FastQCFastQC Report
Fri 17 Jun 2016
SRR1526568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199406
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13310.6674824227957032No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC11280.5656800698073278No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10130.5080087860947013No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6530.3274725936030009No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC6450.32346067821429647No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.266290883925258No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC4960.24873875409967602No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4190.21012406848339568No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.19909130116445845No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3900.19558087519934206No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC3670.18404661845681675No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3540.17752725595017202No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG3400.17050640401993922No Hit
TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG3370.16900193574917505No Hit
TCGGGTGGGGGGGCTGGTAAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC3080.15445874246512142No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2980.14944384822924087No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC2890.14493044341694833No Hit
TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG2830.14192150687542No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2810.1409185280282439No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2800.14041703860465585No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC2800.14041703860465585No Hit
TCGGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAAGGTC2790.13991554918106777No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAAAGGTCG2770.13891257033389165No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2730.13690661263953943No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCAGAGGTCCT2700.13540214436877526No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTCCCGAAGGTCC2580.12938427128571858No Hit
TCGGGTGGGGGGCTGTCAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCTGAAGGTCCA2580.12938427128571858No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2570.12888278186213054No Hit
TCGGGTGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAAGGTCC2540.12737831359136634No Hit
TCGGGTGGGACAACAGTGTCCTGAGGCCAGCTACTGCCACCAGGAGCAGGAGAGATGGCTGCTC2530.1268768241677783No Hit
TCGGGTGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAACACTGACTGGTCTTCCAAAGGTCCT2350.11785001454319328No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCC2310.11584405684884105No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCCAGAGGTCC2210.1108291626129605No Hit
TCGGGTGGGAAGTTTTTAAATCACTGATGACAGCTTTTCTTTCTGCCTTTTCTCTCCCTTTCAT2150.10782022607143216No Hit
TCGGGTGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGAACTGACTGTTCTTCCAGAGGTCCT2040.10230384241196352No Hit
TCGGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2040.10230384241196352No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC2020.10130086356478742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATATC150.00462521558.1775552
CAATTAT201.9786802E-458.16288439
GTCAATT201.9786802E-458.16288440
AACGATA150.004629836458.16288444
AGATGCG150.004629836458.16288442
TCGCCGT150.004634461458.14822451
GCGTTAG150.004634461458.14822446
AATGCGT201.9811487E-458.14822448
CTAGTAA150.004634461458.14822450
ATGCGTG201.9811487E-458.14822449
AGTTAGT150.004634461458.14822449
TTACCTA150.004634461458.14822448
ATCGAAC150.004634461458.14822451
ACGCATA150.004652995658.08965336
CGCATAG150.004652995658.08965337
CCAATTA201.9910472E-458.0896538
AGCAAAC150.004666932458.04579534
CATAAGC201.9984969E-458.04579534
ACATAAG201.9984969E-458.04579533
CGACCCG150.00467158558.03119332
Produced by FastQC (version 0.11.5)