##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169110
Sequences flagged as poor quality	0
Sequence length	56
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.04296020341789	39.0	38.0	40.0	35.0	40.0
2	37.60655786174679	39.0	38.0	40.0	33.0	40.0
3	36.73441546922122	39.0	36.0	40.0	31.0	40.0
4	37.69382059014843	39.0	38.0	40.0	33.0	40.0
5	37.35210809532257	39.0	38.0	40.0	33.0	40.0
6	37.60273786292945	39.0	38.0	40.0	33.0	40.0
7	37.664259949145524	39.0	38.0	40.0	35.0	40.0
8	37.865365738276864	39.0	38.0	40.0	35.0	40.0
9	37.71431021228786	39.0	38.0	40.0	34.0	40.0
10	36.6246703329194	39.0	36.0	40.0	31.0	40.0
11	36.995996688545915	39.0	36.0	40.0	33.0	40.0
12	36.93909289811366	39.0	36.0	40.0	32.0	40.0
13	36.848004257583824	39.0	36.0	40.0	32.0	40.0
14	36.81583584649045	39.0	36.0	40.0	32.0	40.0
15	36.8398793684584	39.0	36.0	40.0	32.0	40.0
16	36.51265448524629	39.0	36.0	40.0	31.0	40.0
17	36.687292294955945	39.0	36.0	40.0	32.0	40.0
18	36.57632310330554	39.0	36.0	40.0	31.0	40.0
19	36.0881733782745	38.0	35.0	40.0	30.0	40.0
20	36.42811779315239	39.0	36.0	40.0	31.0	40.0
21	36.20652829519248	39.0	36.0	40.0	30.0	40.0
22	36.10198095913902	39.0	36.0	40.0	30.0	40.0
23	35.834037017326004	38.0	35.0	40.0	29.0	40.0
24	35.727514635444386	38.0	35.0	40.0	29.0	40.0
25	35.691502572290226	38.0	35.0	40.0	29.0	40.0
26	35.29933770918337	38.0	35.0	40.0	28.0	40.0
27	35.2952693513098	38.0	35.0	40.0	28.0	40.0
28	34.91192714801017	38.0	35.0	40.0	27.0	40.0
29	34.92342262432736	38.0	35.0	40.0	27.0	40.0
30	34.487700313405476	38.0	35.0	40.0	25.0	40.0
31	33.9073502454024	38.0	34.0	40.0	20.0	40.0
32	33.40456507598604	38.0	33.0	39.0	16.0	40.0
33	33.653899828513985	38.0	33.0	40.0	17.0	40.0
34	33.60918337176985	38.0	33.0	40.0	16.0	40.0
35	33.22727810300987	38.0	33.0	40.0	9.0	40.0
36	32.833209153805214	38.0	33.0	40.0	4.0	40.0
37	32.436603394240436	38.0	33.0	40.0	4.0	40.0
38	32.4979835609958	38.0	32.0	40.0	4.0	40.0
39	32.41226420672935	38.0	32.0	39.0	4.0	40.0
40	32.0236236768967	38.0	32.0	39.0	4.0	40.0
41	31.826272840163206	38.0	32.0	39.0	4.0	40.0
42	31.709816096032167	38.0	32.0	39.0	4.0	40.0
43	31.570468925551417	38.0	31.0	39.0	4.0	40.0
44	31.0774407190586	37.0	30.0	39.0	4.0	40.0
45	31.086795576843475	37.0	31.0	39.0	4.0	40.0
46	30.66472118739282	37.0	30.0	39.0	4.0	40.0
47	30.448228963396605	37.0	30.0	39.0	4.0	40.0
48	30.013807580864526	37.0	28.0	39.0	4.0	40.0
49	29.85379930222932	36.0	28.0	39.0	4.0	40.0
50	29.489332387203596	36.0	27.0	39.0	4.0	40.0
51	28.725232097451364	36.0	21.0	39.0	4.0	40.0
52	28.500118266217257	35.0	15.0	39.0	4.0	40.0
53	28.068706758914317	35.0	4.0	39.0	4.0	40.0
54	27.584790964461003	35.0	4.0	39.0	4.0	40.0
55	26.937809709656438	35.0	4.0	39.0	4.0	40.0
56	15.6029211755662	19.0	4.0	22.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.20384120112245796
1	2	-0.5050874574967636
1	3	-0.5446628412687318
1	4	-0.7019436640237444
1	5	-0.2903628709755921
1	6	-0.6596134996554639
1	7	-0.8689013065580511
1	8	-1.2361142556507474
1	9	-1.5011858729797467
1	10	-0.22539166597236004
1	11	-1.0187711201383536
1	12	-1.0109724330667547
1	13	-1.1377649929540183
1	14	-1.4135319055934161
1	15	-0.9189873688812824
1	16	-1.237290083334024
1	17	-1.1851616428803027
1	18	-1.358411624347255
1	19	-1.5448144272025388
1	20	-1.6805976188542218
1	21	-0.9710627179031945
1	22	-0.9941199846964963
1	23	-0.7255714585879574
1	24	-0.8464179311521463
1	25	-0.7156745331502634
1	26	-0.6265268687546452
1	27	-0.6497669911142836
1	28	-0.2502310439081654
1	29	-0.484546696838855
1	30	-1.1646432940149012
1	31	-0.7683506266764226
1	32	-1.5253793960548805
1	33	-1.3216217988377892
1	34	-1.4215346755947493
1	35	-1.7545740339025073
1	36	-1.8466840524288095
1	37	-2.0165567165465177
1	38	-2.0646117038977536
1	39	-2.3123529825276776
1	40	-2.027385871141856
1	41	-1.5242878320418107
1	42	-1.5617633716456574
1	43	-1.6959308026575641
1	44	-1.6650411991742118
1	45	-2.113783228977578
1	46	-2.656218481974019
1	47	-2.337993118404537
1	48	-2.06716515760413
1	49	-2.2592135780901508
1	50	-2.8077966238979144
1	51	-2.7512393825642256
1	52	-2.505292655847036
1	53	-2.1364746327690582
1	54	-2.020532434072866
1	55	-1.7176823256206646
1	56	-0.6535017780538919
2	1	-0.23705127081382216
2	2	0.41832315265826736
2	3	0.2714372867358179
2	4	0.25631262953335465
2	5	0.5908062372593648
2	6	-0.020870788803520668
2	7	0.4575102991879305
2	8	0.2338046748001119
2	9	0.22495550093577066
2	10	-0.30418557864481244
2	11	-1.0740975317440942
2	12	-1.0339421386562861
2	13	-1.0950968091963773
2	14	-0.9722717718997771
2	15	-1.3316598194573075
2	16	-1.5149177323171017
2	17	-1.1190259580559498
2	18	-1.0593787698457646
2	19	-1.6606585461045427
2	20	-1.4308465165928084
2	21	-1.8476805532040075
2	22	-1.8004064304367873
2	23	-1.4917498115960655
2	24	-1.6699138776746878
2	25	-1.9879686591636343
2	26	-1.4293287461547308
2	27	-1.2692379056356842
2	28	-1.8163667287325183
2	29	-1.8270299851335494
2	30	-1.999872991070795
2	31	-1.6341591887586304
2	32	-2.4807200303885466
2	33	-2.298652093248254
2	34	-2.0690243641170056
2	35	-2.0913682310295165
2	36	-1.5690564034457353
2	37	-1.5450732860245466
2	38	-1.2327243479028738
2	39	-1.2673380486633619
2	40	-0.955489981510226
2	41	-0.40709255398747857
2	42	-0.48090716342492
2	43	-0.46658931496022404
2	44	-0.25876897616183925
2	45	-0.82136394295852
2	46	-0.9676962234048254
2	47	-0.3947418028022014
2	48	-0.45722347078859826
2	49	-0.61008827585718
2	50	-0.7367540979414358
2	51	-1.1757166973131952
2	52	-1.2388298482805453
2	53	-0.7021125221162343
2	54	-1.0596449358507058
2	55	-0.5632235004179904
2	56	-0.4117865170668349
3	1	-0.4036276888455035
3	2	0.28254408131672193
3	3	-0.384986159859551
3	4	-0.5017759043775669
3	5	0.05384942123120595
3	6	-0.26034554174788127
3	7	-0.18672958168387055
3	8	-0.15985987102363453
3	9	-0.6090095728424885
3	10	-0.6278623080342811
3	11	-0.6882846171644275
3	12	-0.5039570296810609
3	13	-0.3664518924507405
3	14	-0.6640275590934976
3	15	-1.073097480212688
3	16	-1.0527544725364066
3	17	-0.8736624816785294
3	18	-1.0614770506092697
3	19	-1.3089596155508687
3	20	-1.5385357002938989
3	21	-1.9964554279840243
3	22	-2.2146476650381146
3	23	-1.4855226557818
3	24	-2.097218602190729
3	25	-2.064995869127088
3	26	-1.745559963157568
3	27	-2.0865521062584023
3	28	-1.3810073135436696
3	29	-1.6218045891676596
3	30	-1.7518020556436937
3	31	-1.1758540581237327
3	32	-0.9387544151184812
3	33	-1.4310468407776966
3	34	-1.1450362769368283
3	35	-1.5460182919473446
3	36	-1.4890509885276373
3	37	-1.4589084017684186
3	38	-1.5740672475915147
3	39	-1.755695974749731
3	40	-1.5845004052275655
3	41	-1.4065510621775417
3	42	-1.389733479926882
3	43	-1.8806951766090663
3	44	-1.2546401011110753
3	45	-1.1220949569019325
3	46	-2.0368041156479535
3	47	-2.170767252917269
3	48	-1.656154024463973
3	49	-1.5940465809878148
3	50	-1.4303374199936911
3	51	-1.5453525440710152
3	52	-1.350038728746231
3	53	-1.1778807636215838
3	54	-1.0734529770040844
3	55	-0.9116734801120465
3	56	-0.8775371600883588
4	1	-0.07929587884688516
4	2	0.6529221452967775
4	3	0.19104209107521797
4	4	0.21446838311724292
4	5	0.5962950658787634
4	6	0.016001224627963495
4	7	0.09363324894900416
4	8	0.12777342076420695
4	9	0.0172194813051334
4	10	-0.15424369565372587
4	11	-0.30198078113063787
4	12	-0.7332189047676181
4	13	-0.5741101115339688
4	14	-0.6730983249154008
4	15	-0.8567147077434996
4	16	-0.3851876535362635
4	17	-0.6137954272539332
4	18	-0.18265140319201123
4	19	-0.393643177384412
4	20	-0.8750541880379785
4	21	-0.3815821417355849
4	22	-0.7597245241988944
4	23	-0.23306454748654915
4	24	-0.4284054184211712
4	25	-0.07185087535891199
4	26	-0.4111187500291962
4	27	-0.23029950966513013
4	28	0.17840274046546511
4	29	-0.07977439944324516
4	30	-0.18726805758248588
4	31	0.057562366194225945
4	32	0.4042728664214721
4	33	0.285682144127442
4	34	0.48534732644220924
4	35	-0.17847916994207935
4	36	-0.026372689123121518
4	37	0.13941593004157937
4	38	0.3253926610084008
4	39	0.3589695840371405
4	40	0.38087835117736546
4	41	0.31530186677966654
4	42	0.6055580309441915
4	43	0.5319900470441681
4	44	0.6812413557508563
4	45	0.3588633591207788
4	46	0.6176066307860566
4	47	0.22820795827231777
4	48	0.5676376941345609
4	49	0.42374873822004844
4	50	0.43540139734462713
4	51	0.7680389529515601
4	52	0.664502193563699
4	53	0.853486083075552
4	54	0.5566536564261888
4	55	0.8489552416716393
4	56	0.37305140105275747
5	1	0.1201013737463299
5	2	0.6827791680049344
5	3	-0.024054628857491878
5	4	-0.10638948063380838
5	5	0.5471641435350563
5	6	-0.05163264136587031
5	7	0.26786930165436473
5	8	-0.01359187219906488
5	9	0.13952113535615496
5	10	-1.2351940741230223
5	11	-0.6611761834294896
5	12	-0.4568662274477404
5	13	-0.7641157184865932
5	14	-1.4619264718175629
5	15	-1.0222599838859168
5	16	-1.24354201294193
5	17	-1.7002125845289555
5	18	-1.8677509266010404
5	19	-2.4099734268937993
5	20	-2.5956176867764142
5	21	-1.8058882813487003
5	22	-2.094459595687539
5	23	-1.738461239384499
5	24	-1.7548001478857032
5	25	-1.88787274247413
5	26	-2.3007318702983284
5	27	-1.743545935232838
5	28	-2.369326443722926
5	29	-1.9119162553445648
5	30	-1.9701388173245675
5	31	-2.595998048720265
5	32	-2.4718134806488905
5	33	-2.5464597117738776
5	34	-2.531753879052605
5	35	-2.47277409564996
5	36	-2.3099443179428576
5	37	-4.728890770266805
5	38	-2.492521552616491
5	39	-2.56659811991576
5	40	-2.2826344046398503
5	41	-2.1117376622363153
5	42	-2.6018992160420744
5	43	-2.5389379036146487
5	44	-1.8199501216811598
5	45	-1.9417393882860061
5	46	-1.2210533075374634
5	47	-1.3650076290559703
5	48	-1.4045378034824871
5	49	-1.484437412606976
5	50	-1.3840547282511118
5	51	-0.6681118740739507
5	52	-0.9971883026516082
5	53	-0.9072372138125893
5	54	-0.38692638282248026
5	55	-0.9320231715607683
5	56	-0.4485774186837155
6	1	-0.0387599338016571
6	2	0.460308393001327
6	3	0.05117267666999936
6	4	-0.5773088052116719
6	5	0.06653923373566073
6	6	-0.43989213046963727
6	7	-0.008216713643662388
6	8	-0.1331257154664911
6	9	-0.09207794098963262
6	10	-0.13139121656782038
6	11	-0.5738980769206137
6	12	-0.13759216192601542
6	13	-0.13079922259974097
6	14	-0.10909778080876009
6	15	-0.1855591612563856
6	16	-0.6921998279224439
6	17	-0.703062923683234
6	18	-1.0091493967987617
6	19	-0.6293773812883714
6	20	-0.6714222761832573
6	21	-0.5371626355123666
6	22	-0.8748030795957504
6	23	-1.0936712263956139
6	24	-1.0364786007649087
6	25	-0.7666070475686624
6	26	-1.1800434592722056
6	27	-1.3018155268044893
6	28	-1.2944599406200226
6	29	-1.4480325780462522
6	30	-1.3245189587591142
6	31	-1.8956444623441655
6	32	-1.653946544583082
6	33	-2.017487277079116
6	34	-2.4815301815493562
6	35	-1.7739358794571451
6	36	-2.1550879173078457
6	37	-2.415028741113968
6	38	-1.8229444130581136
6	39	-1.9776964024962176
6	40	-1.63041186170436
6	41	-1.0960427567665505
6	42	-1.3560484439248874
6	43	-1.4698803195476842
6	44	-1.1004018463167498
6	45	-1.2662811692067741
6	46	-1.866240203193403
6	47	-1.3732339243366738
6	48	-1.1732995295612838
6	49	-1.1204599266782722
6	50	-1.545044770104191
6	51	-1.840034229392181
6	52	-1.6151346152508275
6	53	-2.373610427840589
6	54	-1.6408230490080769
6	55	-1.7015786629736702
6	56	-1.2445436475767728
7	1	0.3213208859414536
7	2	1.0974133143463973
7	3	0.3783843955327484
7	4	0.674098324244234
7	5	1.0532617045106676
7	6	0.5093429683902286
7	7	0.9812839358007039
7	8	0.8034812985326454
7	9	0.639521135356155
7	10	1.0087083649013664
7	11	0.6132140604729486
7	12	0.8358166993815317
7	13	0.6200306229768202
7	14	0.6417320647678011
7	15	0.03871562587018218
7	16	0.2625555480336814
7	17	0.10466546425153211
7	18	-0.09945824367421352
7	19	0.1936851096915646
7	20	-0.2419591501910503
7	21	0.1026483040171513
7	22	-0.07006935178510076
7	23	0.17617290695696397
7	24	0.2208096082118587
7	25	0.3011516477697711
7	26	-0.20317089468857574
7	27	0.317429674523261
7	28	0.19164916603317295
7	29	0.3319861836798239
7	30	0.3713245973095809
7	31	0.4405873171333923
7	32	0.17452798276574555
7	33	-0.022069467871439485
7	34	0.25483148680105216
7	35	-0.08862775418654678
7	36	-0.4745784642843205
7	37	-0.10084198977899916
7	38	-0.04130204042137109
7	39	-0.06659811991575992
7	40	0.2417558392625878
7	41	0.7297257523978331
7	42	0.7700520034701235
7	43	0.8574035597999874
7	44	1.3141962197822536
7	45	1.0948459775676547
7	46	0.6265076680722927
7	47	0.8240167611879308
7	48	1.089364635541905
7	49	1.265562587393024
7	50	0.9878964912610861
7	51	0.9416442234870246
7	52	0.100372672958148
7	53	0.818372542284969
7	54	0.2533175196165445
7	55	-0.34055975692662344
7	56	-0.3510164430739593
8	1	0.13207066010586033
8	2	0.5558595654755791
8	3	0.24085052019399456
8	4	0.16912994122707659
8	5	0.5526594762661645
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114	3	-0.3623240829806065
114	4	0.011061158158291562
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115	1	0.10120646257499999
115	2	0.6484521580681673
115	3	0.20998632266313422
115	4	0.48394475604188614
115	5	0.8983384886118415
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115	16	0.30170038392648024
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115	38	-0.17439448245690414
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115	49	-0.6066897462414218
115	50	-0.34280209350254154
115	51	-0.2877294591386921
115	52	1.7694877868379422E-4
115	53	-0.2965777738848594
115	54	-1.1772756752042923
115	55	-0.5825049541563736
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116	1	-0.4713620408878185
116	2	-0.701367173458479
116	3	-0.8960058483696898
116	4	-0.8076089928289321
116	5	-0.8491773198795798
116	6	-1.795535080390259
116	7	-4.317496552004172
116	8	-1.3306650429307751
116	9	-1.3787715475706719
116	10	-1.6803160253425347
116	11	-1.6184932566002246
116	12	-1.5361345201306662
116	13	-1.9348474258036674
116	14	-1.5411947645004886
116	15	-1.924699008276157
116	16	-1.0240298178199794
116	17	-1.1758223406265174
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116	19	-1.3307051342108736
116	20	-1.278544516044711
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116	24	-1.126751367397901
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116	34	0.15117295021568822
116	35	-0.28984726638166336
116	36	0.13517763327665477
116	37	0.3381824004649019
116	38	0.3184540571396042
116	39	0.06145066057204218
116	40	0.21126803438453834
116	41	0.18094526459295324
116	42	-0.034826045310367704
116	43	0.2171596573609591
116	44	0.1251718295383526
116	45	0.216797197079849
116	46	0.14480035099912314
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116	48	0.5466817087126365
116	49	-0.24053497358258724
116	50	-0.04868887459257465
116	51	-0.19859967895200015
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116	54	-1.0515605291639432
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117	1	0.3334463214815244
117	2	0.8946955443115527
117	3	-0.001371702028230004
117	4	0.7708230629201935
117	5	0.9934707637441136
117	6	0.03662519835538802
117	7	-1.6981237297045197
117	8	0.4204499396476251
117	9	0.5571866405826356
117	10	0.07334251124282787
117	11	-0.5740333262866315
117	12	-0.3854028128135951
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117	14	-0.6163167157200036
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117	30	-1.1424733120980832
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117	32	-0.44505389876736245
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117	35	-0.45204238833288457
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117	38	0.08775719302810359
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117	41	-0.0326784287868378
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117	53	0.6093481520410684
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117	55	1.3827155043974173
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118	1	0.6863034643386641
118	2	1.135886020502035
118	3	0.7150568694003425
118	4	0.832489729586861
118	5	1.2984707637441133
118	6	0.940672817403005
118	7	0.780209603628812
118	8	0.649735653933341
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118	10	0.9828663207666395
118	11	0.5114428641895614
118	12	0.5036448062340284
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118	14	0.4065404271371449
118	15	0.4963230590873735
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118	20	0.535044334129509
118	21	0.31156235744339966
118	22	0.07874017202442474
118	23	0.5828221520208388
118	24	0.32446814479722264
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118	26	0.8499649938131668
118	27	1.0071799648833064
118	28	0.8218756468694082
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118	30	0.5208600212352508
118	31	0.4366674565062141
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118	33	0.30661810471857365
118	34	0.161045191795246
118	35	-0.040137626428126794
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118	37	-0.3798779944247599
118	38	-0.34414756887665376
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118	40	-0.22441721532510073
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118	42	0.29357116723318555
118	43	0.2737798664201918
118	44	0.6543530142073415
118	45	0.1363674642110908
118	46	0.37520104786323216
118	47	-0.1656754571628234
118	48	0.2946491883874316
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118	53	-2.1087470860541693
118	54	-1.576967033228982
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118	56	-0.5760454790786049
119	1	0.17281140084660507
119	2	0.7632669728829811
119	3	0.16307274241621883
119	4	0.49320401530114566
119	5	0.6353755256488753
119	6	0.3807521824823681
119	7	0.6417175401367459
119	8	0.30727533647301897
119	9	0.5725834659794629
119	10	0.8455647334650536
119	11	0.37215714990384186
119	12	0.27348607607529374
119	13	0.10309295360013238
119	14	0.41923883983555754
119	15	0.10267226543657415
119	16	-0.013731714838947084
119	17	-0.24099849238803728
119	18	-0.024796997061741877
119	19	-0.48138264098594163
119	20	-0.9967016976165226
119	21	-0.10629478541374482
119	22	-0.4756249073406593
119	23	-0.5056699114712231
119	24	-0.5255318552027788
119	25	-0.6502033657803636
119	26	-0.5547969109487383
119	27	-0.3963914636881185
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119	30	-0.2092987089234839
119	31	-0.5351579403191806
119	32	-0.08902215273561609
119	33	-0.10052475242428471
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119	35	-0.7798201661106603
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119	37	0.4685347039879417
119	38	0.022519097790009823
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119	40	0.04026532435743846
119	41	0.41319458708617773
119	42	0.41341243707445585
119	43	0.7396528822932105
119	44	0.595226030080358
119	45	0.8220817499253776
119	46	0.49385184151402584
119	47	-0.2319452984326631
119	48	0.1890936328318773
119	49	-0.06533172155006639
119	50	0.3300374126703005
119	51	0.00424584950328466
119	52	0.12178188705028958
119	53	0.27934815204106656
119	54	-0.10196703322898415
119	55	-0.08744322576131225
119	56	-0.3966803997135262
120	1	-0.28653656987861353
120	2	-0.11457733849426432
120	3	-0.6071202050222411
120	4	-0.8546003759164194
120	5	-0.2272991250497256
120	6	-0.524503971875582
120	7	-0.3979697519457517
120	8	-0.6598237985901889
120	9	-0.37475519004182445
120	10	-0.2248610815715395
120	11	-0.41417019540553923
120	12	-0.48815730383155653
120	13	-0.4047247443373294
120	14	-0.11955024865413577
120	15	-0.13099373589409424
120	16	-0.46206837485225805
120	17	-0.4229013533328114
120	18	-0.6566000576073208
120	19	-0.1455875644723008
120	20	-0.9386511320809277
120	21	-0.6020033682480772
120	22	-0.465278932623427
120	23	-0.7026426326954365
120	24	-0.5857114959213376
120	25	-0.9902898594596721
120	26	0.1378178595103492
120	27	0.026462827729041294
120	28	-0.24003624363529497
120	29	-0.3476180225418801
120	30	-0.7134570588902136
120	31	-0.26296898489669474
120	32	-0.3716236164747997
120	33	-1.1138980059172994
120	34	-0.9910791309877567
120	35	-0.9562340050993541
120	36	-1.4362770183956108
120	37	-0.9311326280147227
120	38	-1.3566226187768535
120	39	-1.7707021070634177
120	40	-1.568051375576026
120	41	-1.8087947675379077
120	42	-1.5220832382415779
120	43	-1.3988035381991395
120	44	-1.4800567377173799
120	45	-1.6178384097552616
120	46	-1.6619033148399325
120	47	-1.5424576071485667
120	48	-1.6808997138081772
120	49	-1.2466690468993669
120	50	-1.33330257402298
120	51	-1.2395665257462163
120	52	-1.4214982194034675
120	53	-1.2419093329888717
120	54	-0.9494720232489442
120	55	-1.2819209370054914
120	56	-1.420499428322973
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	418.0
5	62.0
6	97.0
7	162.0
8	498.0
9	541.0
10	460.0
11	657.0
12	747.0
13	647.0
14	949.0
15	1057.0
16	994.0
17	1157.0
18	1277.0
19	1461.0
20	1639.0
21	1939.0
22	2244.0
23	1871.0
24	2447.0
25	2255.0
26	2249.0
27	2409.0
28	2952.0
29	3283.0
30	3819.0
31	5055.0
32	6113.0
33	7227.0
34	9054.0
35	11093.0
36	14350.0
37	22775.0
38	28348.0
39	26804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.23210260718321	0.34076186306815825	0.2810102169398875	0.14612531280874147
8	98.67305304239844	0.9071018863461653	0.2501330494944119	0.169712021760984
9	95.13511915321389	2.799361362426823	1.001714860150198	1.0638046242090946
10	33.52906392289043	44.46868901898173	8.7741706581515	13.228076399976347
11	22.190290343563362	42.3132872095086	23.147064041156643	12.349358405771392
12	53.48707941576488	19.88646443143516	15.170007687304121	11.456448465495832
13	25.105552598900122	18.238424693986165	24.703447460232987	31.952575246880727
14	28.677192360002362	37.7724557980013	18.49565371651588	15.054698125480456
15	43.23517237301165	23.467565489917806	17.862929454201407	15.43433268286914
16	21.393176039264382	25.525989001241793	31.699485541954942	21.381349417538882
17	34.957719827331324	25.358642303825913	16.57855833481166	23.105079534031102
18	33.15238602093312	29.376145703979656	18.927916740583054	18.543551534504168
19	20.510318727455502	28.156821004080186	33.49417538880019	17.838684879664125
20	22.662172550410975	37.104251670510315	17.0451185618828	23.188457217195907
21	23.72715983679262	29.981077405239194	28.30938442433919	17.982378333628997
22	24.19076340843238	31.584767311217547	16.14688664183076	28.077582638519306
23	27.138118018556078	40.64242589099338	16.127468082715883	16.091988007734656
24	19.366798928501737	47.34579472411285	18.391096866518044	14.89630948086737
25	21.054904489496508	28.439590863646853	23.853075325812387	26.65242932104426
26	30.23832345249506	28.811222738944704	21.13493556746778	19.81551824109245
27	19.415487163094117	28.0245313064223	22.310820650340087	30.249160880143496
28	20.009945183097923	39.788428069095346	23.300735233179026	16.900891514627705
29	27.80123532094064	31.750590715550477	24.357023148943227	16.091150814565655
30	16.357763091604184	44.94324748836796	26.804374240583233	11.894615179444626
31	24.48316737790422	36.41891891891892	28.51884779516358	10.579065908013277
32	13.905234185925117	36.8955535519385	38.76939685853936	10.42981540359702
33	18.602153727491174	37.18235486071969	30.20617639206147	14.00931501972767
34	15.997649935313877	38.980214354385005	31.68433171519115	13.337803995109967
35	12.379719935177876	47.11357524887066	31.7954897572732	8.711215058678269
36	15.600061751852557	39.45884001520046	32.315813224396734	12.625285008550257
37	12.175529045021282	39.39931658773455	38.058869372339785	10.366284994904381
38	14.820514953620897	38.68272653264958	36.841229566157814	9.655528947571709
39	12.178005431197894	38.71956835643871	36.178670960917955	12.92375525144545
40	12.911312508187544	39.067989377032	38.52314544653384	9.497552668246614
41	14.032490159001988	39.94077562524529	36.480668831091606	9.546065384661125
42	14.64906019509874	39.128003806804664	36.02486319295741	10.198072805139187
43	12.658996351342502	40.94888903438547	36.61276018261144	9.779354431660584
44	11.781027460880749	39.425298306618714	37.08409745873723	11.709576773763308
45	11.785405936467296	42.34898329810355	36.10110452826819	9.764506237160974
46	11.504166492938703	40.019179567208894	37.702144895078234	10.774509044774163
47	14.089271357532084	39.79275228538249	36.3296906172766	9.788285739808831
48	15.75733360367828	39.80511309761639	34.61687548826087	9.820677810444458
49	15.628968502387808	40.79068963255767	34.49593684976182	9.084405015292708
50	11.993370139394012	42.47999713818967	35.95208852533298	9.57454419708334
51	11.47846818655938	43.53568767694829	35.61853673074057	9.36730740575175
52	14.061735904867549	41.48447656874322	34.44078672725755	10.01300079913169
53	12.756337608112139	41.48165821652252	34.150909633164325	11.611094542201014
54	12.98288318925143	42.59991766748403	33.41447262443844	11.002726518826107
55	14.62042384611713	41.826165815474205	32.19953701493962	11.353873323469047
56	14.49103296860644	41.6594079253523	32.49370577636713	11.355853329674133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	32.0
5	64.0
6	89.5
7	115.0
8	193.5
9	272.0
10	1021.0
11	1770.0
12	1571.0
13	1239.0
14	1106.0
15	1019.5
16	933.0
17	1347.5
18	1762.0
19	2238.5
20	2715.0
21	3182.5
22	4375.5
23	5101.0
24	5818.0
25	6535.0
26	7425.0
27	8315.0
28	9132.5
29	11018.0
30	12086.0
31	13380.0
32	14674.0
33	11959.5
34	9245.0
35	10225.0
36	11205.0
37	8378.0
38	4915.0
39	4279.0
40	4028.0
41	3777.0
42	3759.5
43	3742.0
44	3717.5
45	3693.0
46	3719.5
47	3681.5
48	3617.0
49	3689.0
50	3761.0
51	4244.5
52	4728.0
53	5489.0
54	6791.0
55	7332.0
56	6959.5
57	6587.0
58	6119.0
59	5651.0
60	5158.0
61	4665.0
62	4255.0
63	3253.5
64	2662.0
65	2143.5
66	1625.0
67	1328.5
68	1032.0
69	852.0
70	672.0
71	556.0
72	339.0
73	238.0
74	190.0
75	142.0
76	120.5
77	99.0
78	68.0
79	31.5
80	26.0
81	23.0
82	20.0
83	15.5
84	11.0
85	9.0
86	7.0
87	5.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.04553249364319082
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	5.913310862752055E-4
24	5.913310862752055E-4
25	0.04198450712553959
26	0.05676778428241973
27	0.10821358878836261
28	0.1088049198746378
29	0.14605877830997574
30	0.23357577907870616
31	0.23061912364733014
32	0.3110401513807581
33	0.3341020637454911
34	0.3571639761102241
35	0.3837738749926084
36	0.4103837738749926
37	1.3600614984329726
38	0.48666548400449416
39	0.48666548400449416
40	0.6930400331145409
41	0.5534858967535923
42	0.5854177754124534
43	0.6522381881615517
44	0.6871267222517888
45	0.6889007155106144
46	0.7231979185145763
47	0.7066406480988705
48	0.8414641357696173
49	0.8178108923186092
50	0.8184022234048843
51	0.7894270001773993
52	0.8444207912009936
53	0.8633433859617999
54	0.8852226361539826
55	0.8881792915853587
56	0.8846313050677074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	169110.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.24457453728343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.551524533156	43.15238602093312
2	10.742044847546685	11.653953048311749
3	4.143547033237766	6.742948376796168
4	1.834672364361789	3.9808408728046834
5	0.9843785769570383	2.669859854532553
6	0.628999378631463	2.0471882206847614
7	0.3913531662542378	1.4860150198095914
8	0.2801609017474627	1.2157767133818225
9	0.22565488973433767	1.1016498137307078
>10	1.0835795188209258	10.013009283898054
>50	0.06649733465601255	2.4421973863165984
>100	0.061046733454700054	6.353852522027083
>500	0.0032703607207875025	1.1791141860327596
>1k	0.0032703607207875025	5.961208680740347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	4842	2.863225119744545	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	4007	2.3694636627047485	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1232	0.7285198982910531	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805	0.47602152445154045	No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.3749039086984803	No Hit
GGACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	555	0.3281887528827391	No Hit
GGACATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	461	0.27260363077286975	No Hit
GGACATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	459	0.2714209686003193	No Hit
GGACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.2513157116669623	No Hit
GGACATGGGGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.22293181952575247	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	370	0.21879250192182606	Illumina Single End Adapter 2 (95% over 23bp)
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	364	0.2152445154041748	No Hit
GGACATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	344	0.2034178936786707	No Hit
GGACATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	308	0.18212997457276328	No Hit
GGACATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.17858198805511205	No Hit
GGACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.17680799479628642	No Hit
GGACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.1649813730707823	No Hit
GGACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	271	0.1602507243805807	No Hit
GGACATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.14724144048252616	No Hit
GGACATGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	241	0.1425107917923245	Illumina Single End Adapter 2 (96% over 30bp)
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.13009283898054522	No Hit
GGACATGGGGAGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	209	0.12358819703151794	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAGCAGT	207	0.12240553485896753	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	190	0.11235290639228905	No Hit
GGACATGGGGTGCTGGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186	0.10998758204718823	No Hit
GGACATGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.10821358878836261	No Hit
GGACATGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.10584826444326179	No Hit
GGACATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.10407427118443616	No Hit
GGACATGGGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	175	0.10348294009816096	No Hit
GGACATGGGAGTGCAGTGCTGGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	173	0.10230027792561054	No Hit
GGACATGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	170	0.10052628466678494	Illumina Single End Adapter 2 (97% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0017739932588256165	0.0	0.0	0.0	0.0
8	0.008278635207852877	0.0	0.0	0.0	0.0
9	0.012417952811779315	0.0	0.0	0.0	0.0
10	0.08219502099225356	0.0	0.0	0.0	0.0
11	0.14132812961977412	0.0	0.0	0.0	0.0
12	0.15374608243155344	0.0	0.0	0.0	0.0
13	0.15965939329430548	0.0	0.0	0.0	0.0
14	0.21347052214534917	0.0	0.0	0.0	0.0
15	0.22470581278457807	0.0	0.0	0.0	0.0
16	0.2371237655963574	0.0	0.0	0.0	0.0
17	0.24776772514931109	0.0	0.0	0.0	0.0
18	0.26136834013364085	0.0	0.0	0.0	0.0
19	0.27260363077286975	0.0	0.0	0.0	0.0
20	0.33055407722783986	0.0	0.0	0.0	0.0
21	0.3488853409023712	0.0	0.0	0.0	0.0
22	0.4021051386671397	0.0	0.0	0.0	0.0
23	0.44763763231033055	0.0	0.0	0.0	0.0
24	0.4913961326946958	0.0	0.0	0.0	0.0
25	0.5174147004908048	0.0	0.0	0.0	0.0
26	0.544024599373189	0.0	0.0	0.0	0.0
27	0.5647211873928213	0.0	0.0	0.0	0.0
28	0.6699781207498078	0.0	0.0	0.0	0.0
29	0.6930400331145409	0.0	0.0	0.0	0.0
30	0.7468511619655845	0.0	0.0	0.0	0.0
31	0.7746437230205192	0.0	0.0	0.0	0.0
32	0.7858790136597481	0.0	0.0	0.0	0.0
33	0.7982969664715274	0.0	0.0	0.0	0.0
34	0.8148542368872331	0.0	0.0	0.0	0.0
35	0.9892969073384188	0.0	0.0	0.0	0.0
36	1.0105848264443262	0.0	0.0	0.0	0.0
37	1.0312814144639584	0.0	0.0	0.0	0.0
38	1.0549346579149665	0.0	0.0	0.0	0.0
39	1.0709005972443972	0.0	0.0	0.0	0.0
40	1.0981018272130565	0.0	0.0	0.0	0.0
41	1.130625036958193	0.0	0.0	0.0	0.0
42	1.1548696114954764	0.0	0.0	0.0	0.0
43	1.185618827981787	0.0	0.0	0.0	0.0
44	1.2140027201229968	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGC	20	3.8938224E-4	50.707783	37
AGCCACA	15	0.008010999	50.631435	35
TGCATTA	15	0.008010999	50.631435	35
TGCGATT	15	0.008010999	50.631435	36
TTGCGAT	15	0.008010999	50.631435	35
ATAACGG	15	0.008010999	50.631435	36
CATATGC	20	3.9933805E-4	50.449142	33
GCGGTCG	15	0.008126079	50.449142	33
TGGAGGG	15	0.008135724	50.43401	32
AGCCATA	15	0.008135724	50.43401	32
CCGGCCG	15	0.008261888	50.238125	39
ACCAGCT	15	0.008261888	50.238125	38
GCACGGG	15	0.008271653	50.223114	43
GTTATGG	15	0.008300999	50.178154	42
ACCGCGA	25	2.0983987E-5	49.92488	28
CCGCGAT	25	2.0983987E-5	49.92488	29
CACGTAT	20	4.2047224E-4	49.92488	28
ATCTAAA	15	0.008468758	49.924877	28
ACACGTA	15	0.008478704	49.91006	27
CACATTC	15	0.008478704	49.91006	27
>>END_MODULE