FastQCFastQC Report
Fri 17 Jun 2016
SRR1526591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142802
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5190.36344028795114913No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.2766067702133023No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3760.26330163443089033No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3330.23319001134437892No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2550.17856892760605594No Hit
ATTTGCGGGGGAAATTTTGGTTTCTGTTCTTTAGGAGGATACAGAATGTTGAAGAAGATATCTC2500.17506757608436857No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2380.16666433243231887No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2250.1575608184759317No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2200.15405946695424433No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2060.1442556826935197No Hit
ATTTGCGGGAGATGGAGTTGGCTAGATGTTGCAATGAGTCTGATACAGAGCTCTAGATACTGAG1970.13795324995448244No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1890.13235108751978264No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1880.13165081721544517No Hit
ATTTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.1253483844764079No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1670.11694514082435821No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.11554460021568325No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1620.11344378930267082No Hit
ATTTGCGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT1600.11204324869399589No Hit
ATTTGCGGGAGAGTTGTGAAATTGTCTTTATAAAATTCCAGAGCATTTCATCGCTGTAAAAAGT1490.10434027534628368No Hit
ATTTGCGGGACATGTAATGTGTGCAAACTTTGCTTTATACTTGGCTAAGAAGCCATCAGCTTTG1470.10293973473760872No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT1460.10223946443327124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGT303.6209894E-758.26751735
CCCTATA150.00460717258.2263158
ATATCTC303.6387428E-758.2263158
TCGTGCC150.00460717258.2263156
TCTACCG303.6387428E-758.2263155
TCCACTA150.00460717258.2263157
TCGGTTA150.00460717258.2263138
TACTTGG258.433597E-658.22630738
CACGCGA258.433597E-658.22630758
ACCTCGT201.9661087E-458.22630338
GTCGAGG150.00461362858.2057354
TGGACGA201.96955E-458.20572354
TCACGCT150.004620091558.1851653
TCTAATA150.00462656158.16460848
AATCAAC150.00463952158.12354746
TATCTCC150.00463952158.12354741
CTAGCTT150.00463952158.12354751
CGCCACA150.00463952158.12354742
CACGTCC201.9868283E-458.1030340
CGCGGCA303.701516E-758.08254226
Produced by FastQC (version 0.11.5)