FastQCFastQC Report
Fri 17 Jun 2016
SRR1526582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90278
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.6978444360752343No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.3810452158886994No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2670.2957531181461707No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.2713839473625911No Hit
CGCTTGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCC2320.2569839828086577No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2270.2514455349032987No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.2281840537007909No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2050.22707636411971907No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1700.18830722878220607No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1560.17279957464720086No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1470.16283036841755466No Hit
CGCTTGGGGGATTATTACCAAAATCAAGACTGGAGAGATGGCTCAGTGCTTAAGAGCACCGACC1460.16172267883648286No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1410.15618423093112385No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCC1340.14843040386362125No Hit
CGCTTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1270.14067657679611864No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1220.13513812889075966No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1170.12959968098540064No Hit
CGCTTGGGGCTAGAGCAGTTTTCATATTGGAAAATGACAATGTCTTTTTTCTTTCACTCTAAGA1140.12627661224218523No Hit
CGCTTGGGGGTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1070.11852278517468266No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAAAGGTCC1060.11741509559361084No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.11187664768825185No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG980.10855357894503644No Hit
CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.10633819978289284No Hit
CGCTTGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC930.10301513103967745No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG920.10190744145860564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCC406.8030204E-1058.25335734
GTGGAAA150.00459318758.25335333
TACTGTC150.00459318758.25335334
CACAGTC150.00459318758.25335333
ATCATTG150.00459318758.25335334
CATCATT150.00459318758.25335333
GTTGATA150.00461357858.18826756
CGGAACT150.00461357858.18826754
ATCTACA201.9685384E-458.18826739
CTATATC201.9685384E-458.18826735
TTGTATA201.9685384E-458.18826750
ATAGACA201.9685384E-458.18826754
TATGAAT201.9685384E-458.18826758
CACTCTA150.00461357858.18826755
TATCTAC201.9685384E-458.18826738
CATAGCA150.00461357858.18826749
TCTTTCA150.00461357858.18826750
CCCGGGT150.00461357858.18826757
GCCACTA201.9685384E-458.18826739
AAAGCGC150.00461357858.18826738
Produced by FastQC (version 0.11.5)