FastQCFastQC Report
Fri 17 Jun 2016
SRR1526577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111938
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.9916203612714182No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.7959763440475979No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5700.5092104557880255No Hit
TGGATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.376101055941682No Hit
TGGATGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2220.1983240722542836No Hit
TGGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.19385731387017813No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2130.19028390716289376No Hit
TGGATGGGGGAGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.18939055548607267No Hit
TGGATGGGGGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.18939055548607267No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1880.1679501152423663No Hit
TGGATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1720.15365648841322876No Hit
TGGATGGGGATTCGTGAAACCCATAAACATGGTCAACTTGGAAAAAGAAACCAACGGGTATTCC1670.14918973002912328No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1430.12774928978541694No Hit
TGGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1360.12149582804766923No Hit
TGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.12149582804766923No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1290.11524236630992156No Hit
TGGATGGGGACAGCTATGGGACAGCAGCCATGGGCTGTGTCTACATGGGCACTGCGGCCGCCCT1260.11256231127945827No Hit
TGGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.11166895960263719No Hit
TGGATGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1220.10898890457217388No Hit
TGGATGGGGGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATACATGCAAC1180.10541549786488949No Hit
TGGATGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACG1160.10362879451124729No Hit
TGGATGGGGAGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.10094873948078399No Hit
TGGATGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.10094873948078399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT201.9781482E-458.14356255
ACATCTA201.9781482E-458.14356256
TATACAT258.493202E-658.14356253
ATGCAAC258.493202E-658.14356258
CCCTAGA150.004630613658.1435654
GATCATG150.004630613658.1435652
TCCTAAT150.004630613658.1435653
TTGAACG150.004630613658.1435657
GATCTTA150.004630613658.1435652
ATTTCTT150.004630613658.1435654
GTTGCTC150.004630613658.1435654
CTGTTAG150.00463884958.1174948
TGTTAGA150.00463884958.1174949
GTTGGCA150.00463884958.1174949
TAGACAA150.00464709558.0914441
GAGTAAG150.00464709558.0914439
AGACGCA201.9869447E-458.0914439
CCAAGAG201.9869447E-458.0914440
AAGACGC201.9957723E-458.0394138
CGACCAA150.0046636258.0394137
Produced by FastQC (version 0.11.5)