FastQCFastQC Report
Fri 17 Jun 2016
SRR1526576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108512
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.8155780005897966No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.6109923326452373No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4770.4395827189619581No Hit
TGGATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.34281922736655857No Hit
TGGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.18615452668829252No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2010.18523296962547922No Hit
TGGATGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1890.17417428487171926No Hit
TGGATGGGGGAGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.1502138012385727No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1500.1382335594219994No Hit
TGGATGGGGGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.13270421704511942No Hit
TGGATGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1430.1317826599823061No Hit
TGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.12809643173105278No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1380.12717487466823946No Hit
TGGATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1290.11888086110291948No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1230.11335151872603952No Hit
TGGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.10321439103509289No Hit
TGGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1110.10229283397227956No Hit
TGGATGGGGATTCGTGAAACCCATAAACATGGTCAACTTGGAAAAAGAAACCAACGGGTATTCC1090.10044971984665291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTAC150.004611427458.20324738
CCGATGT150.004611427458.20324739
GTTTACC150.004611427458.20324739
AGGAATT150.004611427458.20324739
GTAGGAT150.004611427458.20324733
TACGGAC258.4398125E-658.20324739
GTACGGA258.4398125E-658.20324738
GACCGTA150.004636911758.12233748
AACCAAC201.9814372E-458.12233749
CCGTATA150.004636911758.12233750
AGTGACC150.004636911758.12233735
CATGTCG201.9814372E-458.12233745
AAGATTA351.5959813E-858.12233748
CAACTAA150.004636911758.12233745
AAAGATT351.5959813E-858.12233747
CGGGTAT201.985984E-458.09541355
CGGAAAC150.0046454358.09541356
GATGTAG150.0046454358.09541341
CTGTAGT150.0046454358.09541342
CGTATAG150.0046454358.09541351
Produced by FastQC (version 0.11.5)