FastQCFastQC Report
Fri 17 Jun 2016
SRR1526583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93047
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.7909980977355531No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.5180177759626856No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.3783034380474384No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3300.3546594731694735No Hit
CGCTTGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC2880.30952099476608597No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2490.26760669339151183No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2170.2332154717508356No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.22891656904575108No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2110.22676711769320881No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2040.2192440379593109No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1980.2127956839016841No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1930.20742205552032844No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCC1670.17947918793727902No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1620.17410555955592336No Hit
CGCTTGGGGGATTATTACCAAAATCAAGACTGGAGAGATGGCTCAGTGCTTAAGAGCACCGACC1570.1687319311745677No Hit
CGCTTGGGGCTAGAGCAGTTTTCATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGA1540.16550775414575428No Hit
CGCTTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1420.1526110460305007No Hit
CGCTTGGGGGTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1400.15046159467795847No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1350.1450879662966028No Hit
CGCTTGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC1340.14401324062033166No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.13971433791524712No Hit
CGCTTGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1300.13971433791524712No Hit
CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.13326598385762034No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1160.12466817844745129No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAAAGGTCC1140.12251872709490902No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC990.10639784195084205No Hit
CGCTTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCAACTGCTCTTCCAGAGGCCT970.1042483905982998No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG960.10317366492202866No Hit
CGCTTGGGGATGCATTTGCTGTGGATTACCTAGTAAAGACAGTAAGGATAGGTTGTTTTTAACA950.10209893924575753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT150.00459095458.26204358
TCTAAGA150.00459095458.26204358
ATAGACA201.9618231E-458.23052254
TATAGAC201.9618231E-458.23052253
AGGTCAG150.004600810458.2305257
TTCACTC150.004600810458.2305253
ATGTATT150.004600810458.2305253
CACTCTA150.004600810458.2305255
AGCCTTG150.004600810458.2305254
GTATAGA201.9670819E-458.19902852
CTGAATC150.004610682458.19902452
TATAGAA150.004610682458.19902452
CTTTGTA201.9723517E-458.16757248
CATATAG150.0046205758.1675750
ATAGCAC258.4610965E-658.1675750
TAGCACA258.4610965E-658.1675751
ATATAGA150.0046205758.1675751
AAGCAAC150.0046205758.1675751
TCATATA150.0046205758.1675749
TCATAGC258.4610965E-658.1675748
Produced by FastQC (version 0.11.5)