FastQCFastQC Report
Fri 17 Jun 2016
SRR1526592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148426
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5930.39952568956921297No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4610.3105924837966394No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.30116017409348766No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA3430.2310915877272176No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3340.22502796006090578No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA3260.2196380688019619No Hit
ATTTGCGGGGGAAATTTTGGTTTCTGTTCTTTAGGAGGATACAGAATGTTGAAGAAGATATCTC3210.21626938676512203No Hit
ATTTGCGGGAGATGGAGTTGGCTAGATGTTGCAATGAGTCTGATACAGAGCTCTAGATACTGAG2970.2000997129882905No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2840.1913411396925067No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2570.1731502566935712No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2430.16371794699041947No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2420.1630442105830515No Hit
ATTTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.15495937369463572No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT2230.15024321884305983No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC2070.13946343632517214No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1990.1340735450662283No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1333998086588603No Hit
ATTTGCGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT1950.13137859943675637No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1920.12935739021465242No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1850.12464123536307654No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1800.12127255332623664No Hit
ATTTGCGGGACATGTAATGTGTGCAAACTTTGCTTTATACTTGGCTAAGAAGCCATCAGCTTTG1770.1192513441041327No Hit
ATTTGCGGGAGAGTTGTGAAATTGTCTTTATAAAATTCCAGAGCATTTCATCGCTGTAAAAAGT1660.11184024362308491No Hit
ATTTGCGGGAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCTATGCCATGAACTCTACCGCCA1530.10308167032730114No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1500.1010604611051972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG201.9737886E-458.18212554
ACGCTAA201.9737886E-458.18212555
ATACGTT150.00462144658.18212553
GATAGTC150.00462144658.18212556
TCTCGTC150.004640063758.12309650
GGTCGCA150.004652506758.08381349
AATCACC258.576086E-658.0641942
CTACGTA150.00465873758.0641939
ACGGACT150.00465873758.0641942
CGCCACA150.00465873758.0641942
GTCTCGC150.004664974358.0445836
CGACATC150.004664974358.0445838
CCGACAT150.004664974358.0445837
TTAGGAG202.0037117E-458.005431
CTCACGT202.0037117E-458.005433
TTCACGA150.00467746758.005432
CACGATA150.00467746758.005434
GTAGGAT150.00467746758.005430
ATGAGTC150.00467746758.005434
TAGGATT150.00467746758.005431
Produced by FastQC (version 0.11.5)