##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526581_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83502 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.576477210126704 39.0 38.0 40.0 36.0 40.0 2 38.08794998922181 39.0 38.0 40.0 35.0 40.0 3 38.047807238149986 39.0 38.0 40.0 35.0 40.0 4 38.19488155972312 39.0 38.0 40.0 35.0 40.0 5 38.277634068645064 39.0 38.0 40.0 35.0 40.0 6 37.987736820674954 39.0 38.0 40.0 35.0 40.0 7 38.317465449929344 39.0 38.0 40.0 36.0 40.0 8 38.49829944192953 39.0 38.0 40.0 36.0 40.0 9 38.368075016167275 39.0 38.0 40.0 36.0 40.0 10 37.628296328231656 39.0 38.0 40.0 33.0 40.0 11 37.64484683001605 39.0 38.0 40.0 33.0 40.0 12 37.652643050465855 39.0 38.0 40.0 34.0 40.0 13 37.680402864602044 39.0 38.0 40.0 34.0 40.0 14 37.65016406792652 39.0 38.0 40.0 33.0 40.0 15 37.68633086632656 39.0 38.0 40.0 33.0 40.0 16 37.52231084285406 39.0 38.0 40.0 33.0 40.0 17 37.40946324638931 39.0 38.0 40.0 33.0 40.0 18 37.36145242030131 39.0 37.0 40.0 33.0 40.0 19 37.16078656798639 39.0 37.0 40.0 33.0 40.0 20 37.17388805058562 39.0 37.0 40.0 33.0 40.0 21 37.02193959426121 39.0 36.0 40.0 33.0 40.0 22 36.928935833872245 39.0 36.0 40.0 33.0 40.0 23 36.75788603865776 39.0 36.0 40.0 32.0 40.0 24 36.621182726162246 39.0 36.0 40.0 31.0 40.0 25 36.58632128571771 39.0 36.0 40.0 31.0 40.0 26 36.149481449546116 38.0 36.0 40.0 30.0 40.0 27 35.99201216737324 38.0 35.0 40.0 30.0 40.0 28 35.7185815908601 38.0 35.0 40.0 29.0 40.0 29 35.640787047016836 38.0 35.0 40.0 29.0 40.0 30 35.33118967210366 38.0 35.0 40.0 29.0 40.0 31 34.61527867595986 38.0 34.0 39.0 26.0 40.0 32 34.402337668558836 38.0 33.0 39.0 25.0 40.0 33 34.30432804004695 38.0 33.0 39.0 25.0 40.0 34 34.26462839213432 38.0 33.0 39.0 25.0 40.0 35 34.05743575004192 38.0 33.0 39.0 24.0 40.0 36 33.2906996239611 38.0 33.0 39.0 16.0 40.0 37 32.82317788795478 38.0 33.0 39.0 9.0 40.0 38 32.824914373308424 37.0 32.0 39.0 11.0 40.0 39 32.705216641517566 37.0 32.0 39.0 10.0 40.0 40 32.345392924720365 37.0 32.0 39.0 4.0 40.0 41 31.892445689923594 36.0 31.0 39.0 4.0 40.0 42 31.714964911020097 36.0 31.0 39.0 4.0 40.0 43 31.62180546573735 36.0 31.0 39.0 4.0 40.0 44 31.069950420349212 36.0 30.0 39.0 4.0 40.0 45 30.976874805393884 36.0 30.0 39.0 4.0 40.0 46 30.38449378458001 36.0 29.0 39.0 4.0 40.0 47 30.1774807789035 35.0 29.0 39.0 4.0 40.0 48 29.7192043304352 35.0 28.0 39.0 4.0 40.0 49 29.584477018514526 35.0 27.0 39.0 4.0 40.0 50 29.205659744676773 35.0 27.0 39.0 4.0 40.0 51 28.540070896505473 35.0 25.0 39.0 4.0 40.0 52 28.281981269909704 35.0 24.0 39.0 4.0 40.0 53 27.943222916816364 34.0 22.0 38.0 4.0 40.0 54 27.57072884481809 34.0 18.0 38.0 4.0 40.0 55 27.03546022849752 34.0 4.0 38.0 4.0 40.0 56 16.09058465665493 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.03549923365748242 1 2 0.0707711888430822 1 3 -0.40320046932520626 1 4 0.20210331317452557 1 5 0.4242374733086649 1 6 0.10116778109577496 1 7 -0.27947474151840623 1 8 -0.8078549150560193 1 9 -0.5625893549769785 1 10 0.5426134693795319 1 11 0.31296236064937943 1 12 0.20601048262250998 1 13 -0.27690716298097584 1 14 -0.02441921292021476 1 15 -0.47507208526667455 1 16 -0.05894679585771456 1 17 0.22248652769411592 1 18 -0.3695643399601707 1 19 0.14077497809343242 1 20 0.4220508720624494 1 21 0.8131921805421811 1 22 0.49521978854458837 1 23 0.4930914243025839 1 24 -0.08903891025032351 1 25 1.246707296046111 1 26 0.6685537514888082 1 27 0.7206611909999339 1 28 0.09239710338041363 1 29 0.641063218708247 1 30 1.2624622680283721 1 31 0.9793939048241427 1 32 0.949259802808335 1 33 0.7996966707712616 1 34 0.7975536153170495 1 35 0.4605785175310757 1 36 0.7006350170048705 1 37 2.0727251883803817 1 38 1.7947269241838342 1 39 1.776718054526647 1 40 1.5781996811289218 1 41 1.4143323565878667 1 42 1.6500815438817895 1 43 2.139665769203539 1 44 1.5942372965334641 1 45 2.7747007817237765 1 46 2.198562208437977 1 47 2.307891716025452 1 48 2.8263612594467666 1 49 2.926581648506769 1 50 1.967833086751341 1 51 2.6029521773148154 1 52 2.0035486412696812 1 53 2.219956983548162 1 54 2.278928702104949 1 55 2.6834657522109815 1 56 2.64961361794065 2 1 -0.4614143465894358 2 2 -0.21472263831741145 2 3 -0.28591651870792134 2 4 -0.18524236583782283 2 5 -0.017120551382696192 2 6 -0.600992712731383 2 7 -0.08503029707396337 2 8 -0.3047684953029375 2 9 -0.36505849077944674 2 10 0.42687272863879144 2 11 0.2280858174395064 2 12 -0.19059445564909794 2 13 0.21537678763630197 2 14 0.2996548611538614 2 15 -0.11704739390865626 2 16 -0.5172801291910503 2 17 -1.019797422923162 2 18 0.06253442547193089 2 19 -0.2681756391905168 2 20 0.09026074860565814 2 21 -0.7454497947664578 2 22 -0.27638514972701955 2 23 -1.9235752423640875 2 24 -0.30817471271945607 2 25 -1.0758235681514208 2 26 -1.5397795818445275 2 27 -1.1975486855432749 2 28 -0.6190226497060038 2 29 -1.9793071516621197 2 30 -1.1788957566629819 2 31 -1.526778934682035 2 32 -1.385616740401538 2 33 -0.6200564156484916 2 34 -1.3907179896212227 2 35 -1.2107177787652148 2 36 0.16051156021474355 2 37 -0.10783036717517547 2 38 0.27312198591223336 2 39 -1.0010597232511316 2 40 0.5596811626104028 2 41 -0.7230133224244817 2 42 -1.101461665994755 2 43 -1.2384206505495463 2 44 -0.3779849256887573 2 45 -3.160484403461407 2 46 -2.5190303841546147 2 47 -2.724515691381953 2 48 -1.755428864010021 2 49 -2.874344277419155 2 50 -1.4457471601622416 2 51 -2.660936711574074 2 52 -2.1415130871253787 2 53 -1.8572035102789997 2 54 -2.1732317917222126 2 55 -3.137521902110006 2 56 -2.1852629252692264 3 1 -0.6027203111885697 3 2 -0.07430913244206039 3 3 -0.6327854760186469 3 4 -0.2826691098509144 3 5 0.08789209178255675 3 6 0.08426684401411677 3 7 -0.022111957047187047 3 8 -0.13282144785387828 3 9 -0.48896151026479373 3 10 -0.14162347841163836 3 11 -1.366589178841913 3 12 -0.8239426632944955 3 13 -0.26000622589931766 3 14 -1.1880738313941066 3 15 -0.8873880156548992 3 16 -1.177253355429336 3 17 -1.2311613973393492 3 18 -1.824718289090022 3 19 -1.1769961062439194 3 20 -1.2012757678304595 3 21 -1.1704088904082823 3 22 -1.7102688326066868 3 23 -1.2769888969825587 3 24 -1.1563079865555395 3 25 -0.9398723558949911 3 26 -0.586633664843184 3 27 -0.3343923565234945 3 28 -0.2675426556557312 3 29 -0.5580666340360594 3 30 -0.6481803022527473 3 31 -1.1491201514007585 3 32 -1.6938124863482926 3 33 -0.775939206069431 3 34 -1.2395949790604561 3 35 -0.6707802508758789 3 36 -0.78698211820263 3 37 -0.26847293745629486 3 38 -0.713639876351639 3 39 -0.5325188932645233 3 40 -0.544290278710438 3 41 -0.6772004412702373 3 42 -0.6666855243913048 3 43 0.02068986558908037 3 44 -0.17450433666600063 3 45 -0.7437061794542643 3 46 -0.9076961583625582 3 47 -0.9837749506412123 3 48 -0.7979693965103962 3 49 -0.3808146031665913 3 50 -0.46121644470782996 3 51 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36 0.10142866779851545 4 37 0.40407439472959084 4 38 0.03679041624732804 4 39 0.229099006907596 4 40 0.6496282525574912 4 41 0.46393553119103714 4 42 0.5727005915008334 4 43 0.8241895787273492 4 44 1.3997928520890213 4 45 1.2072404642634602 4 46 1.8870542719300403 4 47 1.2424155255492622 4 48 1.32437713246264 4 49 0.5118991088242311 4 50 1.8289441978624517 4 51 1.4898569392195746 4 52 0.9559295936506338 4 53 2.215988729579909 4 54 1.4713890195652652 4 55 1.8977514664966968 4 56 1.7388993322263655 5 1 -0.5131118774536318 5 2 0.2624378555097451 5 3 -0.2684782471029834 5 4 -0.31595224238103015 5 5 0.4909041399753278 5 6 -0.6752211077931136 5 7 0.3635808140371495 5 8 0.10603397383286506 5 9 0.1679662005785758 5 10 -0.7282198639538038 5 11 -0.9634265282395091 5 12 -1.3745450729330457 5 13 -1.26301827409209 5 14 -1.078585879586882 5 15 -0.9861831963777874 5 16 -0.9936690180799346 5 17 -1.7664023611947712 5 18 -1.2584532288490564 5 19 -1.6842250219065704 5 20 -0.8460046834931063 5 21 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6 48 1.6258057038912135 6 49 0.7004705373956597 6 50 1.0856108645291158 6 51 0.34795217731481287 6 52 1.4002153079363495 6 53 0.6588458724370483 6 54 0.5961509243271728 6 55 1.130132418877647 6 56 0.9046136179406492 7 1 -0.08811187745363469 7 2 0.4874378555097465 7 3 -0.40597824710298624 7 4 0.37154775761896985 7 5 -0.14659586002466796 7 6 -0.37522110779310935 7 7 0.26358081403714806 7 8 0.13103397383286364 7 9 0.20546620057857723 7 10 0.34678013604619906 7 11 -0.4259265282395077 7 12 0.13795492706695 7 13 -0.23801827409209153 7 14 -0.04108587958688048 7 15 -0.5236831963777888 7 16 -0.4436690180799374 7 17 -0.5164023611947712 7 18 -0.970953228849055 7 19 -0.13422502190656616 7 20 0.22899531650688942 7 21 -0.2812522639022603 7 22 -0.6575579892331902 7 23 -0.4027419090307518 7 24 -0.06820557691698781 7 25 -0.08384825950944474 7 26 0.37827597371102684 7 27 -0.6140610312222847 7 28 0.7173971033804136 7 29 -0.035325670180640145 7 30 0.020795601361704996 7 31 -0.4414394285091916 7 32 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12805.0 38 14589.0 39 9481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.19961658279415 0.5703330936975797 0.15456506110711718 0.07548526240115025 8 99.08744700725731 0.6514814016430743 0.09460851237096117 0.1664630787286532 9 93.6205120835429 3.4286603913678713 1.410744652822687 1.5400828722665325 10 47.33898589255347 33.41596608464468 7.251323321597088 11.99372470120476 11 32.09144691145122 27.569399535340473 19.625877224497618 20.713276328710688 12 38.12244018107351 16.59002179588513 24.826950252688558 20.460587770352806 13 25.607769873775478 15.950516155301669 29.319058226150275 29.12265574477258 14 24.519173193456446 22.217431917798375 31.06512418864219 22.19827070010299 15 31.80762137433834 18.85463821225839 28.04363967330124 21.294100740102035 16 30.817225934708155 20.389930780101075 25.315561303920862 23.47728198126991 17 30.98967689396661 22.801849057507603 22.497664726593374 23.71080932193241 18 32.38605063351776 21.530023232976454 23.090464899044335 22.993461234461453 19 26.70235443462432 25.352686163205675 24.728749011999714 23.216210390170293 20 27.169409115949318 26.910732677061628 21.94677971785107 23.973078489137983 21 27.096356973485662 22.821010275202987 27.15264305046586 22.92998970084549 22 26.567028334650665 21.139613422432994 26.86761993724701 25.425738305669327 23 30.641916167664675 24.123353293413174 23.635928143712576 21.59880239520958 24 27.8735838662547 26.596967737299703 22.75993389379895 22.769514502646643 25 27.69031701531162 20.849208060767257 23.143555459708146 28.316919464212976 26 26.34000790883273 20.523420929647337 25.83312362943524 27.303447532084697 27 23.371220295901974 19.50459199577968 27.134738508021005 29.98944920029734 28 23.72554191444466 26.326011893343566 25.419624016880878 24.5288221753309 29 28.908105255583006 22.725419175322028 26.08841660869774 22.278058960397225 30 25.2367634526053 23.22742494988657 29.4114822772503 22.12432932025783 31 27.924229328723708 21.62441179295112 29.892922308652647 20.558436569672526 32 27.87641448237753 20.136703265021744 32.591355740816375 19.39552651178435 33 27.706283118849356 20.717830407459477 31.4228037922209 20.153082681470266 34 22.645341152154582 23.56461342498017 37.09894493979668 16.691100483068567 35 22.059283121378943 29.646368728514076 33.28244825347982 15.01189989662716 36 24.79166917184738 27.21708493163863 30.492658818436524 17.498587078077467 37 24.17559744153023 26.684305705581785 31.439564040634522 17.70053281225347 38 24.509615153192616 25.43743531733616 32.31967075019856 17.733278779272666 39 24.51951427918116 22.468800019255532 32.66099430758307 20.350691393980238 40 23.342056705941943 22.382083720257118 32.57033972913325 21.705519844667688 41 20.134874759152215 24.598988439306357 32.64210019267823 22.6240366088632 42 20.394174125697216 22.372272886072594 34.1336481586334 23.09990482959679 43 21.690392102503527 20.345214131600834 35.027662934079046 22.936730831816593 44 21.67868425494972 21.848698965442402 34.56845202208986 21.90416475751803 45 21.472067140152657 23.319385995586693 33.65327802631103 21.55526883794962 46 21.168622815165076 23.19754888360816 33.3735419355617 22.26028636566507 47 20.956148391142843 22.380481451107144 34.82801871773844 21.83535144001158 48 21.725532814103726 23.61407957495623 34.5734468393407 20.086940771599348 49 24.07736047227554 23.566694433378004 32.97236608598023 19.38357900836623 50 20.18492588479552 23.909999517164792 34.31147699290232 21.593597605137365 51 20.71791529372005 25.141519112623868 34.8975872349157 19.24297835874039 52 20.398009950248756 25.264454426894652 33.32125778872627 21.01627783413032 53 22.597593970431927 22.914049666634455 35.49497535993816 18.993381002995456 54 20.747925449033083 23.085192477261472 34.15188008068705 22.015001993018394 55 21.81323991302247 23.68204880405895 31.245469920270597 23.259241362647984 56 21.489490215027786 25.16791495530321 30.294757187726507 23.047837641942497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 10.0 9 15.0 10 47.0 11 79.0 12 275.5 13 297.0 14 122.0 15 113.0 16 104.0 17 146.0 18 188.0 19 328.0 20 468.0 21 581.0 22 741.0 23 788.0 24 990.5 25 1193.0 26 1492.0 27 1791.0 28 2068.5 29 2607.5 30 2869.0 31 2942.0 32 3015.0 33 2934.0 34 2853.0 35 2480.5 36 2108.0 37 2242.5 38 1886.5 39 1396.0 40 1260.5 41 1125.0 42 1224.0 43 1323.0 44 1411.5 45 1500.0 46 1717.0 47 1758.5 48 1583.0 49 1664.0 50 1745.0 51 1923.5 52 2102.0 53 2275.5 54 2893.0 55 3337.0 56 3664.5 57 3992.0 58 4509.5 59 5027.0 60 5334.0 61 5641.0 62 6279.0 63 6625.5 64 6334.0 65 5368.5 66 4403.0 67 3978.5 68 3554.0 69 3091.0 70 2628.0 71 2164.5 72 1456.0 73 1211.0 74 970.0 75 729.0 76 647.5 77 566.0 78 452.0 79 270.0 80 202.0 81 167.0 82 132.0 83 99.5 84 67.0 85 51.5 86 36.0 87 30.0 88 16.5 89 9.0 90 7.5 91 6.0 92 4.5 93 3.0 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05029819645038442 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.002395152211923068 24 0.0 25 0.04311273981461522 26 0.061076381404038226 27 0.11496730617230726 28 0.11257215396038417 29 0.1484994371392302 30 0.22873703623865296 31 0.23712006898038368 32 0.3065794831261527 33 0.33412373356326797 34 0.3401116140930756 35 0.3688534406361524 36 0.408373452132883 37 1.328111901511341 38 0.4838207468084597 39 0.48980862733826736 40 0.6981868697755742 41 0.5532801609542287 42 0.5904050202390362 43 0.6454935211132667 44 0.6802232281861512 45 0.6838159565040358 46 0.7197432396828818 47 0.7017795980934588 48 0.8227347847955738 49 0.801178414888266 50 0.7880050777226894 51 0.7796220449809586 52 0.8263275131134583 53 0.850279035232689 54 0.8538717635505736 55 0.8646499485042274 56 0.8646499485042274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 83502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.402505329213675 #Duplication Level Percentage of deduplicated Percentage of total 1 72.33329816416966 32.84112955378314 2 11.394809031441232 10.347057555507652 3 5.151403249630724 7.016598404828627 4 2.9462966870647813 5.350770041436133 5 1.8727579658155729 4.251395176163445 6 1.4718294998944925 4.009484802759215 7 0.9416543574593795 2.992742688797873 8 0.6989871280860941 2.538861344638452 9 0.5301751424351129 2.1664151756844148 >10 2.48470141380038 18.755239395463583 >50 0.11078286558345643 3.457402217910948 >100 0.058029120067524795 4.677732269885751 >500 0.005275374551593163 1.595171373140763 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 806 0.9652463414049963 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 526 0.6299250317357669 No Hit CGCTTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 420 0.5029819645038442 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 372 0.4454983114176906 No Hit CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 243 0.29101099374865275 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 240 0.28741826543076815 No Hit CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 0.27664008047711436 No Hit CGCTTGGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG 225 0.2694546238413451 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 201 0.2407127972982683 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 172 0.20598309022538383 No Hit CGCTTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 163 0.19520490527173 No Hit CGCTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 159 0.19041460084788386 No Hit CGCTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 147 0.17604368757634548 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 144 0.17245095925846088 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC 141 0.16885823094057628 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 130 0.1556848937749994 No Hit CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.15448731766903787 No Hit CGCTTGGGGGATTATTACCAAAATCAAGACTGGAGAGATGGCTCAGTGCTTAAGAG 126 0.15089458935115327 No Hit CGCTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 124 0.1484994371392302 No Hit CGCTTGGGGCTAGAGCAGTTTTCATATTGGAAAATGACAATGTCTTTTTTCTTTCA 117 0.14011640439749945 No Hit CGCTTGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 112 0.13412852386769178 No Hit CGCTTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 106 0.12694306723192259 No Hit CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.12335033891403797 No Hit CGCTTGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 101 0.12095518670211493 No Hit CGCTTGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT 97 0.11616488227826878 No Hit CGCTTGGGGGTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT 97 0.11616488227826878 No Hit CGCTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 96 0.11496730617230726 No Hit CGCTTGGGGGCCGGGCGTGGTGGCGCACGCCTTTAATTCCAGCACTTGGGAGGCAG 87 0.10418912121865345 No Hit CGCTTGGGGGTCACGCTTAGGCCAGCGTCCTTTTACTGTCTGGTCGGGACTTGTTG 86 0.10299154511269192 No Hit CGCTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACTGACTGCTGTTCC 86 0.10299154511269192 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGCGGATAAGGGCATTGACTTCTCTTCCA 86 0.10299154511269192 No Hit CGCTTGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 86 0.10299154511269192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.013173337165576872 0.0 0.0 0.0 0.0 11 0.023951522119230677 0.0 0.0 0.0 0.0 12 0.023951522119230677 0.0 0.0 0.0 0.0 13 0.027544250437115277 0.0 0.0 0.0 0.0 14 0.033532130966922945 0.0 0.0 0.0 0.0 15 0.03472970707288448 0.0 0.0 0.0 0.0 16 0.03592728317884602 0.0 0.0 0.0 0.0 17 0.039520011496730616 0.0 0.0 0.0 0.0 18 0.04311273981461522 0.0 0.0 0.0 0.0 19 0.04910062034442289 0.0 0.0 0.0 0.0 20 0.061076381404038226 0.0 0.0 0.0 0.0 21 0.06466910972192283 0.0 0.0 0.0 0.0 22 0.07185456635769204 0.0 0.0 0.0 0.0 23 0.0814351752053843 0.0 0.0 0.0 0.0 24 0.09820124068884578 0.0 0.0 0.0 0.0 25 0.10778184953653805 0.0 0.0 0.0 0.0 26 0.11137457785442265 0.0 0.0 0.0 0.0 27 0.11975761059615339 0.0 0.0 0.0 0.0 28 0.1377212521855764 0.0 0.0 0.0 0.0 29 0.14370913271538407 0.0 0.0 0.0 0.0 30 0.1520921654571148 0.0 0.0 0.0 0.0 31 0.1556848937749994 0.0 0.0 0.0 0.0 32 0.16167277430480706 0.0 0.0 0.0 0.0 33 0.16526550262269168 0.0 0.0 0.0 0.0 34 0.1736485353644224 0.0 0.0 0.0 0.0 35 0.2407127972982683 0.0 0.0 0.0 0.0 36 0.2478982539340375 0.0 0.0 0.0 0.0 37 0.2526885583578836 0.0 0.0 0.0 0.0 38 0.2622691672055759 0.0 0.0 0.0 0.0 39 0.26705947162942206 0.0 0.0 0.0 0.0 40 0.2766400804771143 0.0 0.0 0.0 0.0 41 0.2934061459605758 0.0 0.0 0.0 0.0 42 0.300591602596345 0.0 0.0 0.0 0.0 43 0.30897463533807573 0.0 0.0 0.0 0.0 44 0.3113697875499988 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAAC 30 9.62189E-7 50.53832 36 TAAAAGC 15 0.008055093 50.53832 34 TACTGTC 15 0.008055093 50.53832 34 GTGAGGT 15 0.008055093 50.53832 36 ATTTGGC 15 0.008055093 50.53832 37 AAAGCAC 15 0.008055093 50.53832 36 GACTATA 15 0.008055093 50.53832 37 GCTTCGC 15 0.008113402 50.446266 33 ATAAGGG 15 0.008113402 50.446266 33 CTAGAAG 15 0.0081329085 50.415653 31 TAGATTA 15 0.0081329085 50.415653 32 GGGTTAG 15 0.0081329085 50.415653 31 GGTCCAC 20 4.066961E-4 50.23277 40 TATAGTT 15 0.008250679 50.23277 40 GGTAAGG 30 1.0075564E-6 50.202415 45 TAGGTTC 15 0.00827043 50.202415 46 AGCGCGA 15 0.00827043 50.202415 47 CTCGAAG 15 0.00827043 50.202415 47 TAAGGGG 25 2.02167E-5 50.202415 47 TGCTAAT 15 0.00827043 50.202415 46 >>END_MODULE