FastQCFastQC Report
Fri 17 Jun 2016
SRR1526589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199399
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44592.2362198406210663No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36761.8435398372108187No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17140.8595830470564045No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10240.5135431973079103No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6970.34955039894884127No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.3435323146053892No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC6120.30692230151605576Illumina Single End Adapter 2 (96% over 31bp)
GGACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.245236936995672No Hit
GGACATGGGGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.24072337373808295No Hit
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.21113445904944356No Hit
GGACATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4050.2031103465915075No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.20010130441978144No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.19909829036253943No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA3790.1900711638473613Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.18305006544666724No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.17602896704597315No Hit
GGACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.17352143190286812No Hit
GGACATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3100.1554671788725119No Hit
GGACATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.14844608047181782No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.14593854532871278No Hit
GGACATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.12086319389766247No Hit
GGACATGGGGTGCTGGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.11233757441110537No Hit
GGACATGGGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.11033154629662134No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA2180.10932853223937933Illumina Single End Adapter 2 (100% over 34bp)
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC2110.1058179830390323No Hit
GGACATGGGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.10280894086730624No Hit
GGACATGGGGAGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.10280894086730624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT258.511204E-658.15092552
ACCAAGA150.00466606358.04854238
ATCGGGA150.00466606358.04854236
ACCTTTG201.9980321E-458.0485437
CTACCTT202.0005183E-458.03394335
GTCGAGT202.007992E-457.990231
AGACGTA202.0129868E-457.9610827
ACGCAAC150.00470335457.93197624
CGATCCC150.00470803157.9174420
TCGATCC150.004712711657.9029119
TAGATCC150.004712711657.9029119
TGCACTT150.004712711657.9029119
GGACTAC258.742674E-657.888398
TGGAAGC850.057.888396
ATTCGTG258.742674E-657.8883914
GGCCCAT258.742674E-657.888397
TTGGCCA150.00471739557.88838611
GATGTAC202.0255169E-457.8883869
GGGTCCA202.0255169E-457.8883867
GGTCCAT150.00471739557.8883867
Produced by FastQC (version 0.11.5)