FastQCFastQC Report
Fri 17 Jun 2016
SRR1526566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176463
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC18471.0466783405019748No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC14740.8353025846778078No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC8810.4992548012897888No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5710.32358058063163386No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5230.29637941098133885No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC4760.269744932365425No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4340.24594390892141693No Hit
TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA3560.20174200823968763No Hit
TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3420.1938083337583516No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.19210826065520817No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC3310.18757473238015904No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3310.18757473238015904No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3160.17907436686444184No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.16490709100491321No Hit
TCGGGTGGGACAACAGTGTCCTGAGGCCAGCTACTGCCACCAGGAGCAGGAGAGAT2560.14507290480157314No Hit
TCGGGTGGGGGGGCTGGTAAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC2490.1411060675609051No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCC2390.13543915721709365No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTG2380.1348724661827125No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC2300.13033893790766338No Hit
TCGGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTC2290.1297722468732822No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2290.1297722468732822No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC2280.12920555583890106No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTCCC2260.1280721737701388No Hit
TCGGGTGGGGGGCTGTCAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCTG2210.12523871859823307No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC2200.12467202756385191No Hit
TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA2150.12183857239194619No Hit
TCGGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2120.12013849928880274No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2100.11900511722004047No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCC2080.11787173515127816No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA2060.11673835308251589No Hit
TCGGGTGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT2040.1156049710137536No Hit
TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1970.11163813377308557No Hit
TCGGGTGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAACACTGACTGGTCTTCCA1970.11163813377308557No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCC1920.10880467860117984No Hit
TCGGGTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCT1800.1020043861886061No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGCCTGCTCTTC1780.10087100411984382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT150.00803025350.6016737
AGTAATC150.00803025350.6016737
TAGAGTG150.00805772750.55798336
CTAGAGT150.00807608150.52890435
TTGGTTG150.00807608150.52890434
ACATACT309.697251E-750.52890434
ACGTCCC251.9779909E-550.4273832
AATACTC150.00814056550.4273832
TTTCGCA150.00815905950.39845331
GCCTAAG150.00815905950.39845331
TACTAAC150.00815905950.39845331
GCAATTA150.00815905950.39845331
GCACTTC150.00828939550.19685748
CGTTAGT150.0083081450.16818647
TCCGAGT150.00832691650.13955340
AGTCTGC204.1171093E-450.13955338
GTCGTAC204.1171093E-450.13955340
CCGCACG150.00832691650.13955339
GTACGTA301.0229032E-650.13955339
TACGTAG301.0229032E-650.13955340
Produced by FastQC (version 0.11.5)