FastQCFastQC Report
Fri 17 Jun 2016
SRR1526567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193049
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10430.5402773389139545No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC9130.4729369227501826No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8280.42890665064310096No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT7600.39368243295743566No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC4690.2429435013908386No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.22481338934674616No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC4130.21393532212029073No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3530.18285513004470366No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.17404907562328736No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3080.1595449859880134No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2840.1471129091577786No Hit
TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG2780.14400488995021987No Hit
TCGGGTGGGGGGGCTGGTAAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC2780.14400488995021987No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG2730.14141487394392097No Hit
TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG2560.13260881952250464No Hit
TCGGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAAGGTC2330.12069474589352962No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCAGAGGTCCT2310.11965873949101005No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC2310.11965873949101005No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2270.11758672668597092No Hit
TCGGGTGGGACAACAGTGTCCTGAGGCCAGCTACTGCCACCAGGAGCAGGAGAGATGGCTGCTC2250.11655072028345136No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2250.11655072028345136No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2220.11499671067967199No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCCAGAGGTCC2160.11188869147211329No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAAAGGTCG2120.10981667866707416No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2090.10826266906329482No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTCCCGAAGGTCC2070.10722666266077524No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.10670865945951546No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC2010.10411864345321654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG258.362082E-658.3232233
CTCATGA150.00457939958.32321533
CATGCGA201.9593471E-458.27746235
TAGTGGA201.9644202E-458.24699838
CGGTAAG150.00463662258.1406231
ACGTCCC150.004641414658.12545832
TACAGCG150.004641414658.12545855
ACGGGAT201.9873791E-458.11030245
TATAGCA201.9873791E-458.11030230
TAGCATA303.696241E-758.110330
CTATTCG150.00464621158.110330
TGAATAG150.00464621158.110345
ATAGGCC150.004655814358.08001340
TCGTACT150.004655814358.08001341
AAACCGC150.004665432558.04975526
TTATTGC150.004665432558.04975526
TAACGGG202.000226E-458.0346443
TATGATC351.6509148E-857.91398222
TGATTAA150.004708898357.91398223
ACCGAGT150.004733175557.8388315
Produced by FastQC (version 0.11.5)