87	3.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.055767228368750074
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	3.8998061796328726E-4
25	0.04913755786337419
26	0.06317686011005252
27	0.11465430168120644
28	0.11426432106324315
29	0.1524824216236453
30	0.2312585064522293
31	0.24100802190131151
32	0.30964461066285004
33	0.34045307948194975
34	0.3622919940878938
35	0.3837409280758747
36	0.41610931936682743
37	1.3711718527589178
38	0.48825573369003555
39	0.48630583060021915
40	0.6793462364920464
41	0.5463628457665655
42	0.5806811401473347
43	0.642298077785534
44	0.6715466241327805
45	0.6785662752561198
46	0.7062548991315132
47	0.6894857325590917
48	0.8271488907001322
49	0.7893207707576934
50	0.7803512165445378
51	0.7631920693541531
52	0.8103797241277109
53	0.8314386774977284
54	0.8536675727216357
55	0.8591273013731218
56	0.8618571656988647
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	256423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.515129962346876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7643544843907	28.547611131963702
2	13.321440163058524	11.060826393100038
3	5.983854985672602	7.452615522181061
4	3.3386531465162252	5.544184771072777
5	2.0982816132060176	4.355521693492534
6	1.3698326128541143	3.412126736957985
7	1.0560004218759897	3.068799632813239
8	0.7421871551068424	2.464959696051605
9	0.562866544984065	2.103072998982356
>10	2.5911783620659796	18.012808539417385
>50	0.10559973307273327	3.0687906497525628
>100	0.05279986653636663	4.16396014308744
>500	0.007969791175300625	2.0563378855215286
>1k	0.004981119484562891	4.688384205605802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3687	1.43785853843064	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	3600	1.403930224667834	No Hit
AGTTTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1417	0.552602535653978	No Hit
AGTTTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	1342	0.5233539893067314	No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.5030749971726405	No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	790	0.3080846881909969	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	737	0.28741571543894273	No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.25231745982224685	No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.23632825448575207	No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	600	0.23398837077797233	No Hit
AGTTTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	562	0.21916910729536743	No Hit
AGTTTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	522	0.20356988257683595	No Hit
AGTTTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	508	0.1981101539253499	No Hit
AGTTTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	495	0.19304040589182717	No Hit
AGTTTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	473	0.18446083229663485	No Hit
AGTTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	457	0.17822114240922227	No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	405	0.15794215027513134	No Hit
AGTTTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	376	0.146632712354196	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	375	0.1462427317362327	No Hit
AGTTTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	360	0.14039302246678342	No Hit
AGTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	310	0.12089399156861903	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	268	0.10451480561416096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.016769166572421352	0.0	0.0	0.0	0.0
7	0.016769166572421352	0.0	0.0	0.0	0.0
8	0.01793910842631121	0.0	0.0	0.0	0.0
9	0.021448933987980798	0.0	0.0	0.0	0.0
10	0.0487475772454109	0.0	0.0	0.0	0.0
11	0.07097647246931828	0.0	0.0	0.0	0.0
12	0.07409631741302457	0.0	0.0	0.0	0.0
13	0.076046220502841	0.0	0.0	0.0	0.0
14	0.09593523201896866	0.0	0.0	0.0	0.0
15	0.09905507696267496	0.0	0.0	0.0	0.0
16	0.10217492190638125	0.0	0.0	0.0	0.0
17	0.10763465055786728	0.0	0.0	0.0	0.0
18	0.11270439859139	0.0	0.0	0.0	0.0
19	0.11777414662491274	0.0	0.0	0.0	0.0
20	0.1345433131973341	0.0	0.0	0.0	0.0
21	0.13961306123085682	0.0	0.0	0.0	0.0
22	0.15716218903920476	0.0	0.0	0.0	0.0
23	0.17159147190384638	0.0	0.0	0.0	0.0
24	0.18680071600441459	0.0	0.0	0.0	0.0
25	0.1926504252738639	0.0	0.0	0.0	0.0
26	0.19733019268942334	0.0	0.0	0.0	0.0
27	0.2074696887564688	0.0	0.0	0.0	0.0
28	0.23866813819353178	0.0	0.0	0.0	0.0
29	0.24295792499112795	0.0	0.0	0.0	0.0
30	0.2655768008329986	0.0	0.0	0.0	0.0
31	0.27571629690004407	0.0	0.0	0.0	0.0
32	0.28234596740541995	0.0	0.0	0.0	0.0
33	0.28936561852875914	0.0	0.0	0.0	0.0
34	0.29794519212395143	0.0	0.0	0.0	0.0
35	0.3396731182460232	0.0	0.0	0.0	0.0
36	0.35215249802084836	0.0	0.0	0.0	0.0
37	0.3595621297621508	0.0	0.0	0.0	0.0
38	0.369311645211233	0.0	0.0	0.0	0.0
39	0.3786711800423519	0.0	0.0	0.0	0.0
40	0.3907605791992138	0.0	0.0	0.0	0.0
41	0.40090007526625926	0.0	0.0	0.0	0.0
42	0.41064959071534146	0.0	0.0	0.0	0.0
43	0.42234900925424007	0.0	0.0	0.0	0.0
44	0.4320985247033222	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	15	0.008013698	50.634758	37
CCCAGTA	15	0.008032594	50.604603	35
CTCCGAT	15	0.0080389	50.594563	34
GACGTTT	15	0.0080389	50.594563	34
CGAACAG	20	3.9835877E-4	50.48436	32
ACGTTTC	15	0.008108508	50.484356	32
TACGTTT	15	0.008114858	50.47436	31
GCACGGT	15	0.008281264	50.215874	47
CACTATC	15	0.008281264	50.215874	47
CAATTAT	80	0.0	50.20599	44
TTATACT	20	4.0977844E-4	50.196102	43
CGTGCAA	20	4.1097446E-4	50.16647	42
TCGATGA	30	1.0209587E-6	50.16647	42
ATTATAC	20	4.1097446E-4	50.16647	42
TTAGCCG	20	4.1901908E-4	49.96982	30
TACGAGG	20	4.1901908E-4	49.96982	30
TGCTACG	15	0.008463297	49.94045	28
CTTTAGA	15	0.008463297	49.94045	28
CACGTAT	15	0.008463297	49.94045	28
GCCTACG	20	4.214567E-4	49.91112	27
>>END_MODULE