SRR1526587_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169110
Sequences flagged as poor quality	0
Sequence length	56
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	4842	2.863225119744545	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	4007	2.3694636627047485	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1232	0.7285198982910531	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805	0.47602152445154045	No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.3749039086984803	No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	555	0.3281887528827391	No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	461	0.27260363077286975	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	459	0.2714209686003193	No Hit
GGACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.2513157116669623	No Hit
GGACATGGGGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.22293181952575247	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	370	0.21879250192182606	Illumina Single End Adapter 2 (95% over 23bp)
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.2152445154041748	No Hit
GGACATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	344	0.2034178936786707	No Hit
GGACATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	308	0.18212997457276328	No Hit
GGACATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.17858198805511205	No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.17680799479628642	No Hit
GGACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.1649813730707823	No Hit
GGACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	271	0.1602507243805807	No Hit
GGACATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.14724144048252616	No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	241	0.1425107917923245	Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.13009283898054522	No Hit
GGACATGGGGAGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	209	0.12358819703151794	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGT	207	0.12240553485896753	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	190	0.11235290639228905	No Hit
GGACATGGGGTGCTGGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186	0.10998758204718823	No Hit
GGACATGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.10821358878836261	No Hit
GGACATGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.10584826444326179	No Hit
GGACATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.10407427118443616	No Hit
GGACATGGGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	175	0.10348294009816096	No Hit
GGACATGGGAGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	173	0.10230027792561054	No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	170	0.10052628466678494	Illumina Single End Adapter 2 (97% over 34bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGC	20	3.8938224E-4	50.707783	37
AGCCACA	15	0.008010999	50.631435	35
TGCATTA	15	0.008010999	50.631435	35
TGCGATT	15	0.008010999	50.631435	36
TTGCGAT	15	0.008010999	50.631435	35
ATAACGG	15	0.008010999	50.631435	36
CATATGC	20	3.9933805E-4	50.449142	33
GCGGTCG	15	0.008126079	50.449142	33
TGGAGGG	15	0.008135724	50.43401	32
AGCCATA	15	0.008135724	50.43401	32
CCGGCCG	15	0.008261888	50.238125	39
ACCAGCT	15	0.008261888	50.238125	38
GCACGGG	15	0.008271653	50.223114	43
GTTATGG	15	0.008300999	50.178154	42
ACCGCGA	25	2.0983987E-5	49.92488	28
CCGCGAT	25	2.0983987E-5	49.92488	29
CACGTAT	20	4.2047224E-4	49.92488	28
ATCTAAA	15	0.008468758	49.924877	28
ACACGTA	15	0.008478704	49.91006	27
CACATTC	15	0.008478704	49.91006	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content