FastQCFastQC Report
Fri 17 Jun 2016
SRR1526581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83502
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8060.9652463414049963No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5260.6299250317357669No Hit
CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.5029819645038442No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.4454983114176906No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2430.29101099374865275No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2400.28741826543076815No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.27664008047711436No Hit
CGCTTGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG2250.2694546238413451No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2010.2407127972982683No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1720.20598309022538383No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1630.19520490527173No Hit
CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1590.19041460084788386No Hit
CGCTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1470.17604368757634548No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1440.17245095925846088No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1410.16885823094057628No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1300.1556848937749994No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.15448731766903787No Hit
CGCTTGGGGGATTATTACCAAAATCAAGACTGGAGAGATGGCTCAGTGCTTAAGAG1260.15089458935115327No Hit
CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1240.1484994371392302No Hit
CGCTTGGGGCTAGAGCAGTTTTCATATTGGAAAATGACAATGTCTTTTTTCTTTCA1170.14011640439749945No Hit
CGCTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1120.13412852386769178No Hit
CGCTTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1060.12694306723192259No Hit
CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.12335033891403797No Hit
CGCTTGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1010.12095518670211493No Hit
CGCTTGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT970.11616488227826878No Hit
CGCTTGGGGGTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT970.11616488227826878No Hit
CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT960.11496730617230726No Hit
CGCTTGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATTCCAGCACTTGGGAGGCAG870.10418912121865345No Hit
CGCTTGGGGGTCACGCTTAGGCCAGCGTCCTTTTACTGTCTGGTCGGGACTTGTTG860.10299154511269192No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACTGACTGCTGTTCC860.10299154511269192No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGCGGATAAGGGCATTGACTTCTCTTCCA860.10299154511269192No Hit
CGCTTGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG860.10299154511269192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAC309.62189E-750.5383236
TAAAAGC150.00805509350.5383234
TACTGTC150.00805509350.5383234
GTGAGGT150.00805509350.5383236
ATTTGGC150.00805509350.5383237
AAAGCAC150.00805509350.5383236
GACTATA150.00805509350.5383237
GCTTCGC150.00811340250.44626633
ATAAGGG150.00811340250.44626633
CTAGAAG150.008132908550.41565331
TAGATTA150.008132908550.41565332
GGGTTAG150.008132908550.41565331
GGTCCAC204.066961E-450.2327740
TATAGTT150.00825067950.2327740
GGTAAGG301.0075564E-650.20241545
TAGGTTC150.0082704350.20241546
AGCGCGA150.0082704350.20241547
CTCGAAG150.0082704350.20241547
TAAGGGG252.02167E-550.20241547
TGCTAAT150.0082704350.20241546
Produced by FastQC (version 0.11.5)