FastQCFastQC Report
Fri 17 Jun 2016
SRR1526573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104906
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.44420719501267797No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4020.38320019827273943No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2950.28120412559815455No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2250.2144777229138467No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.2125712542657236No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.19922597372886205No Hit
TGTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC1990.1896936304882466No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1870.17825481859950812No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.15728366347015424No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1640.1563304291460927No Hit
TGTGCGGGGTGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTAAAATTGTGTTTTTTCTTT1600.15251749184984653No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1530.14584485158141575No Hit
TGTGCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1480.14107867996110804No Hit
TGTGCGGGGGTGCCACCGACAGGACGGTGACTGACTGGAATTGCAACTTGTCCTTTTTTCTTGG1430.13631250834080033No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.1334528053686157No Hit
TGTGCGGGGAGTGTGCCTGTACCAGAGTTTGCGGACAGCGATCCTGCCAACATTGTGCACGACT1340.12773339942424647No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1330.12678016510018492No Hit
TGTGCGGGGGGTGCCTCCGTTTTCCCTTCCTAGTGACCCCAGGTTTAGCATCGTCCTATACTGT1210.11534135321144642No Hit
TGTGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1190.11343488456332336No Hit
TGTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1180.11248165023926182No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1120.10676224429489256No Hit
TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1070.10199607267458487No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1060.10104283835052333No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1060.10104283835052333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCT150.004535447358.44632339
TATGCTG201.9273556E-458.44632339
ACAATTT150.004535447358.44632339
TTATGCT201.9319713E-458.4180838
TTTATGC201.9319713E-458.4180837
CGTGATC150.004544142658.41807638
AACGCTT150.004544142658.41807636
GAAACGT150.004544142658.41807637
ACGTGAT150.004544142658.41807637
TCCAACC150.004544142658.41807638
GCGTAGA150.004544142658.41807638
TACGTGA150.004544142658.41807636
ATATGCT406.712071E-1058.3616634
TCCTACG150.004561570558.3616633
AAGTTTA150.004561570558.3616634
AGTTTAT150.004579047758.30535535
TGATAGC150.004605357558.221158
CAATTTA150.004605357558.221140
TAGGCTC351.5737896E-858.22109641
TTCTACC201.9692151E-458.1930752
Produced by FastQC (version 0.11.5)