FastQCFastQC Report
Fri 17 Jun 2016
SRR1526585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231298
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC12330.533078539373449No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT12180.5265933989917769No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9230.39905230481889165No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC8950.38694670943977033No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.3683559736789769No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8450.3653295748341966No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7870.34025369869173105No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG6010.2598379579589966No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG4920.2127126045188458No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4590.19844529567916713No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.19498655414227534No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT4440.191960155297495No Hit
CGCACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG3390.1465641726257901No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCCA2870.1240823526359934No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA2820.12192063917543601No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2780.12019126840699011No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCTG2670.11543549879376389No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2610.11284144264109502No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC2600.11240909994898356No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1119767572568721No Hit
CGCACCGGCAGGAGCTCAGGGACTATGCATCGTGGAACCTGAGTGTGAACCTGTACACTGTCAA2510.10851801571998029No Hit
CGCACCGGGCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTCCTGAGGATGATGAAACTGC2500.10808567302786881No Hit
CGCACCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2460.1063563022594229No Hit
CGCACCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA2370.10246521803041964No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCCA2340.10116818995408522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC201.9645371E-458.2512538
ACCCTAA150.004622918658.187858
TGCGATC258.516432E-658.14979632
TACCCGC406.9849193E-1058.1497932
ATCGAAC150.004634895458.1497952
GATGCTA258.538445E-658.1244848
ACTCGTC150.004666947858.04868730
ACTCACG201.9986715E-458.04868330
ACGTGGC150.004711288557.91023326
CAATAGT150.004731546657.8475213
GATAAAT150.004751869557.7849439
GCACTTC303.8459257E-757.78494310
CGCTAAA150.00475594257.7724419
ACTAGAT453.274181E-1157.7599518
CGCACCG231800.056.9502561
ACCGGGG112750.056.9393124
CACCGGG221600.056.8592383
GCACCGG230900.056.7213362
ACCGGGA72650.056.1543964
CCGGGGG55450.056.0654645
Produced by FastQC (version 0.11.5)