FastQCFastQC Report
Fri 17 Jun 2016
SRR1526580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216149
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC31431.454089540085774No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22091.0219802080971923No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC12550.580617999620632No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC11990.5547099454542931No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9630.44552600289615035No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9110.421468524027407No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG8820.40805185311983866No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC8300.3839943742510953No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.29840526673729695No Hit
GACACTGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCCTGA6120.28313802053213294No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCAGAGGTCCT5780.26740813050257No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT4830.22345696718467353No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC4520.20911500862830731No Hit
GACACTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4480.2072644333307117No Hit
GACACTGGGGTTTTCTTTAGATTTTTAAAAAAAGATTTGTGTGTGTGTTTTGTGTGTATTGGGT4230.1956983377207389No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.18320695446196839No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3880.17950580386677709No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC3850.17811787239358035No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3850.17811787239358035No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCC3650.16886499590560217No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGGTCC3520.16285062618841634No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3450.15961211941762396No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC3380.15637361264683158No Hit
GACACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG3340.15452303734923595No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC3230.14943395528084794No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC3070.14203165409046536No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCCT3040.14064372261726865No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC3000.13879314731967302No Hit
GACACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG2920.13509199672448172No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCTTCCGAAGGTCC2770.12815233935849807No Hit
GACACTGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATACCCAG2740.12676440788530133No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT2580.11936210669491878No Hit
GACACTGGGGCCAGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAAAGGCAGGC2520.11658624374852533No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCC2480.1147356684509297No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2450.11334773697773295No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2450.11334773697773295No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG2440.11288509315333405No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCAAAGGTC2350.10872129873374385No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTC2330.10779601108494602No Hit
GACACTGGGCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACT2300.1064080796117493No Hit
GACACTGGGGGTCCCTTCCTCCCAGTGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTC2170.10039370989456348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGT501.8189894E-1258.2354358
ACTCGAT150.004607558358.23542854
ATGCACA150.004607558358.23542853
CCACACG150.004607558358.23542857
TTCTTAT201.9691579E-458.22186351
ACTTGCA150.00461181358.2218651
TAAGTCG150.00461181358.2218648
TATGCAC150.00461181358.2218652
ACGGGGT150.00461181358.2218650
AGACCGG150.00461181358.2218650
AGCGTGA150.00462886258.16763742
AAGCCGC150.00462886258.16763743
GTGCGAA150.00462886258.16763743
GTAAACC150.00462886258.16763743
AACGTTC150.004633131458.154140
CGCATAC258.5108295E-658.154140
CCACTTA258.5108295E-658.154140
GCATACA258.5108295E-658.154141
CCGCATA258.522584E-658.14056439
GATACGT201.9850944E-458.12703737
Produced by FastQC (version 0.11.5)