##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526567_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 193049 Sequences flagged as poor quality 0 Sequence length 64 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.968919807924415 38.0 37.0 38.0 33.0 39.0 2 36.72596076643754 38.0 37.0 38.0 33.0 39.0 3 36.71001662790276 38.0 37.0 38.0 33.0 39.0 4 36.550642582971165 38.0 36.0 38.0 33.0 39.0 5 36.646038052515166 38.0 37.0 38.0 33.0 39.0 6 34.158369118721154 36.0 33.0 38.0 27.0 39.0 7 37.68847805479438 38.0 38.0 39.0 35.0 40.0 8 36.550870504379716 38.0 35.0 39.0 31.0 40.0 9 36.698221695010076 38.0 35.0 39.0 31.0 40.0 10 35.90232531637046 38.0 35.0 39.0 31.0 40.0 11 36.03196597754974 38.0 35.0 39.0 31.0 40.0 12 36.22070044392874 38.0 35.0 39.0 31.0 40.0 13 36.18937161031655 38.0 35.0 39.0 31.0 40.0 14 36.25634942423944 38.0 35.0 39.0 31.0 40.0 15 36.227304984744805 38.0 36.0 39.0 31.0 40.0 16 36.69012530497439 38.0 36.0 39.0 33.0 40.0 17 36.24103206957819 38.0 36.0 39.0 31.0 40.0 18 36.53876476956628 38.0 36.0 39.0 31.0 40.0 19 36.43761428445628 38.0 35.0 39.0 31.0 40.0 20 36.31265119218437 38.0 35.0 39.0 31.0 40.0 21 36.30653357437749 38.0 35.0 39.0 31.0 40.0 22 36.230438904112425 38.0 35.0 39.0 31.0 40.0 23 36.10482830783894 38.0 35.0 39.0 31.0 40.0 24 35.97499080544318 38.0 35.0 39.0 30.0 40.0 25 35.963201052582505 38.0 35.0 39.0 30.0 40.0 26 36.30266409046408 38.0 36.0 39.0 31.0 40.0 27 35.74123150081068 38.0 35.0 39.0 29.0 40.0 28 35.67280327792426 38.0 35.0 39.0 29.0 40.0 29 35.58043812710763 38.0 35.0 39.0 29.0 40.0 30 35.50166538029205 38.0 35.0 39.0 29.0 40.0 31 35.14035296738134 38.0 35.0 39.0 28.0 40.0 32 35.410434656486174 38.0 35.0 39.0 29.0 40.0 33 35.30274179094427 38.0 35.0 39.0 28.0 40.0 34 34.549368295096066 38.0 34.0 39.0 26.0 40.0 35 34.651497806256444 38.0 34.0 39.0 26.0 40.0 36 34.02181311480505 38.0 33.0 39.0 24.0 40.0 37 33.76129376479547 38.0 33.0 39.0 23.0 40.0 38 33.68609006003657 38.0 33.0 39.0 23.0 40.0 39 33.18595796922025 38.0 33.0 39.0 19.0 40.0 40 33.377945495703166 38.0 33.0 39.0 22.0 40.0 41 33.00622639847914 38.0 33.0 39.0 17.0 40.0 42 32.69707690793529 37.0 32.0 39.0 14.0 40.0 43 32.74532372610063 37.0 32.0 39.0 17.0 40.0 44 32.56090940642013 37.0 32.0 39.0 15.0 39.0 45 32.31752560230822 37.0 31.0 39.0 12.0 39.0 46 31.946267527933323 36.0 31.0 39.0 4.0 39.0 47 31.67717522494289 36.0 31.0 39.0 4.0 39.0 48 31.599795906738702 36.0 31.0 39.0 4.0 39.0 49 31.389750788659875 36.0 30.0 39.0 4.0 39.0 50 31.025216395837326 36.0 30.0 39.0 4.0 39.0 51 30.648078985128127 35.0 29.0 39.0 4.0 39.0 52 30.500499873089215 35.0 29.0 39.0 4.0 39.0 53 30.295355065294302 35.0 29.0 38.0 4.0 39.0 54 30.092826173665753 35.0 29.0 38.0 4.0 39.0 55 29.91414096939119 35.0 28.0 38.0 4.0 39.0 56 29.443514340918625 35.0 28.0 38.0 4.0 39.0 57 29.068516283430633 35.0 27.0 38.0 4.0 39.0 58 28.72959714891038 34.0 26.0 38.0 4.0 39.0 59 28.403565934037474 34.0 25.0 38.0 4.0 39.0 60 28.22326973980699 34.0 24.0 38.0 4.0 39.0 61 27.764919787204285 34.0 23.0 38.0 4.0 39.0 62 27.352765360089926 33.0 20.0 38.0 4.0 39.0 63 27.17592942724386 33.0 18.0 38.0 4.0 39.0 64 27.162590844811422 33.0 18.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.1150751122069309 2 2 0.3399939757749664 2 3 -0.030800368622458052 2 4 0.37975648346298385 2 5 0.20260032783584592 2 6 0.35763257375072044 2 7 0.3789040880135204 2 8 0.592897616510129 2 9 0.10468957025219794 2 10 0.30813432832371035 2 11 -0.12360679511853334 2 12 -0.21074147875815186 2 13 -0.01191053293914024 2 14 0.20532007449946832 2 15 0.011133450387518451 2 16 0.007312435747813595 2 17 0.6843269923046336 2 18 -0.015379668374528421 2 19 -0.01019977366662772 2 20 0.15142728387286297 2 21 -0.5074453157406609 2 22 -0.46756052519222635 2 23 -0.15015853720475292 2 24 0.19176088150204862 2 25 0.2437748638630879 2 26 0.619333331327951 2 27 0.6811971850341862 2 28 0.6826713316067057 2 29 0.7462271683742614 2 30 0.5223569094806706 2 31 0.7163438397097579 2 32 0.3921619613841969 2 33 -0.03166656962888936 2 34 0.5182338609825194 2 35 0.5878762831869082 2 36 -0.614539776858571 2 37 -0.4920384755522704 2 38 -0.3901162646223284 2 39 -3.715184571236321 2 40 -1.2172955302721888 2 41 -1.4051777485599537 2 42 -0.7952281892177417 2 43 -1.6757374574154973 2 44 -1.5282619410614124 2 45 -1.8097192999975071 2 46 0.2089272294742699 2 47 -0.3575192818302533 2 48 -0.4412201571459029 2 49 -0.13460222631922747 2 50 -0.4413861475446481 2 51 -0.18170883797955284 2 52 0.1452942043381107 2 53 -0.546330086848748 2 54 0.488981467289328 2 55 -0.7248730237733518 2 56 0.4314439250587867 2 57 0.2799850871163123 2 58 -0.013896629861847032 2 59 0.18221962171800854 2 60 -0.0056440039311453916 2 61 -0.8392918165066803 2 62 -0.8556950587205208 2 63 -0.5205115176448878 2 64 -1.2166980371372667 3 1 -0.05457975108307522 3 2 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0.24461883805614804 118 62 0.2834592652164112 118 63 0.14760840590981772 118 64 -0.28699187373545243 119 1 0.04823884948722679 119 2 0.44441118206543706 119 3 0.3056936184265737 119 4 0.6206214233334748 119 5 0.2549823814898744 119 6 0.7851302610772706 119 7 0.4832056606314623 119 8 0.6567274037441706 119 9 0.4771456294566363 119 10 0.429318417130375 119 11 0.11008885705538063 119 12 -0.1493399986471431 119 13 0.01295070665382525 119 14 -0.15337557767444565 119 15 0.05623058267244119 119 16 -0.05998636166199134 119 17 0.021630415061338226 119 18 -0.08348790149201335 119 19 -0.24632373296357457 119 20 0.16102487869247994 119 21 0.40559816252020653 119 22 -0.24346246783792935 119 23 -0.024811636187180852 119 24 -0.04031589925650536 119 25 -0.8984545533431998 119 26 0.36019827119844194 119 27 0.48034149585749475 119 28 0.5413669837806196 119 29 0.6443539028793523 119 30 0.389609453421464 119 31 0.3781153475728516 119 32 -0.1725928952300464 119 33 0.24319466996407613 119 34 0.7706621681055523 119 35 0.6892870139917164 119 36 0.3076387615318126 119 37 0.01802165395744737 119 38 0.03969409615473296 119 39 0.7261891567932821 119 40 0.3449153855464928 119 41 -0.06139884014181973 119 42 -0.19763336960627242 119 43 0.4456778987362142 119 44 0.002149714812780701 119 45 0.2534166852013797 119 46 0.18395035620877564 119 47 -0.1401279774824289 119 48 0.05022295108721764 119 49 -0.11378816526464774 119 50 0.5632391993563672 119 51 0.17062696220546059 119 52 -0.027115601397319722 119 53 -0.3167972468857485 119 54 -0.16643018858486158 119 55 0.07450255439501063 119 56 0.007762148925575474 119 57 0.3727232924817159 119 58 0.07676016939809571 119 59 0.39278853938683156 119 60 0.4514559035619179 119 61 -0.09218265831981398 119 62 0.013176356635629105 119 63 -0.021552220697618196 119 64 -0.14974614074503734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 120.0 5 15.0 6 49.0 7 60.0 8 147.0 9 134.0 10 159.0 11 261.0 12 349.0 13 502.0 14 567.0 15 749.0 16 996.0 17 1035.0 18 1183.0 19 1530.0 20 1706.0 21 2368.0 22 2967.0 23 2310.0 24 3051.0 25 2497.0 26 2779.0 27 3419.0 28 3966.0 29 4675.0 30 5640.0 31 6593.0 32 8264.0 33 10584.0 34 13568.0 35 18067.0 36 23698.0 37 30894.0 38 27387.0 39 10757.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.06966143135386 0.5035017197795367 0.18907214785959967 0.23776470100700342 8 98.49014160429581 0.7743660978997782 0.4654489664752348 0.27004333132917296 9 96.83966246911406 1.7990251179752292 0.5190392076623034 0.8422732052484083 10 55.37350620826837 29.283756973618097 6.322229071375661 9.020507746737875 11 40.52339043455289 20.599951307699083 19.951411299721833 18.9252469580262 12 41.32266937409673 14.635662448393932 23.658760211138105 20.382907966371235 13 29.933850991199122 14.010432584473373 28.140523908437753 27.915192515889746 14 23.187377297991702 18.821646317774242 34.74920875011008 23.241767634123978 15 30.233582847782365 19.868019988802953 31.254276650009334 18.644120513405355 16 37.39099797410376 20.122693664799666 23.650654659820418 18.835653701276158 17 33.855633528871735 21.937645551630958 23.175709669555655 21.03101124994165 18 33.12319670135561 21.988199887075304 24.626390191091378 20.262213220477705 19 29.914684872752513 26.82790379644546 22.695792259996168 20.561619070805857 20 30.448322676256307 26.37393386048729 23.090275978568396 20.08746748468801 21 28.19232422856373 24.08922087138499 27.977352900040923 19.74110200001036 22 26.15967966682034 21.233987226041055 31.930235328854334 20.67609777828427 23 29.66656133934908 21.842122984320042 29.145450118881733 19.345865557449144 24 31.87664796597614 23.902176221631674 23.47998611693889 20.741189695453297 25 30.292209138402853 20.983808773530143 21.76019985073389 26.963782237333113 26 23.16038175770199 20.054196329571713 28.568097739919796 28.217324172806503 27 21.505649820150417 19.046926985741795 30.256772258007587 29.190650936100198 28 21.83309383867571 24.357903583082166 25.46027068948523 28.348731888756895 29 27.255376859924173 24.55903784417349 25.20213466654911 22.98345062935322 30 27.380581677772938 19.412094413651367 30.59569035311702 22.61163355545868 31 28.765665396476 18.814050316163264 30.991787023289135 21.428497264071602 32 33.64187762344798 18.597749217829847 29.49365183959156 18.26672131913061 33 32.616076249013666 18.868619959300638 30.47572573611861 18.03957805556709 34 23.34042099681315 21.72568142945522 37.620416629842005 17.31348094388962 35 21.312701215079446 29.248623948488937 33.32277495066985 16.11589988576176 36 23.94345702109968 31.047708138447145 28.155077434778093 16.853757405675086 37 26.46795858111199 29.280159687279077 26.95502557491579 17.296856156693146 38 27.259546427754795 27.21797243644376 26.640613632111748 18.88186750368969 39 26.918725004041065 23.85689927573639 27.053774878637615 22.170600841584935 40 25.931008670700518 21.821262969662943 28.038383964402303 24.209344395234233 41 20.112856251300187 24.320262117744956 28.919284376950284 26.64759725400458 42 18.783779561998188 23.67927770314599 31.19226884105512 26.344673893800703 43 21.321247839396907 19.776026156313126 32.025344134613384 26.877381869676586 44 24.110780951587973 19.193601265592232 30.260247810452583 26.435369972367205 45 24.3668695113508 22.103997376380132 28.040978443631214 25.488154668637854 46 22.61901042480291 23.70731402505702 28.577602816050657 25.096072734089418 47 19.570526271941016 22.60308567084442 33.60444469900911 24.221943358205458 48 23.52815610812612 22.70450664334208 32.19105335499188 21.576283893539923 49 26.832811743325646 20.96010747704917 30.138876594060644 22.06820418556454 50 21.200700591123763 21.436316058340893 30.941732086447942 26.42125126408741 51 19.261624696554527 23.247830299746823 33.64988904053927 23.840655963159374 52 19.22748301587963 23.183845288209064 33.463405993414746 24.125265702496563 53 21.302129743982572 20.500674827124403 35.32915409508028 22.868041333812748 54 20.238330953844873 20.69529694973895 33.50962390187476 25.556748194541417 55 21.929824561403507 20.47930283224401 30.279575945210617 27.311296661141863 56 20.31808751316242 24.751608161222723 26.79504154633694 28.13526277927792 57 23.66094697996372 27.731272022183767 26.42974793069658 22.178033067155933 58 23.960050067800147 31.225618024408053 25.325440700949205 19.4888912068426 59 26.33580022843553 29.516686746045405 25.35842994904532 18.789083076473748 60 29.910155271725518 25.190437432172967 25.09808832122882 19.801318974872693 61 27.562605558914903 21.807168623198983 27.695531599908257 22.934694217977857 62 22.362013410221383 21.2248558349062 29.561925816240343 26.851204938632073 63 20.54799520308671 22.556963345325617 26.8877418009281 30.007299650659576 64 22.048271363339854 22.82948467058056 26.57638617090672 28.545857795172864 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 2.0 6 4.0 7 7.0 8 10.0 9 6.5 10 42.0 11 81.0 12 130.0 13 218.0 14 257.0 15 289.0 16 489.0 17 657.0 18 904.5 19 1152.0 20 1020.0 21 1028.5 22 1169.0 23 1384.5 24 1963.0 25 2326.0 26 2496.0 27 2910.5 28 3155.0 29 3285.0 30 3050.5 31 2686.0 32 2913.5 33 3141.0 34 2922.0 35 2683.0 36 2663.0 37 2776.5 38 3162.0 39 3434.0 40 3806.0 41 4004.0 42 3830.0 43 4055.5 44 4281.0 45 4412.0 46 4727.5 47 4912.0 48 5023.0 49 5340.5 50 5547.0 51 6000.0 52 6681.0 53 6909.0 54 7029.0 55 8239.0 56 9329.0 57 9682.5 58 10036.0 59 10700.5 60 12515.5 61 13666.0 62 15091.5 63 15481.5 64 14446.0 65 11641.0 66 7734.0 67 6632.0 68 5627.5 69 4623.0 70 3754.5 71 2417.0 72 1948.0 73 1708.0 74 1262.0 75 1056.0 76 891.5 77 607.0 78 487.0 79 398.5 80 237.5 81 165.0 82 121.0 83 77.0 84 63.0 85 37.5 86 26.0 87 23.5 88 14.5 89 8.0 90 9.5 91 16.0 92 21.0 93 10.5 94 0.0 95 0.0 96 1.5 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 5.180032012597837E-4 8 0.060606374547394704 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.07303845137762952 16 0.02486415366046962 17 0.12846479391242638 18 0.0 19 0.0 20 0.03263420167936638 21 0.0 22 0.0 23 0.0 24 0.00414402561007827 25 0.0543903361322773 26 0.024346150459209837 27 0.20098524208879612 28 0.14607690275525903 29 0.12017674269226984 30 0.18907116845982108 31 0.22118736693792768 32 0.16368901159809168 33 0.21600733492532984 34 0.3605302280768095 35 0.2429435013908386 36 0.32582401359240404 37 0.3486161544478345 38 0.3216799879823257 39 0.6568280591974058 40 0.3506881672528736 41 0.3983444617687737 42 0.5154131852534849 43 0.5040171148257696 44 0.45895083631616845 45 0.491067034794275 46 0.5216292236686022 47 0.5180032012597838 48 0.5060891276308087 49 0.522147226869862 50 0.6273018767255982 51 0.5630694797693849 52 0.5723935373920611 53 0.5946676750462317 54 0.5858616206248155 55 0.6148697998953633 56 0.6293738895306373 57 0.6200498319079613 58 0.6780661904490569 59 0.6785841936503167 60 0.7174344337448005 61 0.6283378831281178 62 0.6500940175810287 63 0.6521660303860678 64 0.7376365585939321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 193049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.283078389424446 #Duplication Level Percentage of deduplicated Percentage of total 1 77.77277116189249 37.551088065724244 2 9.02478274863212 8.714885857994602 3 4.170153417015341 6.040435329890339 4 2.4299967814612167 4.693109003413641 5 1.632872009441047 3.9420043615869544 6 1.1125415727926187 3.223015918238375 7 0.7434824589636305 2.5128335293112114 8 0.5610985945713979 2.1673253940709354 9 0.4023173479240425 1.7482608042517702 >10 1.926831884990881 16.254422452330754 >50 0.1437613989915245 4.753197374759776 >100 0.0750992382791546 6.563618563162721 >500 0.00321853878339234 1.2955260063507192 >1k 0.0010728462611307799 0.5402773389139545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 1043 0.5402773389139545 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 913 0.4729369227501826 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 828 0.42890665064310096 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 760 0.39368243295743566 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC 469 0.2429435013908386 No Hit TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.22481338934674616 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC 413 0.21393532212029073 No Hit TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 353 0.18285513004470366 No Hit TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 336 0.17404907562328736 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 308 0.1595449859880134 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 284 0.1471129091577786 No Hit TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG 278 0.14400488995021987 No Hit TCGGGTGGGGGGGCTGGTAAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC 278 0.14400488995021987 No Hit TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG 273 0.14141487394392097 No Hit TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG 256 0.13260881952250464 No Hit TCGGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAAGGTC 233 0.12069474589352962 No Hit TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCAGAGGTCCT 231 0.11965873949101005 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC 231 0.11965873949101005 No Hit TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 227 0.11758672668597092 No Hit TCGGGTGGGACAACAGTGTCCTGAGGCCAGCTACTGCCACCAGGAGCAGGAGAGATGGCTGCTC 225 0.11655072028345136 No Hit TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 225 0.11655072028345136 No Hit TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 222 0.11499671067967199 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCCAGAGGTCC 216 0.11188869147211329 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAAAGGTCG 212 0.10981667866707416 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 209 0.10826266906329482 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTCCCGAAGGTCC 207 0.10722666266077524 No Hit TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 206 0.10670865945951546 No Hit TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTGTGGTTGTC 201 0.10411864345321654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.180032012597837E-4 0.0 0.0 0.0 0.0 8 0.0010360064025195675 0.0 0.0 0.0 0.0 9 0.0010360064025195675 0.0 0.0 0.0 0.0 10 0.0015540096037793515 0.0 0.0 0.0 0.0 11 0.0036260224088184865 0.0 0.0 0.0 0.0 12 0.00414402561007827 0.0 0.0 0.0 0.0 13 0.004662028811338054 0.0 0.0 0.0 0.0 14 0.007252044817636973 0.0 0.0 0.0 0.0 15 0.008806054421416324 0.0 0.0 0.0 0.0 16 0.010360064025195676 0.0 0.0 0.0 0.0 17 0.012432076830234812 0.0 0.0 0.0 0.0 18 0.013468083232754379 0.0 0.0 0.0 0.0 19 0.014504089635273946 0.0 0.0 0.0 0.0 20 0.017094105641572866 0.0 0.0 0.0 0.0 21 0.01761210884283265 0.0 0.0 0.0 0.0 22 0.021238131251651134 0.0 0.0 0.0 0.0 23 0.023310144056690272 0.0 0.0 0.0 0.0 24 0.02590016006298919 0.0 0.0 0.0 0.0 25 0.02745416966676854 0.0 0.0 0.0 0.0 26 0.03004418567306746 0.0 0.0 0.0 0.0 27 0.031080192075587026 0.0 0.0 0.0 0.0 28 0.037296230490704435 0.0 0.0 0.0 0.0 29 0.04040424969826314 0.0 0.0 0.0 0.0 30 0.041958259302042486 0.0 0.0 0.0 0.0 31 0.044030272107081624 0.0 0.0 0.0 0.0 32 0.044548275308341406 0.0 0.0 0.0 0.0 33 0.04558428171086097 0.0 0.0 0.0 0.0 34 0.05076431372345881 0.0 0.0 0.0 0.0 35 0.06216038415117405 0.0 0.0 0.0 0.0 36 0.06423239695621319 0.0 0.0 0.0 0.0 37 0.06578640655999254 0.0 0.0 0.0 0.0 38 0.06682241296251211 0.0 0.0 0.0 0.0 39 0.0699304321700708 0.0 0.0 0.0 0.0 40 0.07355645457888929 0.0 0.0 0.0 0.0 41 0.07718247698770779 0.0 0.0 0.0 0.0 42 0.08132650259778605 0.0 0.0 0.0 0.0 43 0.08236250900030562 0.0 0.0 0.0 0.0 44 0.0833985154028252 0.0 0.0 0.0 0.0 45 0.08754254101290346 0.0 0.0 0.0 0.0 46 0.08909655061668281 0.0 0.0 0.0 0.0 47 0.09013255701920238 0.0 0.0 0.0 0.0 48 0.09479458583054043 0.0 0.0 0.0 0.0 49 0.0994566146418785 0.0 0.0 0.0 0.0 50 0.10360064025195675 0.0 0.0 0.0 0.0 51 0.10619065625825568 0.0 0.0 0.0 0.0 52 0.11240669467337308 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCGCG 25 8.362082E-6 58.32322 33 CTCATGA 15 0.004579399 58.323215 33 CATGCGA 20 1.9593471E-4 58.277462 35 TAGTGGA 20 1.9644202E-4 58.246998 38 CGGTAAG 15 0.004636622 58.14062 31 ACGTCCC 15 0.0046414146 58.125458 32 TACAGCG 15 0.0046414146 58.125458 55 ACGGGAT 20 1.9873791E-4 58.110302 45 TATAGCA 20 1.9873791E-4 58.110302 30 TAGCATA 30 3.696241E-7 58.1103 30 CTATTCG 15 0.004646211 58.1103 30 TGAATAG 15 0.004646211 58.1103 45 ATAGGCC 15 0.0046558143 58.080013 40 TCGTACT 15 0.0046558143 58.080013 41 AAACCGC 15 0.0046654325 58.049755 26 TTATTGC 15 0.0046654325 58.049755 26 TAACGGG 20 2.000226E-4 58.03464 43 TATGATC 35 1.6509148E-8 57.913982 22 TGATTAA 15 0.0047088983 57.913982 23 ACCGAGT 15 0.0047331755 57.83883 15 >>END_MODULE