SRR1526572_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96540
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	597	0.6183965195773773	No Hit
TGTGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	544	0.5634969960638078	No Hit
TGTGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	324	0.3356121814791796	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	273	0.28278433809819764	No Hit
TGTGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.26103169670602855	No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	223	0.23099233478350942	No Hit
TGTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	195	0.20198881292728404	No Hit
TGTGCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	193	0.19991713279469647	No Hit
TGTGCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	178	0.18437953180029004	No Hit
TGTGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	178	0.18437953180029004	No Hit
TGTGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	177	0.18334369173399628	No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	175	0.18127201160140877	No Hit
TGTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	172	0.17816449140252744	No Hit
TGTGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA	167	0.17298529107105862	No Hit
TGTGCGGGGTGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTAAAATTGTGTT	167	0.17298529107105862	No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	148	0.15330432981147712	No Hit
TGTGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	145	0.15019680961259582	No Hit
TGTGCGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	138	0.14294592914853949	No Hit
TGTGCGGGGGTGCCACCGACAGGACGGTGACTGACTGGAATTGCAACTTGTCCTTT	133	0.13776672881707064	No Hit
TGTGCGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	123	0.127408328154133	No Hit
TGTGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	122	0.12637248808783924	No Hit
TGTGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	122	0.12637248808783924	No Hit
TGTGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	121	0.12533664802154548	No Hit
TGTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	120	0.1243008079552517	No Hit
TGTGCGGGGGGTGCCTCCGTTTTCCCTTCCTAGTGACCCCAGGTTTAGCATCGTCC	119	0.12326496788895795	No Hit
TGTGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC	118	0.12222912782266418	No Hit
TGTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG	113	0.11704992749119536	No Hit
TGTGCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	106	0.10979904702713901	No Hit
TGTGCGGGGAGTGTGCCTGTACCAGAGTTTGCGGACAGCGATCCTGCCAACATTGT	106	0.10979904702713901	No Hit
TGTGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT	105	0.10876320696084525	No Hit
TGTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT	104	0.10772736689455148	No Hit
TGTGCGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTC	103	0.10669152682825772	No Hit
TGTGCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	101	0.10461984669567019	No Hit
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98	0.10151232649678889	No Hit
TGTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	98	0.10151232649678889	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAGCG	15	0.008043829	50.562336	37
TCGCTTG	15	0.008043829	50.562336	37
AAGTGCA	20	3.9613486E-4	50.509197	35
ACCCATC	20	3.9613486E-4	50.509197	34
CCTCGCT	15	0.008077358	50.509193	35
ACATGCT	15	0.008077358	50.509193	34
GCGTGTA	15	0.008077358	50.509193	34
AAGGCTA	15	0.008077358	50.509193	36
AGTCAGC	15	0.008127846	50.429695	33
CACATGC	15	0.008127846	50.429695	33
GTAGGGC	20	3.99229E-4	50.429695	33
GTTAAAG	15	0.008144727	50.403248	32
CGTTAAA	15	0.008161634	50.37683	31
TCCCAAC	15	0.008161634	50.37683	31
ATGTGTA	20	4.0968184E-4	50.166496	48
CATAATG	20	4.0968184E-4	50.166496	44
CTTGTCG	15	0.0082978355	50.16649	45
AATGGCC	15	0.0082978355	50.16649	47
ACGATAC	15	0.0082978355	50.16649	45
AACGATA	15	0.0082978355	50.16649	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content