##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526566_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176463 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.15868482344741 38.0 37.0 38.0 34.0 39.0 2 36.867178955361744 38.0 37.0 38.0 33.0 39.0 3 36.85987430792857 38.0 37.0 38.0 33.0 39.0 4 36.7512906388308 38.0 37.0 38.0 33.0 39.0 5 36.67424899270669 38.0 37.0 38.0 33.0 39.0 6 34.26068354272567 36.0 33.0 37.0 28.0 39.0 7 37.80423658217303 38.0 38.0 39.0 36.0 40.0 8 37.08045312615109 38.0 36.0 39.0 31.0 40.0 9 37.04777205419833 38.0 35.0 39.0 31.0 40.0 10 36.22485733553209 38.0 35.0 39.0 31.0 40.0 11 36.66152111207449 38.0 35.0 39.0 32.0 40.0 12 36.84056714438721 38.0 35.0 39.0 32.0 40.0 13 36.8660342394723 38.0 36.0 39.0 32.0 40.0 14 36.943670911182515 38.0 36.0 39.0 33.0 40.0 15 37.09234230405241 38.0 37.0 39.0 33.0 40.0 16 37.59500858536917 39.0 38.0 39.0 35.0 40.0 17 37.39961351671455 39.0 37.0 39.0 33.0 40.0 18 37.35163745374384 39.0 37.0 39.0 33.0 40.0 19 37.21501957917524 39.0 37.0 39.0 33.0 40.0 20 37.161982965267505 39.0 37.0 39.0 33.0 40.0 21 37.00251044128231 38.0 36.0 39.0 33.0 40.0 22 36.948504785705786 38.0 36.0 39.0 33.0 40.0 23 36.826462204541464 38.0 36.0 39.0 33.0 40.0 24 36.68915296691091 38.0 36.0 39.0 32.0 40.0 25 36.65035163178683 38.0 36.0 39.0 32.0 40.0 26 36.20584485132861 38.0 35.0 39.0 31.0 40.0 27 36.00639794177816 38.0 35.0 39.0 30.0 40.0 28 35.85518777307424 38.0 35.0 39.0 30.0 40.0 29 35.71945393651927 38.0 35.0 39.0 30.0 40.0 30 35.42793673461292 38.0 35.0 39.0 29.0 40.0 31 34.71127658489315 38.0 33.0 39.0 27.0 40.0 32 34.71154292967931 38.0 34.0 39.0 27.0 40.0 33 34.53654873826241 38.0 33.0 39.0 27.0 40.0 34 34.55259176144574 38.0 33.0 39.0 27.0 40.0 35 34.40618713271337 38.0 33.0 39.0 26.0 40.0 36 33.71961827691924 38.0 33.0 39.0 23.0 40.0 37 33.23670684506101 38.0 33.0 39.0 18.0 40.0 38 33.20067662909505 37.0 33.0 39.0 20.0 40.0 39 33.09802054821691 37.0 33.0 39.0 19.0 40.0 40 32.77509166227481 37.0 32.0 39.0 16.0 40.0 41 32.46690241013697 37.0 32.0 39.0 11.0 40.0 42 32.29077483665131 37.0 32.0 39.0 10.0 40.0 43 32.2269144239869 36.0 32.0 39.0 8.0 40.0 44 31.778582479046598 36.0 31.0 39.0 4.0 40.0 45 31.58785694451528 36.0 31.0 39.0 4.0 40.0 46 30.898919320197436 36.0 30.0 38.0 4.0 39.0 47 30.608184151918532 35.0 29.0 38.0 4.0 39.0 48 30.313074128854208 35.0 29.0 38.0 4.0 39.0 49 30.187954415373195 35.0 29.0 38.0 4.0 39.0 50 29.814856372157337 35.0 28.0 38.0 4.0 39.0 51 29.129177221287183 35.0 28.0 38.0 4.0 39.0 52 28.83007202643047 35.0 27.0 38.0 4.0 39.0 53 28.557295297031107 35.0 26.0 38.0 4.0 39.0 54 28.299841893201407 34.0 25.0 38.0 4.0 39.0 55 27.885942095510106 34.0 23.0 38.0 4.0 39.0 56 16.85819123555646 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3877607848400402 1 2 -1.1222766702082723 1 3 -1.9039438677874045 1 4 -2.575559786671434 1 5 -2.842900645332456 1 6 -0.3891615649228939 1 7 -1.5774747298156413 1 8 -2.803523611935219 1 9 -2.7685621701919416 1 10 -1.0372388903392604 1 11 -1.4270883735527988 1 12 -1.2018529949556012 1 13 -1.601554476586493 1 14 -1.5206930335902058 1 15 -1.4442652405498606 1 16 -1.1733546283720315 1 17 -1.2269876211561836 1 18 -1.4296468375803073 1 19 -1.353481315998664 1 20 -1.6987589829025964 1 21 -1.5367738934881743 1 22 -1.3099482939216784 1 23 -1.2741904771107642 1 24 -1.660315427286065 1 25 -1.2702449823612696 1 26 -1.068268750499442 1 27 -1.5746806283703663 1 28 -1.1563059703749232 1 29 -1.0134749708238076 1 30 -1.366850074637341 1 31 -0.9809030052477752 1 32 -0.9187626120610233 1 33 -1.2976722294438332 1 34 -1.066971982835888 1 35 -0.7277926048916683 1 36 -0.5301978018017124 1 37 -0.3076228560872849 1 38 -0.9229396278309068 1 39 -0.28716290234437736 1 40 -0.9230499423746679 1 41 -0.7240142689361484 1 42 -0.8144228025308884 1 43 -0.6256436455685588 1 44 -0.5640275237746266 1 45 0.0811267189971474 1 46 0.4959200251460487 1 47 0.48381559852867184 1 48 0.47665574239895037 1 49 0.03881946029177641 1 50 0.319503523793891 1 51 0.6093270334944023 1 52 1.2379633435281683 1 53 0.8731168418581952 1 54 0.631521284170887 1 55 1.6081815806931203 1 56 1.793367140012844 2 1 -0.1371384340360251 2 2 0.30711174397459473 2 3 -0.1726984933677045 2 4 0.14421414860408532 2 5 0.45542405358431637 2 6 -0.5651354598297118 2 7 0.3693731474635058 2 8 0.2975999835704002 2 9 -0.003407087099695616 2 10 0.5468962102460821 2 11 -0.11537472220381062 2 12 -0.27670329435679974 2 13 -0.0722198873380222 2 14 -0.07623363777509695 2 15 -0.14382118484105177 2 16 -0.013124526777467338 2 17 0.3023469680922872 2 18 0.40285669468101304 2 19 0.2853533741715566 2 20 0.40646876385108754 2 21 0.5448045227131288 2 22 0.724600585846872 2 23 0.608975774655363 2 24 0.3359168273058799 2 25 0.35250950865669495 2 26 0.5939797861351508 2 27 -0.16275167728377227 2 28 0.8446023253930903 2 29 0.5907771989938553 2 30 0.04480975177724389 2 31 0.885913922694094 2 32 0.4260197333604907 2 33 1.1774000978117698 2 34 0.7809389368976767 2 35 1.3861480531181556 2 36 1.6418065041930348 2 37 1.9443114774927466 2 38 2.2235651154914393 2 39 2.185588224615188 2 40 2.1160740568179577 2 41 2.3008461226402517 2 42 1.8787750713842044 2 43 2.284872400990011 2 44 2.469444857305909 2 45 1.2695812705681604 2 46 1.5654551122751599 2 47 1.8134260405659468 2 48 1.821438087820468 2 49 1.8520805785047862 2 50 1.6225715450547398 2 51 2.1319671465267653 2 52 2.3240832385695462 2 53 2.7505305537602354 2 54 1.7183139908922733 2 55 1.6205613156053182 2 56 0.43354745354308477 3 1 0.05452227908990892 3 2 0.6730921297640364 3 3 0.09446394087272836 3 4 0.2834620540402071 3 5 0.6855338152262007 3 6 0.08223091024238016 3 7 0.5771408944272025 3 8 0.39878852679310484 3 9 0.11067357796492416 3 10 0.11350009411956563 3 11 -0.09082448514464403 3 12 -0.40676109782500447 3 13 -0.14473401346648984 3 14 -0.0531771982089424 3 15 -0.3896807678767047 3 16 -0.025626962316863455 3 17 0.05064209051872837 3 18 -0.5068335047084815 3 19 -0.21513373370660105 3 20 -0.13155812450379045 3 21 -0.20407512916710857 3 22 -0.3142056751130511 3 23 -0.7606741571495306 3 24 0.2944801838038984 3 25 -0.17350205203694458 3 26 -0.27411140045963833 3 27 -0.5085008492003809 3 28 -0.10009469350669775 3 29 0.16536965206913123 3 30 -0.09167787568264885 3 31 0.5092496374437161 3 32 0.632066365821359 3 33 0.02412348548468657 3 34 0.2638576873036058 3 35 0.0024694144223076364 3 36 0.9914525391349116 3 37 1.6114869012765993 3 38 1.6464072275911974 3 39 1.4369034158862135 3 40 1.6007463955852543 3 41 1.1885190459369923 3 42 1.0901149427877215 3 43 0.9047983605925296 3 44 1.5509217683924845 3 45 1.4075951693796185 3 46 1.2128214823472518 3 47 1.3613899296352443 3 48 1.0621668012061924 3 49 1.1531457210650267 3 50 1.0040419613696088 3 51 0.9367992566780927 3 52 0.9210826540400916 3 53 1.0980657878967541 3 54 1.0753965394406926 3 55 1.0060909642381688 3 56 0.6844404846530416 4 1 -0.21410809022926003 4 2 0.29089698248633766 4 3 -0.01172829892513505 4 4 0.10707494386748806 4 5 0.4445245043681467 4 6 -0.8202992894737946 4 7 0.36264503066339415 4 8 0.1425841724959156 4 9 0.24341387771224987 4 10 0.2561742833134346 4 11 -0.1216991519959123 4 12 0.12748832240966834 4 13 -0.18838082389188315 4 14 0.012241098146319018 4 15 0.106632963042955 4 16 0.030238185999223788 4 17 0.19217405549051847 4 18 0.1272393899646005 4 19 0.29322527082768346 4 20 0.3072290410494958 4 21 0.373405747230386 4 22 -0.09437943164623164 4 23 0.037186768398214554 4 24 0.4714210996600485 4 25 0.09502447871657438 4 26 0.4347252614766006 4 27 -0.07168661639431662 4 28 0.44249642483465124 4 29 0.513471136960618 4 30 -0.01954468541578791 4 31 0.2765820246923383 4 32 0.3147703220707143 4 33 0.5166990280411952 4 34 0.7414112506970483 4 35 0.020710389120310424 4 36 0.7153111802342167 4 37 0.518724449301935 4 38 0.8914316296541216 4 39 1.18589098987119 4 40 0.951201554631318 4 41 1.1322731562135502 4 42 1.5448586345948598 4 43 0.8474102466470086 4 44 1.1964515181415365 4 45 0.7457973776797786 4 46 1.0408302047867686 4 47 0.280222784157413 4 48 1.0814461615606277 4 49 0.7873224543037516 4 50 1.295551427985508 4 51 1.4596264346920087 4 52 0.9445501698754732 4 53 1.6695240274869363 4 54 1.4937967332726814 4 55 1.5962055327889288 4 56 0.9430677388152375 5 1 -0.2310991081933338 5 2 0.26919039565999014 5 3 -0.1790187180868088 5 4 -0.07376337948580414 5 5 0.4248763007753311 5 6 -0.9390118643240939 5 7 0.4993591025196835 5 8 0.25272638806477943 5 9 0.11190190166434633 5 10 0.16115182822360907 5 11 0.23518707554899265 5 12 0.16704670564320168 5 13 0.14926887670691968 5 14 0.03851355323614314 5 15 -0.05582961180734003 5 16 -0.14929025711455068 5 17 -0.15116426786277515 5 18 0.05938759355741752 5 19 0.07095730675582246 5 20 -0.06530089907026593 5 21 0.08729047776930798 5 22 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0.9066756012835384 7 31 0.8881951620539823 7 32 1.0725638259719972 7 33 0.6868314903892241 7 34 0.7567986577012178 7 35 0.3462774368430459 7 36 -0.08018872903814867 7 37 0.6842842388138237 7 38 -0.10374166012994124 7 39 -0.07355375699870592 7 40 -0.22328583422732606 7 41 -0.07930369009383043 7 42 -0.15479660038971588 7 43 -0.22517186186324523 7 44 0.589737673104338 7 45 -0.1299420525506676 7 46 0.49385143505350726 7 47 -6.699757772601345E-4 7 48 0.6740427864925813 7 49 0.6837114944053653 7 50 1.0661193829846027 7 51 0.8181094686241437 7 52 0.47396406934925395 7 53 0.9015690283942135 7 54 0.5571972050563865 7 55 -0.2763402846670715 7 56 0.401133425623442 8 1 -0.22176366515535761 8 2 0.3720384969258106 8 3 -0.1608225155551608 8 4 -0.13199122758707205 8 5 0.108816174193052 8 6 -0.4893283200202916 8 7 0.1970647987222165 8 8 0.13951436274832218 8 9 0.04054114217068161 8 10 -0.04114247557385653 8 11 -0.058483810526951174 8 12 -0.30201975005299886 8 13 -0.35816783215383907 8 14 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1525.0 23 1861.0 24 3518.0 25 3702.0 26 2450.0 27 2996.0 28 3348.0 29 4050.0 30 4798.0 31 5930.0 32 7149.0 33 9167.0 34 12127.0 35 16974.0 36 24471.0 37 32334.0 38 25045.0 39 8014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 98.96742951756674 0.5511578851867813 0.2568667921703826 0.22454580507609606 8 98.75611317953339 0.7434986371080623 0.30091293925638807 0.19947524410216305 9 96.9432685605481 1.7629758079597422 0.4975547281866453 0.7962009033055087 10 55.353813547315866 29.26959192578614 6.400775233335033 8.975819293562957 11 39.994219751449315 20.52838272045698 20.105631208808646 19.371766319285065 12 40.18519463003576 14.635929344961834 24.301978318401023 20.876897706601383 13 29.73824541121935 14.19334364711016 28.276182542515993 27.792228399154496 14 23.149328754469774 18.700237443543404 34.71322600205142 23.437207799935397 15 29.916186396015025 20.038761666751668 31.357848387480665 18.68720354975264 16 36.59010670792177 20.209335668100394 23.98746479431949 19.213092829658343 17 33.62347914293648 21.712200291279192 23.2689005627242 21.39542000306013 18 32.86071301065946 21.778503142301787 24.817100468653486 20.54368337838527 19 30.0312246759944 26.46277123249633 23.051857896556218 20.454146194953047 20 30.526512640043524 26.12048984773012 23.302335333752687 20.050662178473676 21 28.073873843241927 23.64575010058766 27.939568068093596 20.34080798807682 22 26.088755149804776 21.263380992049324 31.62589324674294 21.021970611402956 23 29.75258129228956 21.901032516915823 28.84587049903095 19.500515691763663 24 31.22184253922919 23.925128780537563 23.612882020593553 21.2401466596397 25 30.026305047847973 21.66424781169214 21.722640482561566 26.586806657898315 26 23.56963446371569 20.450073991756014 27.897443457257715 28.082848087270584 27 21.53757133618489 18.953584678746072 30.24484053597159 29.264003449097448 28 22.09667080759435 24.443952827459455 25.221085830653074 28.23829053429312 29 27.375446203088377 24.022609514837495 25.273964439954828 23.3279798421193 30 27.582426944594324 19.08897282253713 30.5284979978985 22.80010223497004 31 28.944020356234095 18.460900581606687 31.00122682660851 21.59385223555071 32 33.17800029551164 18.38196015139288 29.518770671607015 18.92126888148847 33 32.726177075871796 18.9349751566213 29.834225875771736 18.50462189173517 34 23.31605039341429 21.699185882566972 37.467025969891296 17.517737754127438 35 21.592007551203757 28.579145485767572 33.2815893919234 16.547257571105273 36 24.300803713120487 30.46032910715606 28.172050350095844 17.066816829627605 37 26.797768287642924 28.469256555081046 27.11633833861413 17.616636818661892 38 27.229410860568247 26.953883287886466 26.688033336559208 19.128672514986082 39 27.10587887033695 23.518327707029936 27.207209258637278 22.168584163995835 40 26.04491151199808 21.64789648444186 28.062848731729023 24.244343271831035 41 20.662230527323274 24.115240873250993 28.604553119730188 26.61797547969555 42 19.100400050148735 23.491263862136563 30.696155643442484 26.712180444272214 43 21.74974196381221 19.662642632710433 31.63381100916385 26.953804394313508 44 24.220470284886307 19.139978778993484 29.758468436605096 26.881082499515113 45 24.492124434505303 22.243266833629036 27.803798298809397 25.46081043305627 46 22.60306807286673 23.512760809021596 28.230151120851026 25.65401999726065 47 20.324089923542164 22.301723154170947 32.386169120164325 24.98801780212256 48 23.727215587680703 22.65470544540312 31.52562710702246 22.092451859893718 49 27.234504427306483 20.84775778349043 29.608111968009137 22.309625821193947 50 21.51135889775564 21.22060310406087 30.58933743095264 26.67870056723085 51 19.794855393612647 23.196989080275962 33.11155023529949 23.896605290811898 52 19.561043621361556 23.146063563534963 32.87258722558084 24.420305589522645 53 21.78311187312473 20.485212173167593 34.69638519788541 23.03529075582226 54 20.92615140134561 20.607754703067926 32.88460549105688 25.58148840452958 55 22.495712815822568 20.581913799016803 29.536984108837316 27.385389276323313 56 21.136157051960005 24.38044168005351 26.46135728291877 28.02204398506771 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 4.5 9 7.0 10 5.0 11 3.0 12 34.5 13 141.5 14 217.0 15 229.5 16 242.0 17 242.5 18 243.0 19 447.5 20 652.0 21 926.0 22 1007.5 23 815.0 24 1004.5 25 1194.0 26 1513.5 27 1833.0 28 2225.0 29 3021.0 30 3425.0 31 3775.0 32 4125.0 33 4095.0 34 4065.0 35 3740.0 36 3415.0 37 3425.5 38 3530.0 39 3624.0 40 3813.5 41 4003.0 42 4189.5 43 4376.0 44 4372.5 45 4369.0 46 4614.5 47 5104.0 48 5348.0 49 5387.5 50 5427.0 51 5841.5 52 6256.0 53 6729.5 54 7955.0 55 8707.0 56 9082.0 57 9457.0 58 10464.0 59 11471.0 60 12759.5 61 14048.0 62 14712.5 63 15699.0 64 16021.0 65 12732.0 66 9443.0 67 7666.5 68 5890.0 69 5273.5 70 4657.0 71 3662.5 72 2251.0 73 1834.0 74 1567.0 75 1300.0 76 1190.0 77 1080.0 78 858.5 79 496.0 80 355.0 81 278.5 82 202.0 83 157.0 84 112.0 85 97.0 86 82.0 87 57.0 88 23.0 89 14.0 90 29.0 91 44.0 92 25.0 93 6.0 94 3.5 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06063594067878252 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 5.666910343811451E-4 24 0.0 25 0.0402350634410613 26 0.05213557516306535 27 0.10483784136051184 28 0.09860423998231925 29 0.14393952273281083 30 0.22554303168369574 31 0.22610972271807686 32 0.2816454440874291 33 0.3179136702878224 34 0.32131381649410923 35 0.3383145475255436 36 0.37061593648526886 37 1.273354754254433 38 0.45335282750491607 39 0.45335282750491607 40 0.6715288757416569 41 0.5298561171463706 42 0.5581906688654279 43 0.6233601378192596 44 0.660195055054034 45 0.6652952743634643 46 0.7021301915982386 47 0.6817293143605175 48 0.8234020729558037 49 0.8007344315805579 50 0.7945008302023654 51 0.7746666439990253 52 0.8256688370933284 53 0.8432362591591438 54 0.8636371363968651 55 0.8630704453624839 56 0.8727041929469634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 176463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.1972424814267 #Duplication Level Percentage of deduplicated Percentage of total 1 68.63984333273989 26.21852739667806 2 12.05418076078571 9.208729308693608 3 5.870571479437422 6.727189269138573 4 3.4122603999762626 5.213557516306534 5 2.4063853777223905 4.595864288831086 6 1.6497537238146103 3.780962581391 7 1.1453326212094237 3.062398349795708 8 0.8411963681680613 2.570510531952874 9 0.6438787015607382 2.2134951802927527 >10 3.0235594326746185 20.819095221094507 >50 0.20473562399857576 5.429466800405751 >100 0.10088422052103732 7.159007837337006 >500 0.004450774434751647 1.1192147929027614 >1k 0.002967182956501098 1.881980925179783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1847 1.0466783405019748 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1474 0.8353025846778078 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 881 0.4992548012897888 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 571 0.32358058063163386 No Hit TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 523 0.29637941098133885 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 476 0.269744932365425 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 434 0.24594390892141693 No Hit TCGGGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 356 0.20174200823968763 No Hit TCGGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 342 0.1938083337583516 No Hit TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.19210826065520817 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 331 0.18757473238015904 No Hit TCGGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 331 0.18757473238015904 No Hit TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 316 0.17907436686444184 No Hit TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 291 0.16490709100491321 No Hit TCGGGTGGGACAACAGTGTCCTGAGGCCAGCTACTGCCACCAGGAGCAGGAGAGAT 256 0.14507290480157314 No Hit TCGGGTGGGGGGGCTGGTAAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 249 0.1411060675609051 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCC 239 0.13543915721709365 No Hit TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTGTGTG 238 0.1348724661827125 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC 230 0.13033893790766338 No Hit TCGGGTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTC 229 0.1297722468732822 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 229 0.1297722468732822 No Hit TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 228 0.12920555583890106 No Hit TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTCCC 226 0.1280721737701388 No Hit TCGGGTGGGGGGCTGTCAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCTG 221 0.12523871859823307 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 220 0.12467202756385191 No Hit TCGGGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 215 0.12183857239194619 No Hit TCGGGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 212 0.12013849928880274 No Hit TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 210 0.11900511722004047 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCC 208 0.11787173515127816 No Hit TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA 206 0.11673835308251589 No Hit TCGGGTGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT 204 0.1156049710137536 No Hit TCGGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 197 0.11163813377308557 No Hit TCGGGTGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAACACTGACTGGTCTTCCA 197 0.11163813377308557 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCGCTTCC 192 0.10880467860117984 No Hit TCGGGTGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCT 180 0.1020043861886061 No Hit TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGCCTGCTCTTC 178 0.10087100411984382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.001700073103143435 0.0 0.0 0.0 0.0 9 0.001700073103143435 0.0 0.0 0.0 0.0 10 0.004533528275049161 0.0 0.0 0.0 0.0 11 0.009067056550098321 0.0 0.0 0.0 0.0 12 0.009067056550098321 0.0 0.0 0.0 0.0 13 0.011900511722004046 0.0 0.0 0.0 0.0 14 0.01643403999705321 0.0 0.0 0.0 0.0 15 0.018700804134577788 0.0 0.0 0.0 0.0 16 0.020400877237721222 0.0 0.0 0.0 0.0 17 0.021534259306483512 0.0 0.0 0.0 0.0 18 0.021534259306483512 0.0 0.0 0.0 0.0 19 0.021534259306483512 0.0 0.0 0.0 0.0 20 0.025501096547151526 0.0 0.0 0.0 0.0 21 0.02720116965029496 0.0 0.0 0.0 0.0 22 0.0289012427534384 0.0 0.0 0.0 0.0 23 0.034001462062868706 0.0 0.0 0.0 0.0 24 0.03570153516601214 0.0 0.0 0.0 0.0 25 0.036268226200393286 0.0 0.0 0.0 0.0 26 0.039668372406680155 0.0 0.0 0.0 0.0 27 0.040801754475442445 0.0 0.0 0.0 0.0 28 0.04703535585363504 0.0 0.0 0.0 0.0 29 0.04930211999115962 0.0 0.0 0.0 0.0 30 0.0515688841286842 0.0 0.0 0.0 0.0 31 0.05553572136935222 0.0 0.0 0.0 0.0 32 0.056102412403733364 0.0 0.0 0.0 0.0 33 0.05836917654125794 0.0 0.0 0.0 0.0 34 0.05950255861002023 0.0 0.0 0.0 0.0 35 0.0629027048163071 0.0 0.0 0.0 0.0 36 0.06573615998821283 0.0 0.0 0.0 0.0 37 0.06800292412573741 0.0 0.0 0.0 0.0 38 0.06970299722888085 0.0 0.0 0.0 0.0 39 0.07593659860707344 0.0 0.0 0.0 0.0 40 0.07706998067583573 0.0 0.0 0.0 0.0 41 0.0804701268821226 0.0 0.0 0.0 0.0 42 0.0855703461915529 0.0 0.0 0.0 0.0 43 0.09010387446660206 0.0 0.0 0.0 0.0 44 0.09463740274165122 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCGTT 15 0.008030253 50.60167 37 AGTAATC 15 0.008030253 50.60167 37 TAGAGTG 15 0.008057727 50.557983 36 CTAGAGT 15 0.008076081 50.528904 35 TTGGTTG 15 0.008076081 50.528904 34 ACATACT 30 9.697251E-7 50.528904 34 ACGTCCC 25 1.9779909E-5 50.42738 32 AATACTC 15 0.008140565 50.42738 32 TTTCGCA 15 0.008159059 50.398453 31 GCCTAAG 15 0.008159059 50.398453 31 TACTAAC 15 0.008159059 50.398453 31 GCAATTA 15 0.008159059 50.398453 31 GCACTTC 15 0.008289395 50.196857 48 CGTTAGT 15 0.00830814 50.168186 47 TCCGAGT 15 0.008326916 50.139553 40 AGTCTGC 20 4.1171093E-4 50.139553 38 GTCGTAC 20 4.1171093E-4 50.139553 40 CCGCACG 15 0.008326916 50.139553 39 GTACGTA 30 1.0229032E-6 50.139553 39 TACGTAG 30 1.0229032E-6 50.139553 40 >>END_MODULE