FastQCFastQC Report
Fri 17 Jun 2016
SRR1526565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36796
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6791.8453092727470377No Hit
TGAAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.7582345907163822No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.6875747363843896No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.4864659202087183No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.2853571040330471No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC970.263615610392434No Hit
TGAAGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.21741493640613108No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.21741493640613108No Hit
TGAAGCGGGGGCCATTTCGCCGCTGCTTCCATGCGGGTGAGGTGTGTTCTTTCGCCGTTCTCGT740.20110881617567125No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG710.19295575606044135No Hit
TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.17936732253505816No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC620.1684965757147516No Hit
TGAAGCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.16577888900967497No Hit
TGAAGCGGGGAACTTTTCACTGTCTTGTGGTCATCTGGTCTCCTCATTATTTTTATCCCTTCAA570.1549081421893684No Hit
TGAAGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.15219045548429178No Hit
TGAAGCGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.13860202195890858No Hit
TGAAGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.1250135884335254No Hit
TGAAGCGGGGAGACTCTTAATGCCCTAGAGTCAGAGATAACTGAATGAACACCAGTAGACCCAG450.12229590172844874No Hit
TGAAGCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11686052831829546No Hit
TGAAGCGGGTGTTGTAAGGGCACATGGTGGATGCCTTGGCATGCTAAGCCGATGAAGGACGTGG420.11414284161321883No Hit
TGAAGCGGGATATCTATGTGATTAGCCCAGGGAAACTAAGGCTCAGACATGGATAATACCACAG410.1114251549081422No Hit
TGAAGCGGGAAGAGCGTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.10327209479291227No Hit
TGAAGCGGGACAATGGTACAACGCAGAGTCGACTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTT370.10055440808783563No Hit
TGAAGCGGGATCTCCACAGCGCTATGCACACATCTTTCCTAGTTAGAGTAAAATCAGAAAGAAA370.10055440808783563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG201.952989E-458.18878641
GGCAAAC150.004591625658.18878636
TCAAAAG150.004591625658.18878654
AAGGTCC201.952989E-458.18878658
CGTGCGA150.004591625658.18878642
GCCGTGC201.952989E-458.18878640
TTGCCGT150.004591625658.18878638
GAAGGTC150.004591625658.18878657
TGCGAAA150.004616594458.10929544
GCGAAAA150.004616594458.10929545
GTGCGAA150.004616594458.10929543
TATGCGT150.00466683557.9509632
ATGCGTT150.00466683557.9509633
CGGGGAC550.057.8721126
AAGAGTG258.616484E-657.87211210
GAATGGA258.616484E-657.8721129
CGGGTAC258.616484E-657.8721126
GCAGTGA258.616484E-657.87211212
GGAAGCT258.616484E-657.8721128
GGGGACA258.616484E-657.8721127
Produced by FastQC (version 0.11.5)