##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526584_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 211427 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.69378556191972 39.0 39.0 40.0 36.0 40.0 2 38.11855155680211 39.0 38.0 40.0 35.0 40.0 3 38.250091047974 39.0 38.0 40.0 35.0 40.0 4 38.15105923084563 39.0 38.0 40.0 35.0 40.0 5 38.30416644988578 39.0 39.0 40.0 35.0 40.0 6 38.602165286363615 39.0 39.0 40.0 36.0 40.0 7 38.41805918827775 39.0 39.0 40.0 36.0 40.0 8 38.652286604832874 39.0 39.0 40.0 37.0 40.0 9 38.58551178420921 39.0 39.0 40.0 36.0 40.0 10 37.794638338528195 39.0 38.0 40.0 34.0 40.0 11 37.90628443860056 39.0 38.0 40.0 35.0 40.0 12 37.7805436391757 39.0 38.0 40.0 34.0 40.0 13 37.720726302695496 39.0 38.0 40.0 34.0 40.0 14 37.697943025252215 39.0 38.0 40.0 33.0 40.0 15 37.77135843577216 39.0 38.0 40.0 33.0 40.0 16 37.62711952588837 39.0 38.0 40.0 33.0 40.0 17 37.50886594427391 39.0 38.0 40.0 33.0 40.0 18 37.442024906941874 39.0 37.0 40.0 33.0 40.0 19 37.30515970051129 39.0 37.0 40.0 33.0 40.0 20 37.22488140114555 39.0 37.0 40.0 33.0 40.0 21 37.135994929692046 39.0 36.0 40.0 33.0 40.0 22 37.102262246543724 39.0 36.0 40.0 33.0 40.0 23 36.929190689930806 39.0 36.0 40.0 32.0 40.0 24 36.79548496644232 39.0 36.0 40.0 32.0 40.0 25 36.70771944926618 39.0 36.0 40.0 31.0 40.0 26 36.298093431775506 38.0 36.0 40.0 31.0 40.0 27 36.114001522984296 38.0 35.0 40.0 30.0 40.0 28 35.917314250308614 38.0 35.0 40.0 30.0 40.0 29 35.74238862586141 38.0 35.0 40.0 29.0 40.0 30 35.41997947282041 38.0 35.0 40.0 29.0 40.0 31 34.73052164576899 38.0 34.0 39.0 27.0 40.0 32 34.6833422410572 38.0 34.0 39.0 27.0 40.0 33 34.40997602009204 38.0 33.0 39.0 26.0 40.0 34 34.37815889172149 38.0 33.0 39.0 26.0 40.0 35 34.27567907599313 38.0 33.0 39.0 26.0 40.0 36 33.18071958642936 38.0 33.0 39.0 17.0 40.0 37 32.65685082794534 37.0 32.0 39.0 9.0 40.0 38 32.63129117851552 37.0 32.0 39.0 12.0 40.0 39 32.48628131695573 37.0 32.0 39.0 10.0 40.0 40 32.19708457292588 37.0 31.0 39.0 4.0 40.0 41 31.668117127897574 36.0 31.0 39.0 4.0 40.0 42 31.546812847933328 36.0 31.0 39.0 4.0 40.0 43 31.410974000482437 36.0 30.0 39.0 4.0 40.0 44 30.960577409696963 36.0 30.0 39.0 4.0 40.0 45 30.76996315513156 36.0 30.0 39.0 4.0 40.0 46 30.092911501369265 35.0 29.0 39.0 4.0 40.0 47 29.860188150047062 35.0 28.0 39.0 4.0 40.0 48 29.46825145322026 35.0 28.0 38.0 4.0 40.0 49 29.38836572433984 35.0 28.0 38.0 4.0 40.0 50 28.95241856527312 35.0 27.0 38.0 4.0 40.0 51 28.270055385546783 34.0 25.0 38.0 4.0 40.0 52 27.963022698141675 34.0 23.0 38.0 4.0 40.0 53 27.64566493399613 34.0 22.0 38.0 4.0 40.0 54 27.36751692073387 34.0 18.0 38.0 4.0 40.0 55 26.901578322541585 33.0 4.0 38.0 4.0 40.0 56 15.977320777384156 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.7920326163195455 1 2 -0.30980353576244823 1 3 -0.8761498438659032 1 4 -0.4437636720876057 1 5 -0.3786215468870182 1 6 -0.7325393489181877 1 7 -1.6247376092390624 1 8 -1.5535298433394118 1 9 -1.499081941873328 1 10 -0.7817552766155629 1 11 -0.7597094216084272 1 12 -0.8201608020008422 1 13 -1.1796207310968967 1 14 -1.1789323647600654 1 15 -1.3254399270354327 1 16 -1.4670302768518297 1 17 -1.0372222996268192 1 18 -1.5384919710939329 1 19 -1.4105139686393926 1 20 -1.4610587029429496 1 21 -1.1316287887959078 1 22 -1.0661190655538704 1 23 -1.0910916870342149 1 24 -1.0179020213059076 1 25 -1.0758445567370316 1 26 -1.0959933865807514 1 27 -0.7703807750231206 1 28 -0.23768741235909374 1 29 -0.19962907914850803 1 30 -0.9122257397090365 1 31 -0.23121848360861463 1 32 0.07718125650065844 1 33 -0.3264200795684289 1 34 -0.5511946765087856 1 35 0.2627660949618331 1 36 0.4268037148310668 1 37 0.2234240065478872 1 38 0.3476245184553477 1 39 0.417633058948617 1 40 0.8533955688345216 1 41 1.012490985752386 1 42 0.8815751333602968 1 43 0.986819928035171 1 44 1.046264638922338 1 45 1.3314638447323723 1 46 0.7967115962669453 1 47 1.1201830696347166 1 48 1.2538806438776007 1 49 1.563827690977032 1 50 1.6418221846910868 1 51 2.322723695632213 1 52 1.9211724240530081 1 53 2.059901183953926 1 54 2.4880841467671537 1 55 1.72913896411033 1 56 2.5763297444110744 2 1 -0.08143052207870483 2 2 0.48912316580823756 2 3 0.15343130796655657 2 4 -0.10666943124991235 2 5 0.6299386625370644 2 6 0.1846596039613928 2 7 0.561937783431091 2 8 0.3687738215820531 2 9 0.2993997335193441 2 10 0.06073163437920215 2 11 -0.1949450237026653 2 12 -0.44832834126786025 2 13 -0.2570552860707167 2 14 -0.8001889092626797 2 15 -0.2548116547841204 2 16 -0.45273708313455074 2 17 -0.6237144462236799 2 18 -0.5990678873243027 2 19 -0.5099118743985542 2 20 -0.6002733626288119 2 21 -0.7105031343456503 2 22 -0.6173494320460122 2 23 -0.9290759802279283 2 24 -0.8428234872744937 2 25 -0.35916916406687704 2 26 -0.8895274179943655 2 27 -0.7445692566985187 2 28 -1.188132438537103 2 29 -2.008712848782011 2 30 -0.7482466821174114 2 31 -1.4608258134515495 2 32 -1.340384188525519 2 33 -1.441943639777854 2 34 -1.5325821110637605 2 35 -1.3199040621062252 2 36 -1.2879868610851588 2 37 -1.1690890824573472 2 38 -1.3213021831153355 2 39 -1.7815292447163067 2 40 -1.1323635934691438 2 41 -1.086776029954418 2 42 -1.1916185839171938 2 43 -1.4309025850538362 2 44 -1.1144945233813246 2 45 -1.3628293489849064 2 46 -1.4052779325288647 2 47 -1.9762828989516699 2 48 -1.57509841371402 2 49 -1.591460267138153 2 50 -1.4874448310157184 2 51 -1.9796585033206675 2 52 -1.4639322879888788 2 53 -1.5574286589780115 2 54 -1.4741933401438416 2 55 -1.9846568474079938 2 56 -0.6427016692014931 3 1 -0.031678046831181916 3 2 0.26095484897655297 3 3 -0.30349938510274654 3 4 0.10387512320553327 3 5 0.3924634150123154 3 6 0.09025366336733498 3 7 0.3024823378865378 3 8 0.16322926712661 3 9 0.04088488203419871 3 10 0.16404846606237555 3 11 -0.13623215241553766 3 12 -0.2128827967134015 3 13 -0.017550335575670317 3 14 -0.693654255797334 3 15 -0.3131284864672921 3 16 -0.5916479742236618 3 17 -0.4878728620652666 3 18 -0.6409490754431104 3 19 -0.47689207241835874 3 20 -0.6795802933218766 3 21 -0.683621946226836 3 22 -0.16056725382819081 3 23 -0.23496706933683953 3 24 -0.0590611110368755 3 25 -0.826842431393608 3 26 -0.48145811106366665 3 27 -0.2517969794707895 3 28 -0.36748887418067255 3 29 -0.7920296804651841 3 30 -0.7236922266718722 3 31 -0.3330535362238223 3 32 -0.6938000301096778 3 33 -1.103181263540229 3 34 -0.5286712199746475 3 35 -0.6398050522052401 3 36 -0.5624918115802089 3 37 -0.018891062655363555 3 38 -0.47065861875890036 3 39 -0.18311340313214686 3 40 -0.1959279499047888 3 41 -0.26439979233065714 3 42 0.024024980439243393 3 43 0.03578058326299782 3 44 -0.4040489788268715 3 45 -0.0538194479948082 3 46 0.06383097836222262 3 47 -0.004253195981373636 3 48 0.11485208133548497 3 49 -0.06413353446488301 3 50 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4 34 -0.3948636644618162 4 35 -0.47456425628098486 4 36 -0.4692975406968074 4 37 0.039745868998963374 4 38 0.016027913972038732 4 39 -0.25415060393960687 4 40 0.7583160181813398 4 41 -0.007358554226264857 4 42 0.07867267821873014 4 43 -0.07260161418004785 4 44 0.8104083892400347 4 45 0.4830929811121827 4 46 -0.25828764126672965 4 47 -0.1313799863303089 4 48 -0.3803896758499441 4 49 -0.030489393351743388 4 50 0.5734289553920497 4 51 -0.31543520234979283 4 52 -0.4463109287655769 4 53 -0.188205358007135 4 54 -0.5156011071341311 4 55 -0.9452879153691605 4 56 -0.19556574687139516 5 1 -0.1801928983163279 5 2 0.08273702719437637 5 3 -0.15993502866710685 5 4 0.07912264795800894 5 5 0.3924634150123154 5 6 -0.043409702969299246 5 7 0.5995120408568368 5 8 0.16322926712661 5 9 0.24385517906389964 5 10 0.3620682680425702 5 11 0.23010448124782812 5 12 0.08909740130639676 5 13 -0.06705528607071898 5 14 0.29149425905414716 5 15 -0.13986115973461466 5 16 0.19053024359811843 5 17 -0.03242731751080896 5 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1.3420759973635974 6 43 1.3100087449954252 6 44 0.800603998786162 6 45 1.5688455278623827 6 46 1.275214678308572 6 47 1.410539761146854 6 48 1.890689763625879 6 49 1.964106235324902 6 50 0.9941807847478294 6 51 1.5199227774675101 6 52 1.7124962834396946 6 53 1.3836058237806093 6 54 1.5097229160137964 6 55 1.4269441378136811 6 56 0.5743180352320056 7 1 -0.11540488105306679 7 2 0.7723282940133629 7 3 0.09573900027424997 7 4 0.11730492772444734 7 5 0.4885284061268109 7 6 -0.006750652448857863 7 7 0.36463009112340217 7 8 0.20877382158205648 7 9 0.29965614377575633 7 10 0.28547522412279136 7 11 0.045311386553741784 7 12 0.17269729975777892 7 13 0.20217548316004752 7 14 0.03929827022449928 7 15 -0.22942703939950349 7 16 -0.2981216985191679 7 17 -0.29602213853137016 7 18 -0.2367601950166076 7 19 -0.6826041820908628 7 20 -0.4846323369877865 7 21 -0.4108877497302643 7 22 -0.643887893584477 7 23 -0.18305033920228908 7 24 -0.2520542565052679 7 25 -0.5941691640668765 7 26 -0.5335017769687198 7 27 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0.5374627088005433 8 52 0.5501586211020317 8 53 0.2505511390017894 8 54 1.2405036295531282 8 55 0.5286259808748319 8 56 0.21401550251568047 9 1 -0.18014186228489137 9 2 -0.08680466924331398 9 3 -0.21306353739426953 9 4 -0.1045560291880534 9 5 -0.1694943271536573 9 6 -0.3812682310901536 9 7 0.4084841751836734 9 8 0.21186660508720934 9 9 0.11723478506573315 9 10 -0.25169104603316583 9 11 0.02371477011176637 9 12 -0.8384314340513654 9 13 -0.6804573479263922 9 14 -0.39070437318020623 9 15 -0.4489353661243243 9 16 -0.3796442996293976 9 17 -0.43464228127523086 9 18 -0.20020190794285497 9 19 -0.4893448640892757 9 20 -0.3061496512886066 9 21 -0.7223072580569863 9 22 0.06404232053130698 9 23 -0.3137667018774124 9 24 -0.12096781717140459 9 25 -1.032313493963784 9 26 -0.040506799437658 9 27 -0.10343523607996019 9 28 -0.04024584059896341 9 29 -1.1082489312562416 9 30 -0.6445353419112294 9 31 -0.9218051948948442 9 32 -0.9065182091440747 9 33 -0.7630261140046528 9 34 -0.7149016986926213 9 35 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-0.5850984137140216 120 49 -0.4464602671381499 120 50 -0.2724448310157186 120 51 -0.3546585033206675 120 52 -0.3189322879888792 120 53 -0.10242865897800968 120 54 -0.22919334014384063 120 55 0.26534315259200625 120 56 0.6322983307985073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 218.0 5 16.0 6 36.0 7 53.0 8 138.0 9 149.0 10 142.0 11 243.0 12 287.0 13 313.0 14 504.0 15 647.0 16 743.0 17 950.0 18 1150.0 19 1360.0 20 1409.0 21 1743.0 22 1966.0 23 2273.0 24 3399.0 25 5655.0 26 4210.0 27 3442.0 28 3967.0 29 4244.0 30 5115.0 31 6137.0 32 7403.0 33 9247.0 34 12393.0 35 16608.0 36 23266.0 37 32969.0 38 37814.0 39 21218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.04791198391104 0.46374068378090616 0.20205844079025198 0.28628889151780434 8 99.31891385679218 0.5060848425224782 0.0993250625511406 0.07567623813420235 9 96.00382165002578 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26.254924867684824 20.802924886603886 29.118324528087708 23.823825717623574 23 28.020073122165094 24.25565325147687 26.11681573308991 21.607457893268126 24 28.194868156556694 25.75381340901029 22.905522052737375 23.14579638169564 25 28.336274727822435 21.263206703540554 22.994923137340255 27.405595431296753 26 23.05002129572666 20.23425299323269 26.900761913775966 29.81496379726468 27 21.65133444754298 20.72193209633968 27.772701232416875 29.85403222370047 28 23.015523583632774 25.55828563583254 24.202398367632927 27.22379241290176 29 28.535842658849493 23.619168493883755 25.094833750870198 22.750155096396554 30 27.42300874889809 21.462288742073383 29.912605806690113 21.20209670233841 31 28.82797059618092 20.89879759044139 29.54979549084567 20.723436322532027 32 33.13400135627943 19.69687628929535 28.371848191548388 18.79727416287683 33 30.210769500804087 20.350665800122393 29.443213677484238 19.995351021589286 34 23.268083006765412 24.114471149740485 35.20386378084999 17.41358206264411 35 22.419275233015053 29.958807114789575 30.84435923233167 16.777558419863702 36 25.58412213160668 29.028092927873615 27.434040007975014 17.953744932544694 37 26.58708861244753 27.289586168561076 27.395008721320274 18.72831649767112 38 28.188050185988402 24.34333981006855 28.483541333130642 18.98506867081241 39 25.66169868064101 22.946489231807146 27.74502211601047 23.646789971541374 40 25.366696659382722 22.288872702343717 29.193386368072517 23.151044270201044 41 20.445145894142367 23.67535344507934 30.236073740955895 25.6434269198224 42 21.158171538348146 21.319847644046277 32.04372863141177 25.47825218619381 43 22.07110531600512 19.95345937688863 32.61174746239394 25.363687844712306 44 24.308434927903953 20.104062037349987 30.42981524942042 25.157687785325635 45 22.836332476435302 22.25126154432067 28.79272588784157 26.119680091402458 46 20.975876931869596 23.63489152954064 29.904984164027336 25.48424737456243 47 20.66330174753583 24.194085995904956 32.10228084376934 23.040331412789865 48 23.385285802148342 24.424653027753013 31.342166365504447 20.847894804594194 49 26.230141139122846 22.44927142469005 29.105356232095446 22.21523120409165 50 21.59478731720338 23.104825640145666 30.551583442963643 24.748803599687317 51 20.60037362511676 25.712461160144112 30.860290893840904 22.826874320898224 52 21.61579755332437 25.108937135882986 31.07354329357247 22.20172201722017 53 23.62848273592958 21.614778292237485 32.22403952067826 22.532699451154674 54 21.78858609078856 21.99366635825138 30.923242748266354 25.29450480269371 55 23.738320431553795 23.1807536928661 27.6222091853039 25.458716690276205 56 21.650010732940586 24.906623416891264 26.69830896558304 26.74505688458511 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 4.0 10 6.0 11 8.0 12 12.5 13 110.5 14 204.0 15 199.5 16 195.0 17 235.0 18 275.0 19 315.0 20 355.0 21 503.5 22 895.0 23 1138.0 24 1399.0 25 1660.0 26 1938.5 27 2217.0 28 2635.0 29 3467.0 30 3881.0 31 4426.5 32 4972.0 33 5208.0 34 5444.0 35 5553.5 36 5663.0 37 4871.5 38 4518.0 39 4956.0 40 4258.5 41 3561.0 42 3631.0 43 3701.0 44 3897.5 45 4094.0 46 4202.5 47 4796.5 48 5282.0 49 5848.5 50 6415.0 51 6322.5 52 6230.0 53 6455.0 54 7418.0 55 8156.0 56 9058.5 57 9961.0 58 10995.0 59 12029.0 60 12349.0 61 12669.0 62 13627.0 63 16410.5 64 18236.0 65 17869.0 66 17502.0 67 14802.0 68 12102.0 69 10179.0 70 8256.0 71 7296.0 72 5332.0 73 4328.0 74 3624.0 75 2920.0 76 2483.5 77 2047.0 78 1741.0 79 1133.5 80 832.0 81 637.0 82 442.0 83 350.5 84 259.0 85 207.0 86 155.0 87 113.0 88 49.0 89 27.0 90 21.0 91 15.0 92 13.5 93 12.0 94 7.0 95 2.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.048243601810553996 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 9.459529766775293E-4 25 0.035946213113746114 26 0.05533824913563546 27 0.10216292148117317 28 0.09459529766775293 29 0.12675769887478894 30 0.20290691349733003 31 0.20527179593902387 32 0.26297492751635315 33 0.29702923467674425 34 0.30696174093185824 35 0.3362862832088617 36 0.36371891953251007 37 1.2973745075132315 38 0.43986813415505116 39 0.4474357579684714 40 0.6508156479541402 41 0.5056118660341394 42 0.5344634318228041 43 0.6091937169803289 44 0.64324802414072 45 0.6465588595590913 46 0.691491625951274 47 0.6702076839760296 48 0.7964924063624796 49 0.7728435819455415 50 0.7714246524805253 51 0.7525055929469746 52 0.7912896649907533 53 0.8106817010126426 54 0.8291277840578546 55 0.8352764784062584 56 0.8475738671030664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 211427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.05697947755017 #Duplication Level Percentage of deduplicated Percentage of total 1 70.00254308100318 27.34087888491063 2 11.144749748719377 8.705605244363303 3 5.49668793974109 6.440520841708959 4 3.372609806604745 5.268958080093839 5 2.314203712898265 4.519290346076897 6 1.5791322038824371 3.700568044762495 7 1.1407534785715152 3.1188069641058145 8 0.8973442968381027 2.803804622872197 9 0.6648340336897683 2.3369768288818364 >10 3.1231456701018443 20.68704564696089 >50 0.16590576068396767 4.362262151948427 >100 0.08355837581893263 5.544230396306999 >500 0.009687927631180595 2.438193797386332 >1k 0.004843963815590297 2.7328581496213826 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2016 0.9535206004909496 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 1452 0.6867618610678863 No Hit CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1208 0.5713555979132278 No Hit CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1102 0.5212200901493187 No Hit CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 985 0.4658818410136832 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 762 0.3604080841141387 No Hit CGCACCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 672 0.31784020016364983 No Hit CGCACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 581 0.2747993397248223 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 574 0.27148850430645094 No Hit CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 552 0.2610830215629981 No Hit CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 524 0.2478396798895127 No Hit CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 505 0.23885312661107616 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 493 0.23317740875101098 No Hit CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 340 0.16081200603518 No Hit CGCACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 339 0.16033902954684123 No Hit CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 328 0.15513628817511482 No Hit CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCA 312 0.14756866436169458 No Hit CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.136690205129903 No Hit CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 288 0.13621722864156424 No Hit CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCA 279 0.13196044024651535 No Hit CGCACCGGGCGCTGAGCTTAGAATAGCCCATGTGTTACCTGTCTGTCTGTCCTCGT 259 0.12250091047974006 No Hit CGCACCGGGGCCTCTTCGAGATCATTGGGGTCAAGAGCCAAGAAGCCAGCCAGACC 236 0.11162245124794846 No Hit CGCACCGGGCAAGCAAAAGCTCAGCGTGGGAACCCTGGGACCTTCCTGAGGATGAT 234 0.11067649827127092 No Hit CGCACCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 232 0.1097305452945934 No Hit CGCACCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA 230 0.10878459231791589 No Hit CGCACCGGCAGGAGCTCAGGGACTATGCATCGTGGAACCTGAGTGTGAACCTGTAC 223 0.10547375689954452 No Hit CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCA 217 0.10263589796951193 No Hit CGCACCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 216 0.10216292148117317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 9.459529766775293E-4 0.0 0.0 0.0 0.0 9 9.459529766775293E-4 0.0 0.0 0.0 0.0 10 0.004729764883387647 0.0 0.0 0.0 0.0 11 0.006148694348403941 0.0 0.0 0.0 0.0 12 0.008513576790097763 0.0 0.0 0.0 0.0 13 0.008986553278436529 0.0 0.0 0.0 0.0 14 0.012770365185146647 0.0 0.0 0.0 0.0 15 0.01418929465016294 0.0 0.0 0.0 0.0 16 0.016554177091856765 0.0 0.0 0.0 0.0 17 0.019865012510228118 0.0 0.0 0.0 0.0 18 0.02128394197524441 0.0 0.0 0.0 0.0 19 0.02601370685863206 0.0 0.0 0.0 0.0 20 0.033581330672052295 0.0 0.0 0.0 0.0 21 0.035000260137068584 0.0 0.0 0.0 0.0 22 0.04587871936886018 0.0 0.0 0.0 0.0 23 0.05060848425224782 0.0 0.0 0.0 0.0 24 0.056284202112313 0.0 0.0 0.0 0.0 25 0.05912206104234558 0.0 0.0 0.0 0.0 26 0.06243289646071694 0.0 0.0 0.0 0.0 27 0.06479777890241076 0.0 0.0 0.0 0.0 28 0.07473028515752482 0.0 0.0 0.0 0.0 29 0.07709516759921864 0.0 0.0 0.0 0.0 30 0.08939255629602652 0.0 0.0 0.0 0.0 31 0.09412232117941417 0.0 0.0 0.0 0.0 32 0.09885208606280182 0.0 0.0 0.0 0.0 33 0.1031088744578507 0.0 0.0 0.0 0.0 34 0.104527803922867 0.0 0.0 0.0 0.0 35 0.14520378192000075 0.0 0.0 0.0 0.0 36 0.1499335468033884 0.0 0.0 0.0 0.0 37 0.1579741471051474 0.0 0.0 0.0 0.0 38 0.15986605305850246 0.0 0.0 0.0 0.0 39 0.1645958179418901 0.0 0.0 0.0 0.0 40 0.1697985593136165 0.0 0.0 0.0 0.0 41 0.17121748877863283 0.0 0.0 0.0 0.0 42 0.17500130068534292 0.0 0.0 0.0 0.0 43 0.17973106556873059 0.0 0.0 0.0 0.0 44 0.18446083045211822 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGTTG 15 0.00800062 50.652542 37 ATATTCA 15 0.008038775 50.591633 36 GACTACG 15 0.0081001045 50.49448 33 GTTAAGC 20 3.9921925E-4 50.458145 32 AGTCTTA 15 0.008123193 50.458145 32 GCGCGAT 15 0.008294005 50.19327 38 CGCTCTA 15 0.008294005 50.19327 38 TTGAACA 20 4.0972707E-4 50.193268 38 CCAATCA 15 0.008309665 50.16933 42 CATAAAT 15 0.008309665 50.16933 42 AACTCGC 20 4.1069312E-4 50.169327 42 ACGTACT 30 1.0247386E-6 50.13346 41 CAACTCG 20 4.1214557E-4 50.13346 41 TACAGCG 15 0.008491338 49.895634 29 ATAGGTC 15 0.008515251 49.860157 25 TTACGGA 20 4.2440885E-4 49.836533 24 CTATGCA 15 0.008539213 49.82473 23 AATCTCC 15 0.008539213 49.82473 23 CACTATA 25 2.1253203E-5 49.824726 23 AATTACG 20 4.2589972E-4 49.801132 22 >>END_MODULE