FastQCFastQC Report
Fri 17 Jun 2016
SRR1526588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195899
Sequences flagged as poor quality0
Sequence length64
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36771.876987631381477No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28601.45993598742209No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12710.6488037202844323No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.40888416990387905No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.2894348618420717No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC5360.27361038085952455Illumina Single End Adapter 2 (96% over 31bp)
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.2542126299776926No Hit
GGACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.20878105554392826No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.18734143614821924No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.18274723199199588No Hit
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.1715169551656721No Hit
GGACATGGGGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.16539134962404095No Hit
GGACATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3170.16181807972475612No Hit
GGACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.14854593438455532No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.14803546725608605No Hit
GGACATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2830.14446219735680121No Hit
GGACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.14139939458598563No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2530.12914818350272333Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.11995977519027662No Hit
GGACATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11281323539170694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACC201.9487803E-458.3416733
AGACGTT150.00457376958.3416736
GACGTTC150.004592481558.2815637
ACCTTTG201.9587413E-458.28155537
TCGAGCG201.9863436E-458.11687532
CCTTCTA201.9863436E-458.11687531
AACCGTA201.988868E-458.1019527
ACCTGAA150.004648961658.1019530
AAACGTA150.004648961658.1019527
CAAATTC150.004648961658.1019527
CCAGTCA150.004648961658.1019527
AACGTAT150.004653691358.08703628
ATTAGCC150.004653691358.08703628
CCCAGTC150.00466316258.05722426
AAAACGT150.00466316258.05722426
AGAAATT150.00466316258.05722452
AAACCGT201.996457E-458.0572226
AATTCCG258.605435E-658.0423329
AGTCATA150.00466790358.04232829
CAACATC201.9989918E-458.04232824
Produced by FastQC (version 0.11.5)