FastQCFastQC Report
Fri 17 Jun 2016
SRR1526579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209650
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC24561.1714762699737657No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22991.0965895540186024No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10750.5127593608394944No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9830.46887669926067255No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8270.3944669687574529No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT7590.3620319580252802No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG7170.3419985690436442No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.2652039112807059No Hit
GACACTGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCCTGA5400.2575721440496065No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC5140.24517052229906985No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCAGAGGTCCT4850.23133794419270215No Hit
GACACTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4210.20081087526830432No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT3810.1817314571905557No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC3290.15692821368948245No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3260.15549725733365133No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC3210.15311233007393274No Hit
GACACTGGGGTTTTCTTTAGATTTTTAAAAAAAGATTTGTGTGTGTGTTTTGTGTGTATTGGGT3170.1512043882661579No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCC3150.15025041736227046No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.14643453374672072No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG3070.14643453374672072No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3070.14643453374672072No Hit
GACACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG3010.14357262103505844No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2850.13594085380395898No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGGTCC2840.13546386835201527No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC2800.1335559265442404No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC2680.1278321011209158No Hit
GACACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG2360.11256856665871691No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGAGGTCCT2270.10827569759122348No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCTTCCGAAGGTCC2240.10684474123539232No Hit
GACACTGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATACCCAG2200.10493679942761747No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC2110.100643930360124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAT150.004616990758.20471237
GCTTAAA258.478182E-658.19071639
CGCACAT150.004621390758.19071639
CAATATC150.004621390758.19071655
TGATGTC150.004621390758.19071655
CCAAAAC150.00462579458.17672335
TAGCCAG201.98364E-458.13479247
CTTATCT201.98364E-458.13479247
TACGTGG201.98364E-458.13479248
ATTCGTC150.00463902258.1347947
GTAGGCT201.985998E-458.12082745
GTAATGT201.985998E-458.12082745
ATGGGTA150.004647856658.10686530
CCTAAGT150.004647856658.10686551
AATAGAC150.004647856658.10686530
ACACCTA150.004656703758.0789742
ACATTCG150.004656703758.0789742
CTACTAA407.1122486E-1058.02326225
CGACCCT150.004674435658.02326225
AAACCGT150.004674435658.02326226
Produced by FastQC (version 0.11.5)