FastQCFastQC Report
Fri 17 Jun 2016
SRR1526590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131796
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4870.36951045555252054No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4740.35964672675953746No Hit
ATTTGCGGGGGAAATTTTGGTTTCTGTTCTTTAGGAGGATACAGAATGTTGAAGAA4550.34523050775440833No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4450.33764302406749824No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3500.26556192904185255No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3480.26404443230447056No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3160.2397644845063583No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2680.20334456280918997No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2620.19879207259704393No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2320.1760296215363137No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2290.1737533764302407No Hit
ATTTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.1737533764302407No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2180.1654071443746396No Hit
ATTTGCGGGAGATGGAGTTGGCTAGATGTTGCAATGAGTCTGATACAGAGCTCTAG2080.1578196606877295No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.1540259188442745No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2000.15174967373820147No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1980.15023217700081945No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1890.14340344168260039No Hit
ATTTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1760.1335397128896173No Hit
ATTTGCGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1740.13202221615223528No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1620.12291723572794319No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC1580.11988224225317914No Hit
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1550.11760599714710612No Hit
ATTTGCGGGGACCTATTGTAGAACACAGGGGCACTGCATTCTGGGAATCAACCTAC1480.11229475856626908No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC1460.11077726182888707No Hit
ATTTGCGGGAGAGTTGTGAAATTGTCTTTATAAAATTCCAGAGCATTTCATCGCTG1400.10622477161674101No Hit
ATTTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1360.103189778141977No Hit
ATTTGCGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1320.10015478466721298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAG150.00800472850.63513637
ATGTCCG150.00802920350.5960634
AGCGATG203.9783854E-450.47921833
TCAGGGT150.00814002850.4209931
ACGGCTA150.00814002850.4209931
GCAGTAT150.00825198850.24712840
CCCGTCA204.0699504E-450.24712840
GCCCTAG150.00827702350.20865248
AAATTCG150.00827702350.20865248
AAGATTA150.00827702350.20865248
ATTCGTA150.00828956250.18943850
CCGTTCG252.0312877E-550.18943846
TTCCTAA150.00828956250.18943850
CACTACG204.1008415E-450.1702444
ACAATTA301.0165895E-650.1702443
CACAATT301.0165895E-650.1702442
TCGTATC301.0219719E-650.1318841
CGCGATT150.0084411349.9630
TTACCTA150.0084411349.9630
AGTCGCC150.0084411349.9630
Produced by FastQC (version 0.11.5)