FastQCFastQC Report
Fri 17 Jun 2016
SRR1526574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109884
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.47959666557460595No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4640.4222634778493684No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.2875759892250009No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3060.27847548323686794No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.25299406647009576No Hit
TGTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC2760.25117396527246916No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT2390.21750209311637725No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.19657092934367149No Hit
TGTGCGGGGTGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTAAAATTGTGTTTTTTCTTT2070.18838047395435187No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2000.1820101197626588No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1970.17927996796621892No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1950.17745986676859232No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.17654981616977905No Hit
TGTGCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1900.17290961377452585No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1810.1647191583852062No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1640.14924829820538021No Hit
TGTGCGGGGGTGCCACCGACAGGACGGTGACTGACTGGAATTGCAACTTGTCCTTTTTTCTTGG1610.14651814640894031No Hit
TGTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC1550.14105784281606057No Hit
TGTGCGGGGAGTGTGCCTGTACCAGAGTTTGCGGACAGCGATCCTGCCAACATTGTGCACGACT1550.14105784281606057No Hit
TGTGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTCCTCTCCCC1530.13923774161843397No Hit
TGTGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1480.1346874886243675No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1380.12558698263623458No Hit
TGTGCGGGGGGTGCCTCCGTTTTCCCTTCCTAGTGACCCCAGGTTTAGCATCGTCCTATACTGT1360.12376688143860798No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1340.1219467802409814No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1330.1210367296421681No Hit
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.11375632485166175No Hit
TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1250.11375632485166175No Hit
TGTGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1240.11284627425284846No Hit
TGTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1220.11102617305522187No Hit
TGTGCGGGGAACCTCATTGCAACCATTACAATTTTTTAACATCATGGCTAGCCCTTTTCCGGCG1220.11102617305522187No Hit
TGTGCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1210.11011612245640856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCCG258.4705935E-658.16842358
CATAGCC150.004622540458.1684257
GTCTCAA501.8189894E-1258.14181542
ATTAAGC501.8189894E-1258.14181551
TTCGAGA258.49362E-658.14181543
CTAGACA201.9782725E-458.1418149
GGTTTAG201.9782725E-458.1418142
TCCTATA201.9782725E-458.1418154
CGTTCGA201.9782725E-458.1418141
ATCGTCC201.9782725E-458.1418150
ACTAGAC201.9782725E-458.1418148
CCCAAGG201.9782725E-458.1418154
CACTAGA201.9782725E-458.1418147
CATCGTC201.9782725E-458.1418149
CCTATAC201.9782725E-458.1418155
AGGTATT150.00463092758.14180852
ACACGCG150.00463092758.14180852
CTACTCA150.00463092758.14180849
GTATTAA150.00463092758.14180854
GGTAAGT150.00463092758.14180849
Produced by FastQC (version 0.11.5)