FastQCFastQC Report
Fri 17 Jun 2016
SRR1526575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98524
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10691.0850148187243718No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.6475579554220292No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6350.6445130120579757No Hit
TGGATGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4850.49226584385530425No Hit
TGGATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.2578052048231903No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2230.2263407900613049No Hit
TGGATGGGGGAGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2100.21314603548373998No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1940.19690633754212172No Hit
TGGATGGGGGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.18675652632861028No Hit
TGGATGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1760.17863667735780114No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1680.170516828386992No Hit
TGGATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1520.15427713044537372No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1450.1471722625959157No Hit
TGGATGGGGATTCGTGAAACCCATAAACATGGTCAACTTGGAAAAAGAAACCAACG1390.14108237586780886No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1300.13194754577564857No Hit
TGGATGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1250.12687264016889285No Hit
TGGATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1210.12281271568348828No Hit
TGGATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12281271568348828No Hit
TGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.12078275344078601No Hit
TGGATGGGGACAGCTATGGGACAGCAGCCATGGGCTGTGTCTACATGGGCACTGCG1170.11875279119808371No Hit
TGGATGGGGAGTGCAGTGCTTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.11773781007673256No Hit
TGGATGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1080.10961796110592344No Hit
TGGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG1060.10758799886322115No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1050.10657301774186999No Hit
TGGATGGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGA1040.10555803662051885No Hit
TGGATGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA990.10048313101376313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGC203.9251425E-450.60413436
GGCAAAC150.00801803150.60413436
AGCGATC150.00801803150.60413435
CGATCGC150.00801803150.60413437
AAATGCG203.9251425E-450.60413437
TATCATG203.9351723E-450.57799534
ATATGCT950.050.57799534
CATATGC950.050.49974433
CTATCAT203.965384E-450.49974433
CGTTAAA402.417437E-950.49974431
TATAAGA203.965384E-450.49974431
TAGACCT150.00808379750.4997431
ACACTGC150.00808379750.4997431
ATTTGTG150.00808379750.4997432
CTCACAA252.0213876E-550.21487445
AGAAACC204.0777406E-450.21487446
CGGGGAT204.0777406E-450.21487442
AAGATTA1000.050.21487448
AAAGATT1000.050.21487447
GTCATCG150.00826672250.2148748
Produced by FastQC (version 0.11.5)