FastQCFastQC Report
Fri 17 Jun 2016
SRR1526584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211427
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20160.9535206004909496No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC14520.6867618610678863No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12080.5713555979132278No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11020.5212200901493187No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9850.4658818410136832No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC7620.3604080841141387No Hit
CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6720.31784020016364983No Hit
CGCACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5810.2747993397248223No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC5740.27148850430645094No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5520.2610830215629981No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA5240.2478396798895127No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC5050.23885312661107616No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC4930.23317740875101098No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3400.16081200603518No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3390.16033902954684123No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC3280.15513628817511482No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCA3120.14756866436169458No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.136690205129903No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2880.13621722864156424No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCA2790.13196044024651535No Hit
CGCACCGGGCGCTGAGCTTAGAATAGCCCATGTGTTACCTGTCTGTCTGTCCTCGT2590.12250091047974006No Hit
CGCACCGGGGCCTCTTCGAGATCATTGGGGTCAAGAGCCAAGAAGCCAGCCAGACC2360.11162245124794846No Hit
CGCACCGGGCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTCCTGAGGATGAT2340.11067649827127092No Hit
CGCACCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2320.1097305452945934No Hit
CGCACCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA2300.10878459231791589No Hit
CGCACCGGCAGGAGCTCAGGGACTATGCATCGTGGAACCTGAGTGTGAACCTGTAC2230.10547375689954452No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCA2170.10263589796951193No Hit
CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2160.10216292148117317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTTG150.0080006250.65254237
ATATTCA150.00803877550.59163336
GACTACG150.008100104550.4944833
GTTAAGC203.9921925E-450.45814532
AGTCTTA150.00812319350.45814532
GCGCGAT150.00829400550.1932738
CGCTCTA150.00829400550.1932738
TTGAACA204.0972707E-450.19326838
CCAATCA150.00830966550.1693342
CATAAAT150.00830966550.1693342
AACTCGC204.1069312E-450.16932742
ACGTACT301.0247386E-650.1334641
CAACTCG204.1214557E-450.1334641
TACAGCG150.00849133849.89563429
ATAGGTC150.00851525149.86015725
TTACGGA204.2440885E-449.83653324
CTATGCA150.00853921349.8247323
AATCTCC150.00853921349.8247323
CACTATA252.1253203E-549.82472623
AATTACG204.2589972E-449.80113222
Produced by FastQC (version 0.11.5)