##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526588_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 195899 Sequences flagged as poor quality 0 Sequence length 64 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.850800667691004 39.0 38.0 40.0 34.0 40.0 2 37.39095656435204 39.0 38.0 40.0 33.0 40.0 3 36.62459736905242 39.0 36.0 39.0 31.0 40.0 4 37.415811208837205 39.0 38.0 40.0 33.0 40.0 5 37.401038290139304 39.0 38.0 40.0 33.0 40.0 6 37.381630329914906 39.0 38.0 40.0 33.0 40.0 7 37.509936242655655 39.0 38.0 40.0 33.0 40.0 8 37.379399588563494 39.0 38.0 40.0 33.0 40.0 9 37.40504545709779 39.0 38.0 40.0 33.0 40.0 10 36.46344289659467 39.0 36.0 40.0 30.0 40.0 11 36.711096023971535 39.0 36.0 40.0 32.0 40.0 12 36.52697563540396 39.0 36.0 40.0 31.0 40.0 13 36.39041036452458 39.0 35.0 40.0 30.0 40.0 14 36.3633913394147 38.0 36.0 40.0 31.0 40.0 15 36.15930658145269 38.0 36.0 40.0 30.0 40.0 16 35.712234365668024 38.0 35.0 40.0 29.0 40.0 17 35.64957452564842 38.0 35.0 40.0 28.0 40.0 18 35.915068479165285 38.0 35.0 40.0 29.0 40.0 19 35.499384887110196 38.0 35.0 39.0 29.0 40.0 20 35.78038683198995 38.0 35.0 40.0 29.0 40.0 21 35.729248235059906 38.0 35.0 40.0 29.0 40.0 22 35.60056457664409 38.0 35.0 40.0 29.0 40.0 23 35.38094119929147 38.0 35.0 39.0 28.0 40.0 24 35.264161634311556 38.0 35.0 39.0 28.0 40.0 25 35.21032777094319 38.0 35.0 39.0 27.0 40.0 26 35.30212507465582 39.0 35.0 40.0 28.0 40.0 27 34.83852393325132 39.0 35.0 40.0 25.0 40.0 28 34.64299970903374 38.0 35.0 40.0 24.0 40.0 29 34.57333115534025 38.0 35.0 40.0 24.0 40.0 30 34.34144125289052 38.0 34.0 39.0 23.0 40.0 31 34.008141950699084 38.0 34.0 39.0 20.0 40.0 32 33.83387357771096 38.0 33.0 39.0 21.0 40.0 33 33.98008667731841 38.0 34.0 39.0 21.0 40.0 34 33.42636766905395 38.0 33.0 39.0 16.0 40.0 35 33.31655598037764 38.0 33.0 39.0 16.0 40.0 36 32.87295494106657 38.0 33.0 39.0 4.0 40.0 37 32.757487276606824 38.0 32.0 39.0 4.0 40.0 38 32.77067264253518 38.0 32.0 39.0 4.0 40.0 39 32.2731866931429 38.0 32.0 39.0 4.0 40.0 40 32.414157295340964 38.0 32.0 39.0 4.0 40.0 41 32.296902995931575 38.0 32.0 39.0 4.0 40.0 42 31.99673811504908 38.0 32.0 39.0 4.0 40.0 43 31.992485923868934 38.0 32.0 39.0 4.0 40.0 44 31.810606485995333 38.0 31.0 39.0 4.0 40.0 45 31.62876278082073 37.0 31.0 39.0 4.0 40.0 46 31.384432794450202 38.0 31.0 39.0 4.0 40.0 47 31.193946880790612 37.0 31.0 39.0 4.0 40.0 48 30.994063267295903 37.0 31.0 39.0 4.0 40.0 49 30.670238235008856 37.0 30.0 39.0 4.0 40.0 50 30.228719901581936 36.0 29.0 39.0 4.0 40.0 51 29.647956344851174 36.0 27.0 39.0 4.0 40.0 52 29.48877227550932 36.0 26.0 39.0 4.0 40.0 53 29.137341180914657 35.0 24.0 39.0 4.0 40.0 54 28.72913593229164 35.0 22.0 39.0 4.0 40.0 55 28.223548869570543 35.0 16.0 39.0 4.0 40.0 56 27.246351436199266 35.0 4.0 39.0 4.0 40.0 57 26.776155059494943 35.0 4.0 39.0 4.0 40.0 58 26.45434637236535 34.0 4.0 39.0 4.0 40.0 59 26.033706144492825 34.0 4.0 39.0 4.0 40.0 60 25.558573550656206 34.0 4.0 39.0 4.0 40.0 61 24.961010520727516 34.0 4.0 39.0 4.0 40.0 62 24.70955441324356 33.0 4.0 39.0 4.0 40.0 63 24.34012424769907 33.0 4.0 39.0 4.0 40.0 64 24.302610018427863 33.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.282881441045177 2 2 0.47597261388631296 2 3 0.04924663618148628 2 4 0.42795232807415573 2 5 0.1571477547584692 2 6 0.2769216143135509 2 7 0.14443733102652345 2 8 0.43341115372989236 2 9 -0.14094181753293356 2 10 0.4580554421788463 2 11 0.0028856260923717514 2 12 0.17127890229118492 2 13 0.5403197878190369 2 14 0.18121205285148534 2 15 0.5263913394990851 2 16 0.0557841453175385 2 17 0.6615392262768438 2 18 0.41037516766711946 2 19 -6.356147110793131E-4 2 20 0.8335226720685753 2 21 0.8363281506300595 2 22 0.705311447362817 2 23 0.6376311453787622 2 24 0.950933620417139 2 25 1.083606078451922 2 26 1.6485623374923577 2 27 2.139996997912121 2 28 1.7346459283170788 2 29 2.216411167258535 2 30 1.7722128327609354 2 31 1.595016923057436 2 32 1.1081831592228895 2 33 0.4179761328196818 2 34 1.2363554981426432 2 35 1.19200454619007 2 36 0.11114653210614733 2 37 -0.2025920939448156 2 38 0.05651393115588377 2 39 -4.397174401092833 2 40 -0.6654815960423122 2 41 -1.4567111693736159 2 42 -1.46768449228151 2 43 -1.734892757046726 2 44 -0.8979278593262627 2 45 -1.3862593416023827 2 46 -0.352560716635665 2 47 -0.5338027131129905 2 48 -0.3643834328722839 2 49 -0.4941249807769701 2 50 -0.23563583851811387 2 51 -0.6519750543516167 2 52 -0.6860451295625545 2 53 -0.4586274856012338 2 54 -0.27504499534180127 2 55 -0.4228566408036585 2 56 0.14510838283878513 2 57 -0.06268307957483543 2 58 0.0033323408086687323 2 59 0.2870496741848747 2 60 0.08358797941933105 2 61 0.3553474891174382 2 62 -0.006633350378592695 2 63 0.007491886790571556 2 64 0.2964522446710305 3 1 0.10733899083932386 3 2 0.566681415488631 3 3 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0.3227098777329047 4 20 0.11643517384506907 4 21 -0.42960107387133917 4 22 -0.8601582575024054 4 23 -0.5607202687100141 4 24 -0.29045630804939293 4 25 -0.11552699274993472 4 26 0.05862439632981875 4 27 0.45353624657382596 4 28 0.2578114027592662 4 29 0.5356825680828265 4 30 0.3981543742835072 4 31 -0.178634126258018 4 32 -0.6634308442827219 4 33 -0.7190744143402981 4 34 -0.17226167936458125 4 35 -5.243212659741051 4 36 -0.9705247929210934 4 37 -1.3975326406310593 4 38 -1.3715499278615937 4 39 -1.070110115716929 4 40 -0.9958856890831953 4 41 -0.31200985713712726 4 42 -0.34233511523854077 4 43 -0.46569478461790226 4 44 -0.12531901949490987 4 45 -0.3769742025621632 4 46 -0.12450633224985808 4 47 -0.012745344218206611 4 48 0.013275385641147608 4 49 -0.39954447009432315 4 50 -0.10329892061579571 4 51 0.23283711107545457 4 52 0.10847379601421281 4 53 -0.20323878883682056 4 54 0.3534073235742987 4 55 0.5728087152056283 4 56 0.31828096325471833 4 57 0.46730412967240653 4 58 0.4154551320351594 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119 9 0.3974919782401898 119 10 -0.17758688428600777 119 11 0.11192363912403636 119 12 0.011580890702134639 119 13 0.2165237742584054 119 14 0.12030945373148683 119 15 -0.17960274575974466 119 16 -0.8861263641676871 119 17 0.018635713497637596 119 18 -0.34428329209580966 119 19 -0.6702997247348819 119 20 -0.32478080704949974 119 21 -0.48181760205682167 119 22 -0.7894830995343511 119 23 -0.6543070143192509 119 24 -0.6381601739133629 119 25 -0.8684749006060457 119 26 -0.3827905994411154 119 27 -0.08875441465309564 119 28 -0.34662165361169883 119 29 -0.1411421495648142 119 30 -0.3732272864956343 119 31 -0.14997598406188928 119 32 -0.5642219284162664 119 33 -0.4074668716764833 119 34 0.22529541302807132 119 35 0.20123731289439917 119 36 0.1700294635095716 119 37 -0.04378369789758807 119 38 -0.05740494552416209 119 39 0.5430049646353297 119 40 0.48784484477420875 119 41 -0.025055282282657743 119 42 0.05076901929551525 119 43 -0.07963080133515277 119 44 -0.3848841719900591 119 45 -0.29741800573644994 119 46 0.16550809632867924 119 47 0.21532570409265617 119 48 0.3146336621723158 119 49 -0.06408001912661376 119 50 0.16947584429788165 119 51 -0.8800292006813493 119 52 -0.757166285581885 119 53 -0.5563409332221134 119 54 -0.37448958671974353 119 55 0.26719649569221815 119 56 6.780974404989593E-4 119 57 -0.18215604247546224 119 58 -0.16932520529505624 119 59 -0.3161337068350605 119 60 -0.6599863515657383 119 61 -0.9607064648629908 119 62 -0.90454395603847 119 63 -0.6222597421676177 119 64 -1.5186760282726333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 715.0 5 60.0 6 204.0 7 310.0 8 928.0 9 604.0 10 784.0 11 817.0 12 787.0 13 1095.0 14 1127.0 15 1289.0 16 1554.0 17 1779.0 18 1828.0 19 2049.0 20 2141.0 21 2592.0 22 2675.0 23 2196.0 24 2326.0 25 2905.0 26 3086.0 27 3600.0 28 4572.0 29 5344.0 30 6511.0 31 7579.0 32 8620.0 33 10338.0 34 11724.0 35 12759.0 36 15250.0 37 20152.0 38 28146.0 39 27330.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.34812682174817 0.32006615721527126 0.18479098710036396 0.14701603393620116 8 98.42991036085502 0.902009857752126 0.4132083663201982 0.25487141507265626 9 95.19956712387506 2.7473340854215693 0.9566153987513974 1.0964833919519752 10 34.216611621294646 43.81645643928759 8.860688415969452 13.106243523448308 11 22.255856334131362 41.876681351104395 23.46770529711739 12.399757017646849 12 53.23151215677466 20.21858202441054 15.263988075487877 11.28591774332692 13 25.33142078315867 18.36303401242477 24.788794225595844 31.516750978820717 14 28.875083588992283 37.5106560013068 18.369670085094867 15.244590324606047 15 42.911818660289526 23.8141227088539 17.635756953280293 15.638301677576278 16 21.741283879005742 25.68013316721472 31.200343130246523 21.378239823533015 17 34.62753557991166 25.408453296253885 16.643832815311633 23.32017830852282 18 32.64488333273779 29.584122430436093 18.998565587369 18.77242864945712 19 20.467689983103536 28.483555301456363 33.04815236422851 18.000602351211594 20 22.84795261921781 36.81456142142346 17.05299703870111 23.284488920657612 21 24.350302962240747 30.225779610921954 27.635158933940453 17.78875849289685 22 24.24412579951914 32.151772086636484 15.889820774991195 27.714281338853187 23 27.323773985574203 40.37743939479017 16.083287816681043 16.215498802954585 24 19.53024896498563 46.936816903277844 18.464350072234048 15.068584059502479 25 21.202572051665754 28.83292389566745 23.82314335766125 26.141360695005538 26 30.2426272363369 28.99170819377502 21.056286251123275 19.709378318764806 27 19.829900835153143 28.508231347138334 22.230007211059004 29.43186060664952 28 20.626872080066246 38.97095656263098 23.08651693433791 17.31565442296486 29 27.563879803761242 31.847403924775143 24.392375306623055 16.196340964840555 30 16.69453948478758 44.46302632319172 26.577371363108664 12.26506282891204 31 23.92982276668116 36.64919055778624 28.287256080407154 11.133730595125444 32 13.685652789635938 37.254791691164066 38.68935935255293 10.37019616664707 33 18.002168997657073 37.65103692411578 30.221708392588575 14.125085685638576 34 16.40030538888405 38.923247984997 31.59442716526355 13.082019460855395 35 12.745489341234023 46.88782454739901 31.516277260958113 8.85040885040885 36 14.924295197508606 40.133277331584985 31.996803802655304 12.945623668251105 37 12.46395074249945 39.69080878398107 37.61467889908257 10.230561574436914 38 13.934799866833977 39.47553074342493 36.94435196804016 9.64531742170094 39 12.564826093884118 38.968641902970305 35.91404149897974 12.552490504165831 40 13.170836663183131 39.602672076391876 38.07811315341898 9.148378107006003 41 13.2119863856311 40.96561551709998 36.255433445419506 9.566964651849421 42 13.63223545469006 40.00338650835595 35.789749141828 10.574628895125993 43 12.774919069776985 41.29006110167709 36.30342549032685 9.631594338219074 44 11.647154746703999 40.420698531862634 37.31776482111082 10.614381900322549 45 11.890128156455535 42.55327881468104 36.010014467622284 9.546578561241137 46 11.553717396770336 40.73665467644357 37.16165249562554 10.547975431160554 47 13.39073588497596 40.72998393891657 36.16771260410199 9.71156757200548 48 14.252656531398635 41.105814866314 34.9769365357086 9.664592066578757 49 14.271785371561116 41.89985990444765 34.583074609862116 9.245280114129125 50 11.683801127226582 42.877253554051904 35.59600692571299 9.842938393008524 51 11.160338419204468 43.929297492658684 35.681869519682934 9.22849456845391 52 12.852323318424796 42.49395356957631 34.62255382735195 10.031169284646944 53 12.278953666942307 42.562108690851005 34.227705329765435 10.931232312441255 54 12.501861542332756 43.13283452557631 33.452300866323284 10.913003065767649 55 13.386073510903346 42.93786761872961 32.42031181773817 11.255747052628875 56 13.604229389944408 42.76914066112475 32.57380367656881 11.052826272362026 57 13.919257721789524 42.962536733729266 32.23988409609338 10.878321448387826 58 13.953488372093023 42.95128821993296 31.412312627228424 11.682910780745583 59 12.81661105998838 43.281702232663825 32.475671007243434 11.426015700104358 60 13.4981409006989 43.68549079707276 30.851791472314076 11.964576829914272 61 13.917976015537493 42.70138624849712 31.440814690740186 11.9398230452252 62 13.231885622073872 42.95133696160507 29.965104865276 13.851672551045056 63 13.89108737884036 45.24869204123796 28.553073831573972 12.307146748347707 64 14.695821695497635 44.94647881528119 27.844389920219744 12.513309569001436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 23.5 5 78.5 6 110.0 7 193.5 8 277.0 9 1103.0 10 1773.5 11 1618.0 12 1450.5 13 1199.5 14 1116.0 15 1506.5 16 2405.5 17 2914.0 18 3351.5 19 3789.0 20 4512.0 21 5897.0 22 6559.0 23 7067.0 24 8202.0 25 8829.0 26 9722.5 27 11472.0 28 12328.0 29 10592.5 30 9863.5 31 10870.0 32 9017.5 33 7165.0 34 6846.5 35 6426.5 36 6325.0 37 6226.0 38 5976.5 39 5826.0 40 5580.5 41 5105.0 42 4875.0 43 4802.0 44 4729.0 45 4766.0 46 4728.0 47 4653.0 48 4521.0 49 4296.0 50 4203.0 51 4419.5 52 4924.0 53 5212.0 54 6017.0 55 6717.5 56 6613.0 57 5726.5 58 4840.0 59 4562.5 60 3964.5 61 3644.0 62 3236.5 63 2411.0 64 1993.0 65 1732.0 66 1208.0 67 945.0 68 782.0 69 619.0 70 510.0 71 332.5 72 264.0 73 240.0 74 163.5 75 111.0 76 94.5 77 63.5 78 49.0 79 35.5 80 23.5 81 25.0 82 16.5 83 8.0 84 5.5 85 3.5 86 4.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0010209342569385245 8 0.058193252645495894 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.10209342569385245 16 0.02807569206580942 17 0.14446219735680121 18 0.0 19 0.0 20 0.019908218010301227 21 0.0 22 0.0 23 0.0 24 0.004083737027754098 25 0.05206764710386475 26 0.021950086524178274 27 0.18683096901974997 28 0.13323192053047744 29 0.11179230113476843 30 0.1878519032766885 31 0.2011240486168893 32 0.15314013854077865 33 0.21286479257168234 34 0.3767247408103155 35 0.24145095176596104 36 0.34048157468899787 37 0.34711764735909834 38 0.33384550201889746 39 0.6840259521488113 40 0.35477465428613725 41 0.4139888411885717 42 0.5145508654970163 43 0.4992368516429384 44 0.4543157443376434 45 0.5007682530283463 46 0.519655536781709 47 0.519655536781709 48 0.5114880627262007 49 0.5268020765802786 50 0.644209516128209 51 0.5671289797293503 52 0.5901000005104671 53 0.6125605541631146 54 0.5962256060520983 55 0.6299164365310697 56 0.6436990489997397 57 0.6406362462289241 58 0.6978085646174814 59 0.7034237030306433 60 0.7401773362804303 61 0.6498246545413708 62 0.6717747410655491 63 0.6743270767078954 64 0.7605960214192007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 195899.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.492377276726536 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6426432584487 49.166073118775515 2 9.357189475936513 11.133628931044603 3 3.42295313852947 6.109188585538513 4 1.5722979482877038 3.7415897092382058 5 0.8249704898805807 2.453972781307071 6 0.45378047950440004 1.6197887692493786 7 0.32858124681382894 1.3683655651053859 8 0.21936959077221668 1.0440654765809458 9 0.1876021388349967 1.004480749934321 >10 0.8794413560299931 8.893817916844686 >50 0.05260764713361656 2.1164223214029887 >100 0.05260764713361656 5.613479516533708 >500 0.002977791347185843 1.1243493582453377 >1k 0.002977791347185843 4.610777200199368 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3677 1.876987631381477 No Hit GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2860 1.45993598742209 No Hit GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1271 0.6488037202844323 No Hit GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 801 0.40888416990387905 No Hit GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 567 0.2894348618420717 No Hit GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC 536 0.27361038085952455 Illumina Single End Adapter 2 (96% over 31bp) GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 498 0.2542126299776926 No Hit GGACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 409 0.20878105554392826 No Hit GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.18734143614821924 No Hit GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 358 0.18274723199199588 No Hit GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 336 0.1715169551656721 No Hit GGACATGGGGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 324 0.16539134962404095 No Hit GGACATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 317 0.16181807972475612 No Hit GGACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 291 0.14854593438455532 No Hit GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 290 0.14803546725608605 No Hit GGACATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 283 0.14446219735680121 No Hit GGACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 277 0.14139939458598563 No Hit GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA 253 0.12914818350272333 Illumina Single End Adapter 2 (97% over 34bp) GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 235 0.11995977519027662 No Hit GGACATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.11281323539170694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.104671284692623E-4 0.0 0.0 0.0 0.0 8 0.006125605541631146 0.0 0.0 0.0 0.0 9 0.010719809697854507 0.0 0.0 0.0 0.0 10 0.07503866788498155 0.0 0.0 0.0 0.0 11 0.13833659181517005 0.0 0.0 0.0 0.0 12 0.14446219735680121 0.0 0.0 0.0 0.0 13 0.15109827002690163 0.0 0.0 0.0 0.0 14 0.20622871990158193 0.0 0.0 0.0 0.0 15 0.22001133237025203 0.0 0.0 0.0 0.0 16 0.23430441196739135 0.0 0.0 0.0 0.0 17 0.24706609017912293 0.0 0.0 0.0 0.0 18 0.26186963690473153 0.0 0.0 0.0 0.0 19 0.27616271650187085 0.0 0.0 0.0 0.0 20 0.3404815746889979 0.0 0.0 0.0 0.0 21 0.36141072695623766 0.0 0.0 0.0 0.0 22 0.4216458481156106 0.0 0.0 0.0 0.0 23 0.4701402253201905 0.0 0.0 0.0 0.0 24 0.5160822668824241 0.0 0.0 0.0 0.0 25 0.5426265575628257 0.0 0.0 0.0 0.0 26 0.5635557098300655 0.0 0.0 0.0 0.0 27 0.5875476648681208 0.0 0.0 0.0 0.0 28 0.7483448103359385 0.0 0.0 0.0 0.0 29 0.7692739626031782 0.0 0.0 0.0 0.0 30 0.8361451564326515 0.0 0.0 0.0 0.0 31 0.8540115059290757 0.0 0.0 0.0 0.0 32 0.8749406581963155 0.0 0.0 0.0 0.0 33 0.8917860734358011 0.0 0.0 0.0 0.0 34 0.9162884956023257 0.0 0.0 0.0 0.0 35 1.142935900642678 0.0 0.0 0.0 0.0 36 1.167948789937672 0.0 0.0 0.0 0.0 37 1.189388409333381 0.0 0.0 0.0 0.0 38 1.2200164370415367 0.0 0.0 0.0 0.0 39 1.2511549318781616 0.0 0.0 0.0 0.0 40 1.280762025329379 0.0 0.0 0.0 0.0 41 1.313942388679881 0.0 0.0 0.0 0.0 42 1.3425285478741595 0.0 0.0 0.0 0.0 43 1.3797926482524159 0.0 0.0 0.0 0.0 44 1.4145044129883255 0.0 0.0 0.0 0.0 45 1.444111506439543 0.0 0.0 0.0 0.0 46 1.4767814026615755 0.0 0.0 0.0 0.0 47 1.5089408317551392 0.0 0.0 0.0 0.0 48 1.5390583923348256 0.0 0.0 0.0 0.0 49 1.5661131501436965 0.0 0.0 0.0 0.0 50 1.5870423024109364 0.0 0.0 0.0 0.0 51 1.607971454678176 0.0 0.0 0.0 0.0 52 1.6243064027891925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTACC 20 1.9487803E-4 58.34167 33 AGACGTT 15 0.004573769 58.34167 36 GACGTTC 15 0.0045924815 58.28156 37 ACCTTTG 20 1.9587413E-4 58.281555 37 TCGAGCG 20 1.9863436E-4 58.116875 32 CCTTCTA 20 1.9863436E-4 58.116875 31 AACCGTA 20 1.988868E-4 58.10195 27 ACCTGAA 15 0.0046489616 58.10195 30 AAACGTA 15 0.0046489616 58.10195 27 CAAATTC 15 0.0046489616 58.10195 27 CCAGTCA 15 0.0046489616 58.10195 27 AACGTAT 15 0.0046536913 58.087036 28 ATTAGCC 15 0.0046536913 58.087036 28 CCCAGTC 15 0.004663162 58.057224 26 AAAACGT 15 0.004663162 58.057224 26 AGAAATT 15 0.004663162 58.057224 52 AAACCGT 20 1.996457E-4 58.05722 26 AATTCCG 25 8.605435E-6 58.04233 29 AGTCATA 15 0.004667903 58.042328 29 CAACATC 20 1.9989918E-4 58.042328 24 >>END_MODULE