SRR1526578_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193962
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	2813	1.4502840762623606	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1879	0.9687464554912819	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1634	0.8424330538971551	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	1538	0.7929388230684361	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	1231	0.6346603973974283	No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1028	0.5300007217908662	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	951	0.49030222414699787	No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	902	0.4650395438281725	No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	724	0.37326899083325604	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	619	0.3191346758643446	No Hit
GACACTGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCAGA	514	0.2650003608954331	No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA	479	0.24695558923912933	No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.22478629834709893	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC	408	0.21035048102205586	No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC	395	0.20364813726400013	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	362	0.18663449541662802	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC	323	0.1665274641424609	No Hit
GACACTGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCC	322	0.16601189923799506	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGCTAAGAGCACCCGACTGCTCTTCC	316	0.16291850981120015	No Hit
GACACTGGGGTTTTCTTTAGATTTTTAAAAAAAGATTTGTGTGTGTGTTTTGTGTG	308	0.15879399057547355	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC	289	0.14899825739062292	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC	280	0.14435817325043052	No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT	276	0.1422959136325672	No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	273	0.14074921891916972	No Hit
GACACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	265	0.13662469968344315	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCTTCC	254	0.1309534857343191	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC	254	0.1309534857343191	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	241	0.12425114197626339	No Hit
GACACTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCG	238	0.12270444726286592	No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTCA	238	0.12270444726286592	No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	236	0.12167331745393428	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	225	0.11600210350481022	No Hit
GACACTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAAAGCACTGACTGCTCTTTC	220	0.1134242789824811	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	195	0.10053515637083552	No Hit
GACACTGGGGCCAGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAA	194	0.1000195914663697	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTCT	25	1.9459809E-5	50.56978	37
TGCGCAC	15	0.008068198	50.543312	36
CTTACGA	15	0.008068198	50.543312	36
CGTTCGT	20	3.9685098E-4	50.516876	35
ACTTACG	15	0.00808491	50.516872	35
ATAACTC	15	0.00808491	50.516872	35
ACTACTG	20	4.0045116E-4	50.42454	33
CTTGACA	20	4.0045116E-4	50.42454	33
AACGAGC	15	0.008143604	50.42454	33
TCATAAC	15	0.008143604	50.42454	33
TAGACTT	15	0.008152015	50.41138	32
ATACTAA	35	4.950016E-8	50.38507	31
TTATCTG	15	0.008304514	50.17563	48
TTATCTC	15	0.008304514	50.17563	47
TATCTCA	15	0.008304514	50.17563	48
CAATTGC	20	4.1088057E-4	50.162598	46
AGACCTA	15	0.008313048	50.162598	50
AGATTAC	20	4.1088057E-4	50.162598	50
ATTATCT	15	0.008313048	50.162598	46
GTGCCCG	15	0.008313048	50.162598	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content