FastQCFastQC Report
Fri 17 Jun 2016
SRR1526586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238480
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC16140.6767863133176786No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT12270.5145085541764509No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC11370.476769540422677No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.44993290841999334No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10380.43525662529352566No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10140.4251928882925192No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9120.38242200603824217No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG6220.26081851727608185No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG5940.24907749077490776No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.2469808788996981No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5730.24027172089902715No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT5500.23062730627306272No Hit
CGCACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG4800.20127474002012746No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA3880.16269708151626971No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCCA3810.15976182489097618No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC3720.1559879235155988No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3540.148440120764844No Hit
CGCACCGGGCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTCCTGAGGATGATGAAACTGC3520.14760147601476015No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCTG3330.13963435088896345No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.13208654813820866No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC3090.12957061388795707No Hit
CGCACCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2990.12537739013753774No Hit
CGCACCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTT2880.12076484401207649No Hit
CGCACCGGGCGCTGAGCTTAGAATAGCCCATGTGTTACCTGTCTGTCTGTCCTCGTGCCTGCAC2850.11950687688695068No Hit
CGCACCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA2820.1182489097618249No Hit
CGCACCGGCAGGAGCTCAGGGACTATGCATCGTGGAACCTGAGTGTGAACCTGTACACTGTCAA2550.10692720563569272No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCCA2510.10524991613552498No Hit
CGCACCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2420.10147601476014759No Hit
CGCACCGGGGCTCATCGCCATGGCATTTCTGGCCAGCACCTTTTCCATCCTGGCTGTCAGTGAG2410.10105669238510567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC150.0046237858.18565452
TCGTCTA150.0046237858.18565454
GTGCGAC201.9756466E-458.1856555
ACGCACC201.979772E-458.1611150
ACTCGAA150.00463151158.1611150
TACTCGA150.00463151158.1611149
ATGCGCT150.004635379658.14885346
CGCGCAT150.00464700158.11209539
CATATAG150.004670309458.0387232
TCTCGTG150.004674202358.02651229
ACGTATG150.004674202358.02651229
GTTAACT258.711588E-657.92900522
TTGCGCA150.004732889657.8439619
ACCGGTA303.8249527E-757.831834
CGGATAC150.004736821657.831836
GCTTATA150.004736821657.8318311
CGAGGGT150.004736821657.8318316
GTGTACG150.004736821657.831839
GACTATA150.004736821657.831839
CGCACCG240100.057.0249371
Produced by FastQC (version 0.11.5)