FastQCFastQC Report
Fri 17 Jun 2016
SRR1526570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242020
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38271.5812742748533177No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26721.1040409883480704No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23980.9908272043632758No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.5958185273944303No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13420.5544996281299066No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5810.2400628047268821No Hit
GCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4780.19750433848442278No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.18552185769771093No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.17436575489628955No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.1623832741095777No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.161143707131642No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.161143707131642No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.15825138418312537No Hit
GCTGTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.14544252541112304No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.1367655565655731No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.12808858772002313No Hit
GCTGTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2990.12354350880092554No Hit
GCTGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2930.12106437484505413No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.11527972894802081No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2740.11321378398479465No Hit
GCTGTGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.10701594909511611No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.10164449219072803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA150.004586348358.30544334
TAAGATC303.6154415E-758.30544333
AGACACG750.058.12354755
CTCAATC150.004643452458.12354358
CCTAACG150.004643452458.12354357
CTAACGG150.004643452458.12354358
ATGAATT150.004643452458.12354354
GGTAAGG150.00464727858.11145841
ACGGAGT150.00465110658.09937340
ACATTCC150.004670281458.03904328
CCATCGG202.008732E-457.99086827
ACGCGAT150.004701084557.94277224
CCTACGC150.00472041357.88276715
CGTAGCG202.0335868E-457.8468212
ACGTAGC202.0335868E-457.8468211
GACGTAG258.839628E-657.78701410
GGACGTA258.839628E-657.7870149
AAGTGTA150.004751461657.7870110
TGGGGGC36900.052.12775
GTGGGGG133850.051.63514
Produced by FastQC (version 0.11.5)